Effects of coccidiosis control programs on antibody levels against selected pathogens and serum nitric oxide levels in broiler chickens

Journal of Applied Poultry Research

Volumn 20, Issue 2, 2011, Pages 143-152

  Lee, K W ^a   Lillehoj, H S ^a   Jang, S I ^a   Li, G X ^a   Bautista, D A ^b   Phillips, K ^b   Ritter, D ^c   Lillehoj, E P ^d   Siragusa, G R ^e  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b UNIVERSITY OF DELAWARE   (United States)

^c MOUNTAIRE FARMS INC   (United States)

^d UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^e Danisco   (United States)

Author keywords

Chicken anemia virus; Clostridium; Coccidiosis control program; Infectious bursal disease virus; Reovirus

Indexed keywords

AVES; BACTERIA (MICROORGANISMS); CHICKEN ANEMIA VIRUS; CLOSTRIDIUM; COCCIDIA; GALLUS GALLUS; GYROVIRUS; INFECTIOUS BURSAL DISEASE VIRUS; ORTHOREOVIRUS;

EID: 79958214001     PISSN: 10566171     EISSN: None     Source Type: Journal    
DOI: 10.3382/japr.2010-00218     Document Type: Article

References (45)

1. USDA-Foreign Agricultural Service. 2009. Livestock and poultry: World markets and trade. Accessed March 2010. http://www.fas.usda.gov/dlp/circular/2009/livestock_poultry_10-2009.pdf.
2. Dekich, M. A. 1998. Broiler industry strategies for control of respiratory and enteric diseases. Poult. Sci. 77:1176-1180.
3. Smith, J. A., and J. D. Helm. 2008. Report of the committee on transmissible diseases of poultry and other avian species. Accessed May 2009. http://www.usaha.org/committees/reports/2008/report-pad-2008.pdf.
4. Williams, R. B. 1999. A compartmentalised model for the estimation of the cost of coccidiosis to the world's chicken production industry. Int. J. Parasitol. 29:1209-1229.
5. Sun, X. M., W. Pang, T. Jia, W. C. Yan, G. He, L. L. Hao, M. Bentue, and X. Sue. 2009. Prevalence of Eimeria species in broilers with subclinical signs from fifty farms. Avian Dis. 53:301-305.
6. Nematollahi, A., G. Moghaddam, and R. F. Pourabad. 2009. Prevalence of Eimeria species among broiler chickens in Tabriz (northwest of Iran). Mun. Ent. Zoo. 4:53-58.
7. Williams, R. B., A. C. Bushell, J. M. Reperant, T. G. Doy, J. H. Morgan, M. W. Shirley, P. Yvore, M. M. Carr, and Y. Fremont. 1996. A survey of Eimeria species in commercially- reared chickens in France during 1994. Avian Pathol. 25:113-130.
8. Al-Natour, M. Q., M. M. Suleiman, and M. N. Abo-Shehada. 2002. Flock-level prevalence of Eimeria species among broiler chicks in northern Jordan. Prev. Vet. Med. 53:305-310.
9. Haug, A., A. Gjevre, P. Thebo, J. G. Mattson, and M. Kaldhusdal. 2008. Coccidial infections in commercial broilers: Epidemiological aspects and comparison of Eimeria species identification by morphometric and polymerase chain reaction techniques. Avian Pathol. 37:161-170.
10. Lee, B. H., W. H. Kim, J. Jeong, J. Yoo, Y. K. Kwon, B. Y. Jung, J. H. Kwon, H. S. Lillehoj, and W. Min. 2010. Prevalence and cross-immunity of Eimeria species on Korean chicken farms. J. Vet. Med. Sci. 72:985-989.
11. Williams, R. B. 2005. Intercurrent coccidiosis and necrotic enteritis of chickens: Rational, integrated disease management by maintenance of gut integrity. Avian Pathol. 34:159-180.
12. Lee, J. T., C. Broussard, S. Fitz-Coy, P. Burke, N. H. Eckert, S. M. Stevens, P. N. Anderson, S. M. Anderson, and D. J. Caldwell. 2009. Evaluation of live oocyst vaccination or salinomycin for control of field-strain Eimeria challenge in broilers on two different feeding programs. J. Appl. Poult. Res. 18:458-464.
13. Munir, K., M. A. Muneer, A. Tiwari, R. M. Chaudhry, and S. Muruganandan. 2007. Effects of polyether ionophores on the protective immune responses of broiler chickens against Angara disease and Newcastle disease virus. Vet. Res. Commun. 31:909-929.
14. Ritter, G. 2006. Proposed pathogenesis of gangrenous dermatitis in chickens and attempts at experimental reproduction. Pages 59-66 in Proc. 41st Natl. Mtg. Poult. Health Lee et al.: IN-FEED ANTIMICROBIALS IN BROILERS 151 Processing. Ocean City, MD. Delmarva Poultry Industry, Georgetown, DE.
