Phenylbutyrate counteracts Shigella mediated downregulation of cathelicidin in rabbit lung and intestinal epithelia: A potential therapeutic strategy

PLoS ONE

Volumn 6, Issue 6, 2011, Pages

  Sarker, Protim ^a,b   Ahmed, Sultan ^a   Tiash, Snigdha ^a   Rekha, Rokeya Sultana ^a,b   Stromberg, Roger ^c   Andersson, Jan ^c   Bergman, Peter ^c   Gudmundsson, Gudmundur H ^d   Agerberth, Birgitta ^b   Raqib, Rubhana ^a  

^a INTERNATIONAL CENTRE FOR DIARRHOEAL DISEASE RESEARCH   (Bangladesh)

^b KAROLINSKA INSTITUTE   (Sweden)

^c KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^d UNIVERSITY OF ICELAND   (Iceland)

Author keywords

[No Author keywords available]

Indexed keywords

4 PHENYLBUTYRIC ACID; ALANINE AMINOTRANSFERASE; ASPARTATE AMINOTRANSFERASE; BUTYRIC ACID; CATHELICIDIN; CATHELICIDIN ANTIMICROBIAL PEPTIDE 18; CREATININE; MESSENGER RNA; POLYPEPTIDE ANTIBIOTIC AGENT; UNCLASSIFIED DRUG; UREA; ANTIMICROBIAL CATIONIC PEPTIDE; ARYLBUTYRIC ACID DERIVATIVE; CAP18 LIPOPOLYSACCHARIDE BINDING PROTEIN; CAP18 LIPOPOLYSACCHARIDE-BINDING PROTEIN;

ALANINE AMINOTRANSFERASE BLOOD LEVEL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ASPARTATE AMINOTRANSFERASE BLOOD LEVEL; BACTERIAL LOAD; COLON MUCOSA; CONTROLLED STUDY; CREATININE BLOOD LEVEL; DISEASE SEVERITY; DOWN REGULATION; DRUG EFFICACY; DRUG SAFETY; FECES LEVEL; FEMALE; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; INNATE IMMUNITY; INTESTINE EPITHELIUM; LUNG ALVEOLUS EPITHELIUM; MALE; NONHUMAN; OUTCOME ASSESSMENT; PROTEIN EXPRESSION; PROTEIN SECRETION; RABBIT; REAL TIME POLYMERASE CHAIN REACTION; RECTUM MUCOSA; RESPIRATORY TRACT INFECTION; SHIGELLA FLEXNERI; SHIGELLOSIS; TRACHEA MUCOSA; UREA BLOOD LEVEL; WESTERN BLOTTING; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; DRUG EFFECT; FECES; GENETICS; INTESTINE MUCOSA; LARGE INTESTINE; LUNG; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; ORAL DRUG ADMINISTRATION; PATHOGENICITY; SECRETION; SHIGELLA; TRACHEA;

ORYCTOLAGUS CUNICULUS; SHIGELLA;

ADMINISTRATION, ORAL; AMINO ACID SEQUENCE; ANIMALS; ANTIMICROBIAL CATIONIC PEPTIDES; BACTERIAL LOAD; DOWN-REGULATION; DYSENTERY, BACILLARY; FECES; INTESTINAL MUCOSA; INTESTINE, LARGE; LUNG; MOLECULAR SEQUENCE DATA; PHENYLBUTYRATES; RABBITS; RNA, MESSENGER; SHIGELLA; TRACHEA;

EID: 79958041855     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0020637     Document Type: Article

References (35)

