Molecular and functional characterization of the gilthead seabream β-defensin demonstrate its chemotactic and antimicrobial activity

Molecular Immunology

Volumn 48, Issue 12-13, 2011, Pages 1432-1438

  Cuesta, Alberto ^a   Meseguer, José ^a   Esteban, Maria Ángeles ^a  

^a UNIVERSITY OF MURCIA   (Spain)

Author keywords

Defensin; Antimicrobial peptide; Chemotaxis; Gilthead seabream; Real time PCR; Teleost fish

Indexed keywords

BACTERIAL DNA; BETA DEFENSIN; CPG OLIGODEOXYNUCLEOTIDE; MESSENGER RNA; RECOMBINANT PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ANTIMICROBIAL ACTIVITY; ARTICLE; CHEMOTAXIS; CONTROLLED STUDY; EXPRESSED SEQUENCE TAG; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; LEUKOCYTE; LISTONELLA ANGUILLARUM; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PHOTOBACTERIUM DAMSELAE; PHYLOGENY; PRIORITY JOURNAL; PROTEIN EXPRESSION; TELEOST; VIBRIO HARVEYI;

AMINO ACID SEQUENCE; ANIMALS; ANTI-INFECTIVE AGENTS; BACILLUS SUBTILIS; BASE SEQUENCE; BETA-DEFENSINS; CHEMOTAXIS; FISH PROTEINS; GENE EXPRESSION PROFILING; HEK293 CELLS; HUMANS; LEUKOCYTES; MOLECULAR SEQUENCE DATA; PHOTOBACTERIUM; PHYLOGENY; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SEA BREAM; SEQUENCE ANALYSIS, DNA; VIBRIO;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); LISTONELLA ANGUILLARUM; PHOTOBACTERIUM DAMSELAE; SPARUS AURATA; TELEOSTEI; VIBRIO;

EID: 79957805002     PISSN: 01615890     EISSN: 18729142     Source Type: Journal    
DOI: 10.1016/j.molimm.2011.03.022     Document Type: Article

References (23)

1. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 1990, 215:403-410.
2. Bulet P., Stocklin R., Menin L. Anti-microbial peptides: from invertebrates to vertebrates. Immunol. Rev. 2004, 198:169-184.
3. Casadei E., Wang T., Zou J., Gonzalez-Vecino J.L., Wadsworth S., Secombes C.J. Characterization of three novel beta-defensin antimicrobial peptides in rainbow trout (Oncorhynchus mykiss). Mol. Immunol. 2009, 46:3358-3366.
4. Cuesta A., Dios S., Figueras A., Novoa B., Esteban M.A., Meseguer J., Tafalla C. Identification of six novel CC chemokines in gilthead seabream (Sparus aurata) implicated in the antiviral immune response. Mol. Immunol. 2010, 47:1235-1243.
5. Cuesta A., Esteban M.A., Meseguer J. The expression profile of TLR9 mRNA and CpG ODNs immunostimulatory actions in the teleost gilthead seabream points to a major role of lymphocytes. Cell. Mol. Life Sci. 2008, 65:2091-2104.
6. Cuesta A., Meseguer J., Esteban M.A. The antimicrobial peptide hepcidin exerts an important role in the innate immunity against bacteria in the bony fish gilthead seabream. Mol. Immunol. 2008, 45:2333-2342.
7. Falco A., Chico V., Marroqui L., Perez L., Coll J.M., Estepa A. Expression and antiviral activity of a beta-defensin-like peptide identified in the rainbow trout (Oncorhynchus mykiss) EST sequences. Mol. Immunol. 2008, 45:757-765.
8. Falco A., Brocal I., Perez L., Coll J.M., Estepa A., Tafalla C. In vivo modulation of the rainbow trout (Oncorhynchus mykiss) immune response by the human alpha defensin 1, HNP1. Fish Shellfish Immunol. 2008, 24:102-112.
9. Falco A., Ortega-Villaizan M., Chico V., Brocal I., Perez L., Coll J.M., Estepa A. Antimicrobial peptides as model molecules for the development of novel antiviral agents in aquaculture. Mini Rev. Med. Chem. 2009, 9:1159-1164.
10. Guani-Guerra E., Santos-Mendoza T., Lugo-Reyes S.O., Teran L.M. Antimicrobial peptides: general overview and clinical implications in human health and disease. Clin. Immunol. 2010, 135:1-11.
11. Jin J.Y., Zhou L., Wang Y., Li Z., Zhao J.G., Zhang Q.Y., Gui J.F. Antibacterial and antiviral roles of a fish beta-defensin expressed both in pituitary and testis. PLoS One 2010, 5:e12883.
12. Lai Y., Gallo R.L. AMPed up immunity: how antimicrobial peptides have multiple roles in immune defense. Trends Immunol. 2009, 30:131-141.
13. Nam B.H., Moon J.Y., Kim Y.O., Kong H.J., Kim W.J., Lee S.J., Kim K.K. Multiple beta-defensin isoforms identified in early developmental stages of the teleost Paralichthys olivaceus. Fish Shellfish Immunol. 2010, 28:267-274.
14. Pazgier M., Hoover D.M., Yang D., Lu W., Lubkowski J. Human beta-defensins. Cell. Mol. Life Sci. 2006, 63:1294-1313.
15. Platz J., Beisswenger C., Dalpke A., Koczulla R., Pinkenburg O., Vogelmeier C., Bals R. Microbial DNA induces a host defense reaction of human respiratory epithelial cells. J. Immunol. 2004, 173:1219-1223.
16. Rajanbabu V., Chen J.Y. Applications of antimicrobial peptides from fish and perspectives for the future. Peptides 2010, 32:415-420.
17. Tamura K., Dudley J., Nei M., Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 2007, 24:1596-1599.
18. Thompson J.D., Higgins D.G., Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994, 22:4673-4680.
19. Vora P., Youdim A., Thomas L.S., Fukata M., Tesfay S.Y., Lukasek K., Michelsen K.S., Wada A., Hirayama T., Arditi M., Abreu M.T. Beta-defensin-2 expression is regulated by TLR signaling in intestinal epithelial cells. J. Immunol. 2004, 173:5398-5405.
20. Yang D., Biragyn A., Kwak L.W., Oppenheim J.J. Mammalian defensins in immunity: more than just microbicidal. Trends Immunol. 2002, 23:291-296.
21. Zasloff M. Antimicrobial peptides of multicellular organisms. Nature 2002, 415:389-395.
22. Zhao J.G., Zhou L., Jin J.Y., Zhao Z., Lan J., Zhang Y.B., Zhang Q.Y., Gui J.F. Antimicrobial activity-specific to Gram-negative bacteria and immune modulation-mediated NF-kappaB and Sp1 of a medaka beta-defensin. Dev. Comp. Immunol. 2009, 33:624-637.
23. Zou J., Mercier C., Koussounadis A., Secombes C. Discovery of multiple beta-defensin like homologues in teleost fish. Mol. Immunol. 2007, 44:638-647.