Biological in-vivo measurement of dose distribution in patients' lymphocytes by gamma-H2AX immunofluorescence staining: 3D conformal- vs. step-and-shoot IMRT of the prostate gland

Radiation Oncology

Volumn 6, Issue 1, 2011, Pages

  Zwicker, Felix ^a,b   Swartman, Benedict ^a   Sterzing, Florian ^a   Major, Gerald ^a   Weber, Klaus Josef ^a   Huber, Peter E ^a,b   Thieke, Christian ^a,b   Debus, Jürgen ^a   Herfarth, Klaus ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; HISTONE H2AX; H2AFX PROTEIN, HUMAN; HISTONE;

ADULT; AGED; ARTICLE; BODY DISTRIBUTION; CALIBRATION; CLINICAL ARTICLE; COMPUTER ASSISTED RADIOTHERAPY; CONTROLLED STUDY; CORRELATION ANALYSIS; HISTOGRAM; HUMAN; IMMUNOFLUORESCENCE; IN VIVO STUDY; INTENSITY MODULATED RADIATION THERAPY; LYMPHOCYTE; MALE; PH MEASUREMENT; PHYSIOTHERAPY; PROSTATE CANCER; RADIATION DOSE DISTRIBUTION; RADIATION PROTECTION; STAINING; STATISTICAL SIGNIFICANCE; CELL NUCLEUS; FLUORESCENCE MICROSCOPY; LEUKOCYTE; METABOLISM; METHODOLOGY; MIDDLE AGED; PROSTATE TUMOR; RADIATION EXPOSURE; RADIATION RESPONSE; REGRESSION ANALYSIS; THREE DIMENSIONAL IMAGING;

AGED; AGED, 80 AND OVER; CELL NUCLEUS; DOSE-RESPONSE RELATIONSHIP, RADIATION; HISTONES; HUMANS; IMAGING, THREE-DIMENSIONAL; LEUKOCYTES; LYMPHOCYTES; MALE; MICROSCOPY, FLUORESCENCE; MIDDLE AGED; PROSTATIC NEOPLASMS; RADIOTHERAPY, CONFORMAL; REGRESSION ANALYSIS;

EID: 79957934431     PISSN: None     EISSN: 1748717X     Source Type: Journal    
DOI: 10.1186/1748-717X-6-62     Document Type: Article

References (19)

1. Löbrich M, Rief N, Kühne M, Heckmann M, Fleckenstein J, Rübe C, Uder M. In vivo formation and repair of DNA double-strand breaks after computed tomography examinations. Proc Natl Acad Sci USA 2005, 102:8984-8989. 10.1073/pnas.0501895102, 1150277, 15956203.
2. Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem 1998, 273:5858-5868. 2, 10.1074/jbc.273.10.5858, 9488723.
3. Rogakou EP, Boon C, Redon C, Bonner WM. Megabase chromatin domains involved in DNA double-strand breaks in vivo. J Cell Biol 1999, 146:905-916. 3, 10.1083/jcb.146.5.905, 2169482, 10477747.
4. Sak A, Grehl S, Erichsen P, Engelhard M, Grannass A, Levegrün S, Pöttgen C, Groneberg M, Stuschke M. Gamma-H2AX foci formation in peripheral blood lymphocytes of tumor patients after local radiotherapy to different sites of the body: dependence on the dose-distribution, irradiated site and time from start of treatment. Int J Radiat Biol 2007, 83:639-652. 4, 10.1080/09553000701596118, 17729159.
5. Rothkamm K, Löbrich M. Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses. Proc Natl Acad Sci USA 2003, 100:5057-5062. 10.1073/pnas.0830918100, 154297, 12679524.
6. Méndez-Acuña L, Di Tomaso MV, Palitti F, Martínez-López W. Histone post-translational modifications in DNA damage response. Cytogenet Genome Res 2010, 128:28-36. 10.1159/000296275, 20407219.
7. Zwicker F, Ebert M, Huber PE, Debus J, Weber K. A specific inhibitor of protein kinase CK2 delays gamma-H2Ax foci removal and reduces clonogenic survival of irradiated mammalian cells. Radiat Oncol 2011, 6:15. 10.1186/1748-717X-6-15, 3045342, 21310046.
8. Kuefner MA, Grudzenski S, Schwab SA, Azoulay S, Heckmann M, Heinrich MC, Lobrich M, Uder M. X-ray-induced DNA double-strand breaks after angiographic examinations of different anatomic regions. Röfo 2009, 181:374-380.
9. Vickery SR, Cureton KJ, Collins MA. Prediction of body density from skinfolds in black and white young men. Hum Biol 1988, 60:135-149.
10. Kuefner MA, Grudzenski S, Hamann J, Achenbach S, Lell M, Anders K, Schwab SA, Häberle L, Löbrich M, Uder M. Effect of CT scan protocols on x-ray-induced DANN double strand breaks in blood lymphocytes of patients undergoing coronary CT angiography. Eur Radiol 2010, 20:2917-2924. 10.1007/s00330-010-1873-9, 20625737.
11. MacPhail SH, Banáth JP, Yu TY, Chu EHM, Lambur H, Olive PL. Expression of phosphorylated histone H2AX in cultured cell lines following exposure to X-rays. Int J Radiat Biol 2003, 79:351-358. 10.1080/0955300032000093128, 12943243.
12. Banáth JP, Macphail SH, Olive PL. Radiation sensitivity, H2AX phosphorylation, and kinetics of repair of DNA strand breaks in irradiated cervical cancer cell lines. Cancer Res 2004, 64:7144-7149. 10.1158/0008-5472.CAN-04-1433, 15466212.
13. Modesti M, Kanaar R. DNA repair: spot(light)s on chromatin. Curr Biol 2001, 11:229-232.
14. Olive PL, Banáth JP. Phosphorylation of histone H2AX as a measure of radiosensitivity. Int J Radiat Oncol Biol Phys 2004, 58:331-335. 10.1016/j.ijrobp.2003.09.028, 14751500.
15. Olive PL, Banáth JP, Sinnott LT. Phosphorylated histone H2AX in spheroids, tumors, and tissues of mice exposed to etoposide and 3-amino-1,2,4-benzotriazine-1,3-dioxide. Cancer Res 2004, 64:5363-5369. 10.1158/0008-5472.CAN-04-0729, 15289343.
16. Andrievski A, Wilkins RC. The response of gamma-H2AX in human lymphocytes and lymphocytes subsets measured in whole blood cultures. Int J Radiat Biol 2009, 85:369-376. 10.1080/09553000902781147, 19399682.
17. Rothkamm K, Balroop S, Shekhdar J, Fernie P, Goh V. Leukocyte DNA damage after multi-detector row CT: a quantitative biomarker of low-level radiation exposure. Radiology 2007, 242:244-251. 10.1148/radiol.2421060171, 17185671.
18. Bells L, Werbrouck J, Thierens H. Dose response and repair kinetics of gamma-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and gamma-radiation. Int J Radiat Biol 2010, 86(9):760-8. 10.3109/09553002.2010.484479, 20597840.
19. Jucha A, Wegierek-Ciuk A, Koza Z, Lisowska H, Wojcik A, Wojewodzka M, Lankoff A. FociCounter: A freely available PC programme for quantitative and qualitative analysis of gamma-H2AX foci. Mutat Res 2010, 696:16-20.