Cloning, expression and characterization of glycoside hydrolase family 11 endoxylanase from Bacillus pumilus ARA

Biotechnology Letters

Volumn 33, Issue 7, 2011, Pages 1407-1416

  Qu, Wei ^a   Shao, Weilan ^a  

^a NANJING NORMAL UNIVERSITY   (China)

Author keywords

Bacillus pumilus; Characterization; Gene cloning and expression; Thermo tolerant bacterium; Xylan degradation; Xylanase

Indexed keywords

BACILLUS PUMILUS; GENE CLONING AND EXPRESSION; THERMO-TOLERANT BACTERIUM; XYLAN DEGRADATION; XYLANASES;

BACILLI; BACTERIOLOGY; CHROMATOGRAPHIC ANALYSIS; CLONING; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GENE ENCODING; GENE EXPRESSION; HYDROLASES; ION CHROMATOGRAPHY; ION EXCHANGE; METAL IONS; PURIFICATION; SUGARS;

ENZYME ACTIVITY;

BACTERIAL DNA; ENDO 1,4 BETA XYLANASE; ENZYME ACTIVATOR; ENZYME INHIBITOR; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; XYLAN;

5' UNTRANSLATED REGION; AMINO ACID SEQUENCE; ARTICLE; BACILLUS; CHEMISTRY; CONFORMATION; DNA SEQUENCE; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; SEQUENCE HOMOLOGY; TEMPERATURE; TIME;

5' UNTRANSLATED REGIONS; AMINO ACID SEQUENCE; BACILLUS; CLONING, MOLECULAR; DNA, BACTERIAL; ENDO-1,4-BETA XYLANASES; ENZYME ACTIVATORS; ENZYME INHIBITORS; ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; NUCLEIC ACID CONFORMATION; OPEN READING FRAMES; PROTEIN SORTING SIGNALS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; TEMPERATURE; TIME FACTORS; XYLANS;

BACILLUS PUMILUS; BACTERIA (MICROORGANISMS); BIFIDOBACTERIUM; ESCHERICHIA COLI; TRITICUM AESTIVUM SUBSP. SPELTA;

EID: 79958772404     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0568-x     Document Type: Article

References (23)

1. Beg QK, Kapoor M, Mahajan L, Hoondal GS (2001) Microbial xylanases and their industrial applications. Appl Microbiol Biotechnol 56: 326-338.
2. Biely P (1985) Microbial xylanolytic systems. Trends Biotechnol 3: 286-290.
3. Biely P, Vrsanská M, Tenkanen M, Kluepfel D (1997) Endo-β-1, 4-xylanase families: differences in catalytic properties. J Biotechnol 57: 151-166.
4. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
5. Coughlan MP, Hazlewood GP (1993) β-1, 4-d-xylan degrading enzyme systems: biochemistry, molecular biology and applications. Biotechnol Appl Biochem 17: 259-289.
6. Crittenden RG, Playne MJ (1996) Production, properties and applications of food-grade oligosaccharides. Trends Food Sci Technol 7: 353-361.
7. Huang J, Wang G, Xiao L (2006) Cloning, sequencing and expression of the xylanase gene from a Bacillus subtilis strain B10 in Escherichia coli. Bioresour Technol 97: 802-808.
8. Jalal A, Rashid N, Rasool N, Akhtar M (2009) Gene cloning and characterization of a xylanase from a newly isolated Bacillus subtilis strain R5. J Biosci Bioeng 107(4): 360-365.
9. Khandeparker R, Numan MT (2008) Bifunctional xylanases and their potential use in biotechnology. J Ind Microbiol Biotechnol 35: 635-644.
10. Khasin A, Alchanati I, Shoham Y (1993) Purification and characterization of a thermostable xylanase from Bacillus stearothermophilus T-6. Appl Environ Microbiol 59(6): 1725-1730.
11. Kuhad RC, Singh A (1993) Lignocellulose biotechnology: current and future prospects. Crit Rev Biotech 13: 151-172.
12. Lever M (1972) A new reaction for colorimetric determination of carbohydrates. Anal Biochem 47: 273-279.
13. López C, Blanco A, Pastor FIJ (1998) Xylanase production by a new alkali-tolerant isolate of Bacillus. Biotechnol Lett 20: 243-246.
14. Morales P, Madarro A, Flors A, Sendra J, Gonzalez JP (1995) Purification and characterization of a xylanase and an arabinofuranosidase from Bacillus polymyxa. Enzyme Microb Technol 17: 424-429.
15. Pei J, Shao W (2008) Purification and characterization of an extracellular α-l-arabinosidase from a novel isolate Bacillus pumilus ARA and its over-expression in Escherichia coli. Appl Microbiol Biotechnol 78: 115-121.
16. Petrescu I, Brasseur JL, Chessa JP, Ntarima P, Claeyssens M, Devreese B, Marino G, Gerday C (2000) Xylanase from the psychrophilic yeast Cryptococcus adeliae. Extremophiles 4: 137-144.
17. Singh S, Madlala AM, Prior BA (2003) Thermomyces lanuginosus: properties of strains and their hemicellulases. FEMS Microbiol Rev 27: 3-16.
18. Wu H, Pei J, Jiang Y, Song X, Shao W (2010) pHsh vectors, a novel expression system of Escherichia coli for the large-scale production of recombinant enzymes. Biotechnol Lett 32: 795-801.
19. Xue Y, Wu A, Zeng H, Shao W (2006) High-level expression of an α-arabinosidase from Thermotoga maritime in Escherichia coli for the production of xilobiose from xylan. Biotechnol Lett 28: 351-356.
20. Yamaura I, Koga T, Matsumoto T, Kato T (1997) Purification and some properties of endo-1, 4-β-d-xylanase from a fresh-water mollusc, Pomacea insularus (de Ordigny). Biosci Biotechnol Biochem 61: 615-620.
21. Yang SQ, Yan QJ, Jiang ZQ, Li LT, Tian HM, Wang YZ (2006) High-level of xylanase production by the thermophilic Paecilomyces thermophila J18 on wheat straw in solid-state fermentation. Biore Technol 97: 1794-1800.
22. Yin E, Le Y, Pei J, Shao W, Yang Q (2008) High-level expression of the xylanase from Thermomyces lanuginosus in Escherichia coli. World J Microbiol Biotechnol 24: 275-280.
23. Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31: 3406-3415.