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Volumn 21, Issue 13, 2011, Pages 3962-3965

Selective accumulation of hybrid liposomes into adult T-cell leukemia cells along with induction of apoptosis

Author keywords

Adult T cell leukemia cell; Apoptosis; Hybrid liposomes; Membrane fluidity

Indexed keywords

CASPASE 3; DIMYRISTOYLPHOSPHATIDYLCHOLINE; HYBRID LIPOSOME; LIPOSOME; POLIDOCANOL; UNCLASSIFIED DRUG;

EID: 79958755326     PISSN: 0960894X     EISSN: 14643405     Source Type: Journal    
DOI: 10.1016/j.bmcl.2011.05.015     Document Type: Article
Times cited : (6)

References (25)
  • 24
    • 79958712138 scopus 로고    scopus 로고
    • note
    • 2, 10 mM KCl, 0.1% NP-40, 0.5 mM DTT, 0.5 mM PMSF, 2 μg/ml pepstatin A, 2 μg/ml aprotinin and 2 μg/ml leupeptin). After the incubation on ice for 10 min, the samples were vortexed for 10 s and centrifuged at 5000 rpm for 1 min. Then the supernatant was collected as a cytoplasmic sample. Then the cytoplasmic protein (40 μg protein) was separated by 12% SDS-PAGE and blotted onto a PVDF membrane (GE Healthcare, Tokyo, Japan). The detection was performed using ECL Plus Western Blotting Detection System (ECL, GE Healthcare Bio-Science, Buckinghamshire, UK). Primary antibodies used were as follows: anti-Caspase3 (#9661S) (Cell Signaling Technology, Danvers, MA, USA) and anti-Actin (C-2)(Santa Cruz Biotechnology, Santa Cruz, CA, USA). Second antibody used was ECL anti-rabbit IgG Horse Radish Peroxidase linked whole antibody (GE Healthcare Bio-Science, Buckinghamshire, UK).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.