15. Li, G. X., H. S. Lillehoj, K. W. Lee, S. I. Jang, P. Marc, C. G. Gay, D. Ritter, D. A. Bautista, K. Phillips, A. P. Neumann, T. G. Rehberger, and G. R. Siragusa. 2010. An outbreak of gangrenous dermatitis in commercial broiler chickens. Avian Pathol. 39:247-253.
16. Vaccines reported in this study were as follows: turkey herpes virus and SB-1 (Merial), Rispen (CEVA, Lenexa, KS), Newcastle disease and infectious bronchitis (Merial), infectious bursal disease virus and reovirus (Merial, Gainesville, GA) at 12 wk and (CEVA) at 20 wk, and chicken anemia virus (CEVA).
17. The AGP and coccidiostats used in this study were nicarbazine (Nicarb, Phibro, Ridgefield Park, NJ), salinomycin (Bio-Cox, Alpharma Inc., Fort Lee, NJ), diclazuril (Clinacox, Schering-Plough, Kenilworth, NJ), decoquinate (Deccox, Alpharma Inc.), monensin (Coban, Elanco, Greenfield, IN), nicarbazin plus narasin (Maxiban, Elanco), bacitracin methylene disalicylate (BMD, Alpharma Inc.), roxarsone (3-nitro, Alpharma Inc.), virginiamycin (Stafac, Phibro Animal Health, Fairfield, NJ).
18. Inovocox vaccine, Pfizer, Durham, NC. In ovo vaccination was performed using Inovocox, Marek's disease vaccine, or both as recommended by the manufacturer (Embrex Inovoject System, Pfizer, Durham, NC).
19. Serum (100 μL) was mixed with an equal volume of freshly prepared Griess reagent (Sigma, St. Louis, MO) containing 1% (wt/vol) sulfanilamine in 5% phosphoric acid and 0.1% N-naphthylethylenediamine, the mixture was incubated for 10 min at room temperature, and the optical density at 540 nm was determined with an automated microtiter plate reader. Nitrite concentrations were calculated from a standard curve generated with NaNO2.
20. Flat-bottom 96-well microtiter plates (Corning Costar, Corning, NY) were coated overnight at 4°C with 100 μL/well of whole bacterial lysates of C. perfringens or C. septicum (10 μg/mL) in 0.1 M carbonate buffer, pH 9.6. Crude soluble clostridial antigens were prepared from C. perfringens and C. septicum cultures using a previously published protocol [Kulkarni, R. R., V. R. Parreira, S. Sharif, and J. F. Prescott. 2008. Oral immunization of broiler chickens against necrotic enteritis with an attenuated Salmonella vaccine vector expressing Clostridium perfringens antigens. Vaccine 26:4194-4203]. Plates were washed 3 times with PBS containing 0.05% Tween 20 (PBS-T), blocked with 200 μL/well of PBS containing 1% BSA for 1 h at room temperature, washed with PBS-T, and incubated with 100μL/well of appropriately diluted serum samples for 2 h at room temperature. After washing with PBS-T, horseradish peroxidase-conjugated goat anti-chicken IgG antibody (1:5,000; Sigma, St. Louis, MO) was added, and bound antibodies were detected at 450 nm after reaction with 0.01% tetramethylbenzidine (Sigma) in 0.05 M phosphate-citrate buffer, pH 5.0, using a microplate reader (Bio-Rad, Richmond, CA).
21. IDEXX Laboratories, Westbrook, MA.
22. SPSS Inc., Chicago, IL.
23. Sims, M. D., K. A. Dawson, K. E. Newman, P. Spring, and D. M. Hooge. 2004. Effects of dietary mannan oligosaccharide, bacitracin methylene disalicylate, or both on the live performance and intestinal microbiology of turkey. Poult. Sci. 83:1148-1154.
24. Watkins, K. L., T. R. Shryock, R. N. Dearth, and Y. M. Saif. 1997. In vitro antimicrobial susceptibility of Clostridium perfringens from commercial turkey and broiler chicken origin. Vet. Microbiol. 54:195-200.
25. Martel, A., L. A. Devriese, K. Cauwerts, K. De Gussem, A. Decostere, and F. Haesebrouck. 2004. Susceptibilityof Clostridium perfringens strains from broiler chickens to antibiotics and anticoccidials. Avian Pathol. 33:3-7.
26. Johansen, C. H., L. Bjerrum, and K. Pedersen. 2007. Impact of salinomycin on the intestinal microflora of broiler chickens. Acta Vet. Scand. 49:30. doi:10.1186/1751-0147- 49-30.
