Using microfluidics for fast, accurate measurement of lysozyme nucleation kinetics

Crystal Growth and Design

Volumn 11, Issue 5, 2011, Pages 1527-1530

  Ildefonso, M ^a,b   Candoni, N ^a,b   Veesler, S ^a,b  

^a CNRS   (France)

^b University of Marseille   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ACCURATE MEASUREMENT; FAST METHODS; NUCLEATION KINETICS; WATER-SOLUBLE MOLECULE;

ENZYMES; NUCLEATION;

MICROFLUIDICS;

EID: 79957935965     PISSN: 15287483     EISSN: 15287505     Source Type: Journal    
DOI: 10.1021/cg101431g     Document Type: Article

References (20)

1. Li, L.; Mustafi, D.; Fu, Q.; Tereshko, V.; Chen, D. L.; Tice, J. D.; Ismagilov, R. F. Nanoliter microfluidic hybrid method for simultaneous screening and optimization validated with crystallization of membrane proteins. Proc. Natl. Acad. Sci. 2006, 103(51), 19243-19248. (Pubitemid 46026018)
2. Shim, J.-U.; Cristobal, G.; Link, D. R.; Thorsen, T.; Fraden, S. Using Microfluidics to Decouple Nucleation and Growth of Protein. Cryst. Growth Des. 2007, 7(11), 2192-2194.
3. Squires, T. M.; Quake, S. R. Microfluidics: Fluid physics at the nanoliter scale. Rev. Mod. Phys. 2005, 77(3), 977.
4. Leng, J.; Salmon, J. B. Microfluidic crystallization. Lab Chip 2009, 9, 24-34.
5. Laval, P.; Salmon, J.-B.; Joanicot, M. A microfluidic device for investigating crystal nucleation kinetics. J. Cryst. Growth 2007, 303, 622-628. (Pubitemid 46617571)
6. Laval, P.; Crombez, A.; Salmon, J.-B. Microfluidic Droplet Method for Nucleation Kinetics Measurements. Langmuir 2009, 25(3), 1836-1841.
7. Ildefonso, M.; Revalor, E.; Punniam, P.; Salmon, J. B.; Candoni, N.; Veesler, S. Nucleation and polymorphism explored via an easy-to-use microfluidic tool. J. Cryst. Growth 2011, article in press.
8. Tsekova, D.; Dimitrova, S.; Nanev, C. N. Heterogeneous nucleation (and adhesion) of lysozyme crystals. J. Cryst. Growth 1999, 196 (2-4), 226-233.
9. Galkin, O.; Vekilov, P. G. Direct determination of the nucleation rates of protein crystals. J. Phys. Chem. B 1999, 103(3), 10965-10971.
10. Revalor, E.; Hammadi, Z.; Astier, J. P.; Grossier, R.; Garcia, E.; Hoff, C.; Furuta, K.; Okutsu, T.; Morin, R.; Veesler, S. Usual and Unusual Crystallization from Solution. J. Cryst. Growth 2010, 312, 939-946.
11. Anna, S. L.; Bontoux, N.; Stone, H. A. Formation of dispersions using "flow focusing" in microchannels. Appl. Phys. Lett. 2003, 82(3), 364-366.
12. Joanicot, M.; Ajdari, A Droplet Control for Microfluidics. Science 2005, 309(5736), 887-888. (Pubitemid 41099914)
13. Laval, P.; Giroux, C.; Leng, J.; Salmon, J.-B. Microfluidic screening of potassium nitrate polymorphism. J. Cryst. Growth 2008, 310(12), 3121-3124.
14. Laval, P.; Lisai, N.; Salmon, J. B.; Joanicot, M. A microfluidic device based on droplet storage for screening solubility diagrams. Lab Chip 2007, 7, 829-834. (Pubitemid 47000812)
15. Selimovic, S.; Jia, Y.; Fraden, S. Measuring the Nucleation Rate of Lysozyme using Microfluidics. Cryst. Growth Des. 2009, 9(4), 1806-1810.
16. Boistelle, R.; Astier, J. P. Crystallization mechanisms in solution. J. Cryst. Growth 1988, 90, 14-30.
17. Cacioppo, E.; Pusey, M. L. The solubility of the tetragonal form of hen egg-white lysozyme from pH 4.0 to 5.4. J. Cryst. Growth 1991, 114, 286-292.
18. Galkin, O.; Vekilov, P. G Are nucleation kinetics of protein crystals similar to those of liquid droplets?. J. Am. Chem. Soc. 2000, 122(1), 156-163. (Pubitemid 30054100)
19. Grossier, R.; Magnaldo, A.; Veesler, S. Ultra-fast Crystallization due to Confinement. J. Cryst. Growth 2010, 312, 487-489.
20. Grossier, R.; Veesler, S. Reaching one single and stable critical cluster through finite sized systems. Cryst. Growth Des. 2009, 9(4), 1917-1922.