Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag

Methods in Molecular Biology

Volumn 712, Issue , 2011, Pages 21-30

  Qi, Yiping ^a   Katagiri, Fumiaki ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Arabidopsis; Biotinylation; HPB tag; Plasma membrane; Protein complexes; R protein

Indexed keywords

ARABIDOPSIS PROTEIN; NUCLEAR PROTEIN;

AFFINITY LABELING; ARABIDOPSIS; ARTICLE; BIOTINYLATION; CHEMISTRY; GENETICS; INNATE IMMUNITY; ISOLATION AND PURIFICATION; PHYSIOLOGY; PLANT IMMUNITY; TRANSGENIC PLANT;

AFFINITY LABELS; ARABIDOPSIS; ARABIDOPSIS PROTEINS; BIOTINYLATION; IMMUNITY, INNATE; NUCLEAR PROTEINS; PLANT IMMUNITY; PLANTS, GENETICALLY MODIFIED;

EID: 79958289724     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61737-998-7_3     Document Type: Chapter

References (16)

1. Dangl, J. L., and Jones, J. D. (2001) Plant pathogens and integrated defence responses to infection. Nature. 411, 826–833.
2. Jones, J. D., and Dangl, J. L. (2006) The plant immune system. Nature. 444, 323–329.
3. Jia, Y., McAdams, S. A., Bryan, G. T., Hershey, H. P., and Valent, B. (2000) Direct interaction of resistance gene and avirulence gene products confers rice blast resistance. EMBO J. 19, 4004–4014.
4. Deslandes, L., Olivier, J., Peeters, N., Feng, D. X., Khounlotham, M., Boucher, C., Somssich, I., Genin, S., and Marco, Y. (2003) Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus. Proc Natl Acad Sci USA. 100, 8024–8029.
5. Shao, F., Golstein, C., Ade, J., Stoutemyer, M., Dixon, J. E., and Innes, R. W. (2003) Cleavage of Arabidopsis PBS1 by a bacterial type III effector. Science. 301, 1230–1233.
6. Axtell, M. J., and Staskawicz, B. J. (2003) Initiation of RPS2-specified disease resistance in Arabidopsis is coupled to the AvrRpt2-directed elimination of RIN4. Cell. 112, 369–377.
7. Mackey, D., Holt, B. F., Wiig, A., and Dangl, J. L. (2002) RIN4 interacts with Pseudomonas syringae type III effector molecules and is required for RPM1-mediated resistance in Arabidopsis. Cell. 108, 743–754.
8. Mackey, D., Belkhadir, Y., Alonso, J. M., Ecker, J. R., and Dangl, J. L. (2003) Arabidopsis RIN4 is a target of the type III virulence effector AvrRpt2 and modulates RPS2-mediated resistance. Cell. 112, 379–389.
9. Leister, R. T., and Katagiri, F. (2000) A resistance gene product of the nucleotide binding site – leucine rich repeats class can form a complex with bacterial avirulence proteins in vivo. Plant J. 22, 345–354.
10. Belkhadir, Y., Subramaniam, R., and Dangl, J. L. (2004) Plant disease resistance protein signaling: NBS-LRR proteins and their partners. Curr Opin Plant Biol. 7, 391–399.
11. Boyes, D. C., Nam, J., and Dangl, J. L. (1998) The Arabidopsis thaliana RPM1 disease resistance gene product is a peripheral plasma membrane protein that is degraded coincident with the hypersensitive response. Proc Natl Acad Sci USA. 95, 15849–15854.
12. Holt, B. F., III, Belkhadir, Y., and Dangl, J. L. (2005) Antagonistic control of disease resistance protein stability in the plant immune system. Science. 309, 929–932.
13. Tian, D., Traw, M. B., Chen, J. Q., Kreitman, M., and Bergelson, J. (2003) Fitness costs of R-gene-mediated resistance in Arabidopsis thaliana. Nature. 423, 74–77.
14. Qi, Y., and Katagiri, F. (2009) Purification of low-abundance Arabidopsis plasma-mem-brane protein complexes and identification of candidate components. Plant J. 57, 932–944.
15. Mersereau, M., Pazour, G. J., and Das, A. (1990) Efficient transformation of Agrobacterium tumefaciens by electroporation. Gene. 90, 149–151.
16. Clough, S. J., and Bent, A. F. (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 16, 735–743.