Fragment Complementation and Co-immunoprecipitation Assays for Understanding R Protein Structure and Function

Methods in Molecular Biology

Volumn 712, Issue , 2011, Pages 9-20

  Moffett, Peter ^a  

^a United States Department of Agriculture Agricultural Research Service and Boyce Thompson Institute for Plant Research   (United States)

Author keywords

Agrobacterium; Agroinfiltration; Immunoprecipitation; NB LRR; Nicotiana benthamiana; Transient expression

Indexed keywords

NUCLEAR PROTEIN; VEGETABLE PROTEIN;

ARTICLE; CHEMISTRY; GENETIC COMPLEMENTATION; GENETIC TRANSFORMATION; GENETICS; IMMUNOPRECIPITATION; INNATE IMMUNITY; METABOLISM; METHODOLOGY; MICROBIOLOGY; PHYSIOLOGY; PLANT IMMUNITY; RHIZOBIUM RADIOBACTER; TOBACCO;

GENETIC COMPLEMENTATION TEST; IMMUNITY, INNATE; IMMUNOPRECIPITATION; NUCLEAR PROTEINS; PLANT IMMUNITY; PLANT PROTEINS; RHIZOBIUM RADIOBACTER; TOBACCO; TRANSFORMATION, GENETIC;

EID: 79958250847     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61737-998-7_2     Document Type: Chapter

References (17)

1. Hellens, R., Mullineaux, P., and Klee, H. (2000) Technical Focus: a guide to Agrobacterium binary Ti vectors, Trends Plant Sci 5, 446–451.
2. Wroblewski, T., Tomczak, A., and Michelmore, R. (2005) Optimization of Agrobacterium-mediated transient assays of gene expression in lettuce, tomato and Arabidopsis, Plant Biotechnol J 3, 259–273.
3. Voinnet, O., Rivas, S., Mestre, P., and Baulcombe, D. (2003) An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus, Plant J 33, 949–956.
4. McDowell, J. M., and Simon, S. A. (2008) Molecular diversity at the plant–pathogen interface, Dev Comp Immunol 32, 736–744.
5. Scofield, S. R., Tobias, C. M., Rathjen, J. P., Chang, J. H., Lavelle, D. T., Michelmore, R. W., and Staskawicz, B. J. (1996) Molecular basis of gene-for-gene specificity in bacterial speck disease of tomato, Science 274, 2063–2065.
6. Tang, X., Frederick, R. D., Zhou, J., Halterman, D. A., Jia, Y., and Martin, G. B. (1996) Initiation of plant disease resistance by physical interaction of AvrPto and Pto kinase, Science 274, 2060–2063.
7. Bendahmane, A., Querci, M., Kanyuka, K., and Baulcombe, D. C. (2000) Agrobacterium transient expression system as a tool for the isolation of disease resistance genes: application to the Rx2 locus in potato, Plant J 21, 73–81.
8. Moffett, P., Farnham, G., Peart, J., and Baulcombe, D. C. (2002) Interaction between domains of a plant NBS-LRR protein in disease resistance-related cell death, EMBO J 21, 4511–4519.
9. Leister, R. T., Dahlbeck, D., Day, B., Li, Y., Chesnokova, O., and Staskawicz, B. J. (2005) Molecular genetic evidence for the role of SGT1 in the intramolecular complementation of Bs2 protein activity in Nicotiana benthamiana, Plant Cell 17,
10. –1278.
11. Rairdan, G. J., Collier, S. M., Sacco, M. A., Baldwin, T. T., Boettrich, T., and Moffett, P. (2008) The coiled-coil and nucleotide binding domains of the potato Rx disease resistance protein function in pathogen recognition and signaling, Plant Cell 20, 739–751.
12. Rairdan, G. J., and Moffett, P. (2006) Distinct domains in the ARC region of the potato resistance protein Rx mediate LRR binding and inhibition of activation, Plant Cell 18, 2082–2093.
13. Sacco, M. A., Mansoor, S., and Moffett, P. (2007) A RanGAP protein physically interacts with the NB-LRR protein Rx, and is required for Rx-mediated viral resistance, Plant J 52, 82–93.
14. Tameling, W. I., and Baulcombe, D. C. (2007) Physical association of the NB-LRR resistance protein Rx with a Ran GTPase-activating protein is required for extreme resistance to potato virus X, Plant Cell 19, 1682–1694.
15. Komori, T., Imayama, T., Kato, N., Ishida, Y., Ueki, J., and Komari, T. (2007) Current status of binary vectors and superbinary vectors, Plant Physiol 145, 1155–1160.
16. Hamilton, C. M., Frary, A., Lewis, C., and Tanksley, S. D. (1996) Stable transfer of intact high molecular weight DNA into plant chromosomes, Proc Natl Acad Sci U S A 93, 9975–9979.
17. Scopes, R. K., and Smith, J. A. (2006) Analysis of Proteins, in Current Protocols in Molecular Biology (Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K., Eds.), pp 10.10.11–10.18.28, Wiley, New York.