Structure analysis and site-directed mutagenesis of defined key residues and motives for pilus-related sortase C1 in group B Streptococcus

FASEB Journal

Volumn 25, Issue 6, 2011, Pages 1874-1886

  Cozzi, Roberta ^a   Malito, Enrico ^a,b   Nuccitelli, Annalisa ^a   D'Onofrio, Mariapina ^c   Martinelli, Manuele ^a   Ferlenghi, Ilaria ^a   Grandi, Guido ^a   Telford, John L ^a   Maione, Domenico ^a   Rinaudo, C Daniela ^a  

^a NOVARTIS VACCINES AND DIAGNOSTICS   (Italy)

^b GENOMICS INSTITUTE OF THE NOVARTIS RESEARCH FOUNDATION   (United States)

^c UNIVERSITY OF VERONA   (Italy)

Author keywords

Ancillary proteins; Backbone protein; In vivo complementation mutagenesis; Pilus islands; Pilus polymerization

Indexed keywords

ARGININE DERIVATIVE; CYSTEINE DERIVATIVE; HISTIDINE DERIVATIVE; POLYMER; RECOMBINANT ENZYME; SORTASE; SORTASE C1; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL CELL; BACTERIUM PILUS; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME KINETICS; ENZYME STRUCTURE; GENE MUTATION; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN CLEAVAGE; SITE DIRECTED MUTAGENESIS; STREPTOCOCCUS AGALACTIAE; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINOACYLTRANSFERASES; BACTERIAL PROTEINS; CALCIUM; CRYSTALLOGRAPHY, X-RAY; CYSTEINE ENDOPEPTIDASES; FIMBRIAE, BACTERIAL; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC COMPLEMENTATION TEST; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHYLOGENY; PLASMIDS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN FOLDING; SEQUENCE ALIGNMENT; STREPTOCOCCUS AGALACTIAE;

STREPTOCOCCUS SP. 'GROUP B';

EID: 79957900908     PISSN: 08926638     EISSN: 15306860     Source Type: Journal    
DOI: 10.1096/fj.10-174797     Document Type: Article

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