1. Zasloff M, (2002) Antimicrobial peptides of multicellular organisms. Nature 415: 389-395.
2. Islam D, Bandholtz L, Nilsson J, Wigzell H, Christensson B, et al. (2001) Downregulation of bactericidal peptides in enteric infections: a novel immune escape mechanism with bacterial DNA as a potential regulator. Nat Med 7: 180-185.
3. Sperandio B, Regnault B, Guo J, Zhang Z, Stanley SL Jr, et al. (2008) Virulent Shigella flexneri subverts the host innate immune response through manipulation of antimicrobial peptide gene expression. J Exp Med 205: 1121-1132.
4. Chakraborty K, Ghosh S, Koley H, Mukhopadhyay AK, Ramamurthy T, et al. (2008) Bacterial exotoxins downregulate cathelicidin (hCAP-18/LL-37) and human beta-defensin 1 (HBD-1) expression in the intestinal epithelial cells. Cell Microbiol 10: 2520-2537.
5. Bergman P, Johansson L, Asp V, Plant L, Gudmundsson GH, et al. (2005) Neisseria gonorrhoeae downregulates expression of the human antimicrobial peptide LL-37. Cell Microbiol 7: 1009-1017.
6. Otte JM, Zdebik AE, Brand S, Chromik AM, Strauss S, et al. (2009) Effects of the cathelicidin LL-37 on intestinal epithelial barrier integrity. Regul Pept 156: 104-117.
7. Shaykhiev R, Beisswenger C, Kandler K, Senske J, Puchner A, et al. (2005) Human endogenous antibiotic LL-37 stimulates airway epithelial cell proliferation and wound closure. Am J Physiol Lung Cell Mol Physiol 289: L842-848.
8. Palffy R, Gardlik R, Behuliak M, Kadasi L, Turna J, et al. (2009) On the physiology and pathophysiology of antimicrobial peptides. Mol Med 15: 51-59.
9. Hase K, Eckmann L, Leopard JD, Varki N, Kagnoff MF, (2002) Cell differentiation is a key determinant of cathelicidin LL-37/human cationic antimicrobial protein 18 expression by human colon epithelium. Infect Immun 70: 953-963.
10. Schauber J, Svanholm C, Termen S, Iffland K, Menzel T, et al. (2003) Expression of the cathelicidin LL-37 is modulated by short chain fatty acids in colonocytes: relevance of signalling pathways. Gut 52: 735-741.
11. Raqib R, Sarker P, Bergman P, Ara G, Lindh M, et al. (2006) Improved outcome in shigellosis associated with butyrate induction of an endogenous peptide antibiotic. Proc Natl Acad Sci U S A 103: 9178-9183.
12. Steinmann J, Halldorsson S, Agerberth B, Gudmundsson GH, (2009) Phenylbutyrate induces antimicrobial peptide expression. Antimicrob Agents Chemother 53: 5127-5133.
13. Bennish ML, (1991) Potentially lethal complications of shigellosis. Rev Infect Dis 13 (Suppl 4): S319-324.
14. Butler T, Dunn D, Dahms B, Islam M, (1989) Causes of death and the histopathologic findings in fatal shigellosis. Pediatr Infect Dis J 8: 767-772.
15. Dutta P, Mitra U, Rasaily R, Bhattacharya SK, Bhattacharya MK, et al. (1995) Assessing the cause of in-patients pediatric diarrheal deaths: an analysis of hospital records. Indian Pediatr 32: 313-321.
16. Egorin MJ, Yuan ZM, Sentz DL, Plaisance K, Eiseman JL, (1999) Plasma pharmacokinetics of butyrate after intravenous administration of sodium butyrate or oral administration of tributyrin or sodium butyrate to mice and rats. Cancer Chemother Pharmacol 43: 445-453.
17. Arbibe L, Kim DW, Batsche E, Pedron T, Mateescu B, et al. (2007) An injected bacterial effector targets chromatin access for transcription factor NF-kappaB to alter transcription of host genes involved in immune responses. Nat Immunol 8: 47-56.
