Assessing Blood–Cerebrospinal Fluid Barrier Permeability in the Rat Embryo

Methods in Molecular Biology

Volumn 686, Issue , 2011, Pages 247-265

  Saunders, Norman R ^a   Ek, C Joakim ^a   Habgood, Mark D ^a   Johansson, Pia ^b   Liddelow, Shane ^a   Dziegielewska, Katarzyna M ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b INSTITUTE OF EPIDEMIOLOGY   (Germany)

Author keywords

Blood CSF barrier; Cerebrospinal fluid; Choroid plexus; Development; Rat embryo

Indexed keywords

ADULT; ANIMAL EXPERIMENT; ANIMAL TISSUE; BLOOD CEREBROSPINAL FLUID BARRIER; BLOOD SAMPLING; CEREBROSPINAL FLUID; CHOROID PLEXUS; EMBRYO; MALE; NONHUMAN; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; RAT; RAT EMBRYO; ANIMAL; ANIMAL EMBRYO; ARTICLE; BLOOD BRAIN BARRIER; FEMALE; METABOLISM; PERMEABILITY; PHYSIOLOGY; SPRAGUE DAWLEY RAT; VASCULARIZATION;

ANIMALS; BLOOD-BRAIN BARRIER; CEREBROSPINAL FLUID; EMBRYO, MAMMALIAN; FEMALE; PERMEABILITY; RATS; RATS, SPRAGUE-DAWLEY;

MLCS; MLOWN;

EID: 79957769638     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-938-3_11     Document Type: Chapter

References (18)

1. Johansson PA, Dziegielewska KM, Liddelow SA, Saunders NR (2008) The blood-CSF barrier explained: when development is not immaturity. Bioessays 30:237–48
2. Fossan G, Cavanagh ME, Evans CAN, Malinowska DH, Møllgård K, Reynolds ML, Saunders NR (1985) CSF-brain permeability in the immature sheep fetus: a CSF-brain barrier. Dev Brain Res 18:113–24
3. Møllgård K, Balslev Y, Lauritzen B, Saunders NR (1987) Cell junctions and membrane specializations in the ventricular zone (germinal matrix) of the developing sheep brain: a CSF-brain barrier. J Neurocytol 16:433–44
4. Ek CJ, Habgood MD, Dziegielewska KM, Potter A, Saunders NR (2001) Permeability and route of entry for lipid-insoluble molecules across brain barriers in developing Monodelphis domestica. J Physiol 536:841–53
5. Habgood MD, Sedgwick JE, Dziegielewska KM, Saunders NR (1992) A developmentally regulated blood-cerebrospinal fluid transfer mechanism for albumin in immature rats. J Physiol 456:181–92
6. Davson H, Welch K, Segal MB (1987). Physiology and pathophysiology of the cerebrospinal fluid. Churchill Livingstone, London
7. Liddelow S, Dziegielewska KM, Ek CJ, Johansson PA, Potter A, Saunders NR (2009) Cellular transfer of macromolecules across the developing choroid plexus of Monodelphis domestica. Eur J Neurosci 29:253–66
8. Dziegielewska KM, Evans CAN, Malinowska DH, Møllgård K, Reynolds JM, Reynolds ML, Saunders NR (1979) Studies of the development of brain barrier systems to lipid insoluble molecules in fetal sheep. J Physiol 292:207–31
9. Dziegielewska KM, Habgood MD, Møllgård K, Stagaard M, Saunders NR (1991) Species-specific transfer of plasma albumin from blood into different cerebrospinal fluid compartments in the fetal sheep. J Physiol 439:215–37
10. Stonestreet BS, Patlak CS, Pettigrew KD, Reilly CB, Cserr HF (1996) Ontogeny of blood-brain barrier function in ovine fetuses, lambs, and adults. Am J Physiol 271:R1594–601
11. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
12. Mancini G, Carbonara AO, Heremans JF (1965) Immunochemical quantification of antigens by single radial immunodiffusion. Int J Immunochem 2:235–54
13. Johansson PA, Dziegielewska KM, Ek CJ, Habgood MD, Liddelow SA, Potter AM, Stolp H.B, Saunders NR (2006) Blood-CSF barrier function in the rat embryo. Eur J Neurosci 24:65–76
14. Rawson RA (1943) The binding of T-1824 and structurally related diazo dyes by the plasma proteins. Am J Physiol 138:708–17
15. Dallal MM, Chang S-W (1994) Evans blue dye in the assessment of permeability-surface area product in perfused rat lungs. J Appl Physiol 77:1030–5
16. Butler H, Juurlink BHJ (1987) An atlas for staging mammalian and chick embryos. CRC, Boca Raton
17. Saunders NR (1992) Ontogenic development of brain barrier mechanism. In: Bradbury MWB (ed) Handbook of experimental pharmacology: physiology and pharmacology of the blood-brain barrier. Springer, Berlin, pp 327–369
18. Wakai S, Hirokawa N (1981) Development of blood-cerebrospinal fluid barrier to horseradish peroxidase in the avian choroidal epithelium. Cell Tissue Res 214:271–8