27. Benno, Y., K. Endo, and T. Mutsuoka. 1988. Isolation of fecal Clostridium perfringens from broiler chickens and their susceptibility to eight antimicrobial agents for growth promotion. Jpn. J. Vet. Sci. 50:832-834.
28. Mulligan, L. T. 2009. Antiprotozoan agent. Diclazuril. Accessed May 2010. http://www.inchem.org/documents/jecfa/jecmono/v36je07.htm.
29. Hansen, M., K. A. Krogh, A. Brandt, J. H. Christensen, and B. Halling-Sorensen. 2009. Fate and antibacterial potency of anticoccidial drugs and their main abiotic degradation products. Environ. Pollut. 157:474-480.
30. Johnson, S. 1997. Antibody responses to clostridial infection in humans. Clin. Infect. Dis. 25(Suppl. 2):S173- S177.
31. Lovland, A., M. Kaldhusdal, and L. J. Reitan. 2003. Diagnosing Clostridium perfringens-associated necrotic enteritis in broiler flocks by an immunoglobulin G anti-alphatoxin enzyme-linked immunosorbent assay. Avian Pathol. 32:527-534.
32. Lee, K. W., H. S. Lillehoj, G. X. Li, M.-S. Park, S. I. Jang, W. S. Jeong, H.-Y. Jeoung, D.-J. An, and E. P. Lillehoj. 2011. Identification and cloning of two immunogenic Clostridium perfringens proteins, elongation factor Tu (EF-Tu) and pyruvate:ferredoxin oxidoreductase (PFO) of C. perfringens. Res. Vet. Sci. doi:10.1016/j.rvsc.2011.01.017.
33. Heier, B. T., A. Lovland, K. B. Soleim, M. Kaldhusdal, and J. Jarp. 2001. A field study of naturally occurring specific antibodies against Clostridium perfringens alphatoxin in Norwegian broiler flocks. Avian Dis. 45:724-732.
34. Knarreborg, A., M. A. Simon, R. M. Engberg, B. B. Jensen, and G. W. Tannock. 2002. Effects of dietary fat source and subtherapeutic levels of antibiotic on the bacterial community in the ileum of broiler chickens at various ages. Appl. Environ. Microbiol. 68:5918-5924.
35. Wise, M. G., and G. R. Siragusa. 2007. Quantitative analysis of the intestinal bacterial community in one- to three-week-old commercially reared broiler chickens fed conventional or antibiotic-free vegetable-based diets. J. Appl. Microbiol. 102:1138-1149.
36. Kim, J. M., M. J. Kim, J. S. Song, and I. P. Mo. 2008. Epidemiological studies of avian reovirus infection in broilers in Korea. Kor. J. Poult. Sci. 35:85-99.
37. Toro, H., L. van Santen, F. J. Hoerr, and C. Breedlove. 2009. Effects of chicken anemia virus and infectious bursal disease virus in commercial chickens. Avian Dis. 53:94-102.
38. Lillehoj, H. S., and G. Li. 2004. Nitric oxide production by macrophages stimulated with coccidia sporozoites, lipopolysaccharide, or interferon-γ, and its dynamic changes in SC and TK strains of chickens infected with Eimeria tenella. Avian Dis. 48:244-253.
39. Allen, P. C. 1997. Nitric oxide production during Eimeria tenella infections in chickens. Poult. Sci. 76:810- 813. 152 JAPR: Research Report
40. Babu, U. S., D. W. Gaines, H. Lillehoj, and R. B. Raybourne. 2006. Differential reactive oxygen and nitrogen production and clearance of Salmonella serovars by chicken and mouse macrophage. Dev. Comp. Immunol. 30:942- 953.
41. Thaxton, J. P., S. A. Cutler, R. Griffith, and C. G. Scanes. 2006. Changes in tissue nitrite concentration in the crop of the turkey poult in response to Salmonella Typhimurium challenge. Poult. Sci. 85:1015-1019.
42. Korhonen, R., R. Korpela, M. Saxelin, M. Mäki, H. Kankaanranta, and E. Moilanen. 2001. Induction of nitric oxide synthesis by probiotic Lactobacillus rhamnosus GG in J774 macrophages and human T84 intestinal epithelial cells. Inflammation 25:223-232.
43. Hemmrich, K., K. D. Kröncke, C. V. Suschek, and V. Kolb-Bachofen. 2005. What sense lies in antisense inhibition of inducible nitric oxide synthase expression? Nitric Oxide 12:183-199.
44. Enocksson, A., J. Lundberg, E. Weitzberg, A. Norrby- Teglund, and B. Svenungsson. 2004. Rectal nitric oxide gas and stool cytokine levels during the course of infectious gastroenteritis. Clin. Diagn. Lab. Immunol. 11:250-254.
45. Engberg, R. M., M. S. Hedemann, T. D. Leser, and B. B. Jensen. 2000. Effect of zinc bacitracin and salinomycin on intestinal microflora and performance of broilers. Poult. Sci. 79:1311-1319.