18. Kim DW, Lenzen G, Page AL, Legrain P, Sansonetti PJ, et al. (2005) The Shigella flexneri effector OspG interferes with innate immune responses by targeting ubiquitin-conjugating enzymes. Proc Natl Acad Sci U S A 102: 14046-14051.
19. Li H, Xu H, Zhou Y, Zhang J, Long C, et al. (2007) The phosphothreonine lyase activity of a bacterial type III effector family. Science 315: 1000-1003.
20. Heyman SN, Ginosar Y, Shapiro M, Kluger Y, Marx N, et al. (1997) Diarrheal epidemics among Rwandan refugees in 1994. Management and outcome in a field hospital. J Clin Gastroenterol 25: 595-601.
21. Mitra AK, Engleberg NC, Glass RI, Chowdhury MK, (1990) Fatal dysentery in rural Bangladesh. J Diarrhoeal Dis Res 8: 12-17.
22. Patwari AK, Anand VK, Aneja S, Sharma D, (1995) Persistent diarrhea: management in a diarrhea treatment unit. Indian Pediatr 32: 277-284.
23. Kida Y, Shimizu T, Kuwano K, (2006) Sodium butyrate up-regulates cathelicidin gene expression via activator protein-1 and histone acetylation at the promoter region in a human lung epithelial cell line, EBC-1. Mol Immunol 43: 1972-1981.
24. Schauber J, Iffland K, Frisch S, Kudlich T, Schmausser B, et al. (2004) Histone-deacetylase inhibitors induce the cathelicidin LL-37 in gastrointestinal cells. Mol Immunol 41: 847-854.
25. Schwab M, Reynders V, Shastri Y, Loitsch S, Stein J, et al. (2007) Role of nuclear hormone receptors in butyrate-mediated up-regulation of the antimicrobial peptide cathelicidin in epithelial colorectal cells. Mol Immunol 44: 2107-2114.
26. Termen S, Tollin M, Rodriguez E, Sveinsdottir SH, Johannesson B, et al. (2008) PU.1 and bacterial metabolites regulate the human gene CAMP encoding antimicrobial peptide LL-37 in colon epithelial cells. Mol Immunol 45: 3947-3955.
27. Le Poul E, Loison C, Struyf S, Springael JY, Lannoy V, et al. (2003) Functional characterization of human receptors for short chain fatty acids and their role in polymorphonuclear cell activation. J Biol Chem 278: 25481-25489.
28. Maslowski KM, Vieira AT, Ng A, Kranich J, Sierro F, et al. (2009) Regulation of inflammatory responses by gut microbiota and chemoattractant receptor GPR43. Nature 461: 1282-1286.
29. Raqib R, Ljungdahl A, Lindberg AA, Wretlind B, Andersson U, et al. (1996) Dissociation between cytokine mRNA expression and protein production in shigellosis. Eur J Immunol 26: 1130-1138.
30. Hale TL, Formal SB, (1981) Protein synthesis in HeLa or Henle 407 cells infected with Shigella dysenteriae 1, Shigella flexneri 2a, or Salmonella typhimurium W118. Infect Immun 32: 137-144.
31. Obrig TG, Moran TP, Brown JE, (1987) The mode of action of Shiga toxin on peptide elongation of eukaryotic protein synthesis. Biochem J 244: 287-294.
32. Beutler B, Cerami A, (1989) The biology of cachectin/TNF-a primary mediator of the host response. Annu Rev Immunol 7: 625-655.
33. Carol M, Lambrechts A, Van Gossum A, Libin M, Goldman M, et al. (1998) Spontaneous secretion of interferon gamma and interleukin 4 by human intraepithelial and lamina propria gut lymphocytes. Gut 42: 643-649.
34. Cunnane G, Bjork L, Ulfgren AK, Lindblad S, FitzGerald O, et al. (1999) Quantitative analysis of synovial membrane inflammation: a comparison between automated and conventional microscopic measurements. Ann Rheum Dis 58: 493-499.
35. Su J, Zhang N, Ho PC, (2004) Determination of tributyrin and its metabolite butyrate in Wistar rat plasma samples by gas chromatography/mass spectrometry. Rapid Commun Mass Spectrom 18: 2217-2222.