Intracellular degradation of Fusobacterium nucleatum in human gingival epithelial cells

Molecules and Cells

Volumn 30, Issue 6, 2010, Pages 519-526

  Ji, Suk ^b   Shin, Ji Eun ^a   Kim, Yong Cheol ^a   Choi, Youngnim ^a  

^a Seoul National University   (South Korea)

^b Korea University College of Medicine   (South Korea)

Author keywords

Endosomes; Fusobacterium nucleatum; Gingival epithelium; Invasion; Lysosomes

Indexed keywords

AMOXICILLIN; BETA DEFENSIN 2; BETA DEFENSIN 3; GENTAMICIN; ANTIINFECTIVE AGENT; BETA DEFENSIN;

ARTICLE; BIODEGRADATION; CELL IMMORTALIZATION; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CYTOPATHOGENIC EFFECT; ENDOSOME; EPITHELIUM CELL; FLOW CYTOMETRY; FUSOBACTERIUM NUCLEATUM; GINGIVA; HOST CELL; HUMAN; HUMAN CELL; KERATINOCYTE; LYSOSOME; PATHOGENESIS; PERIODONTITIS; PORPHYROMONAS GINGIVALIS; PROTEIN ANALYSIS; PROTEIN LOCALIZATION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STREPTOCOCCUS GORDONII; STREPTOCOCCUS SANGUINIS; TRANSMISSION ELECTRON MICROSCOPY; CELL LINE; CYTOLOGY; ELECTRON TOMOGRAPHY; IMMUNOLOGY; METABOLISM; METHODOLOGY; MICROBIOLOGY; PATHOLOGY; ULTRASTRUCTURE;

BACTERIA (MICROORGANISMS); FUSOBACTERIUM NUCLEATUM; PORPHYROMONAS GINGIVALIS;

ANTI-INFECTIVE AGENTS; BETA-DEFENSINS; CELL LINE; ELECTRON MICROSCOPE TOMOGRAPHY; EPITHELIAL CELLS; FLOW CYTOMETRY; FUSOBACTERIUM NUCLEATUM; GINGIVA; HUMANS; MICROSCOPY, CONFOCAL; PERIODONTITIS;

EID: 79957733056     PISSN: 10168478     EISSN: 02191032     Source Type: Journal    
DOI: 10.1007/s10059-010-0142-8     Document Type: Article

References (32)

1. Allenspach-Petrzilka, G. E., and Guggenheim, B. (1983). Bacterial invasion of the periodontium; an important factor in the pathogenesis of periodontitis? J. Clin. Periodontol. 10, 609-617. (Pubitemid 14202181)
2. Alonso, A., and García-del Portillo, F. (2004). Hijacking of eukaryotic functions by intracellular bacterial pathogens. Int. Microbiol. 7, 181-191. (Pubitemid 40515130)
3. Amano, A., Nakagawa, L., and Yoshimori, T. (2006). Autophagy in innate immunity against intracellular bacteria. J. Biochem. 140, 161-166. (Pubitemid 44618901)
4. Bachrach, G., Rosen, G., Bellalou, M., Naor, R., and Sela, M. N. (2004). Identification of a Fusobacterium nucleatum 65 kDa serine protease. Oral Microbiol. Immunol. 19, 155-159. (Pubitemid 38649608)
5. Bauer, C., Schoonbroodt, D., Wagner, C., and Horsmans, Y. (2000). Liver abscesses due to Fusobacterium species. Liver 20, 267-268. (Pubitemid 30342728)
6. Chaim, W., and Mazor, M. (1992). Intraamniotic infection with fusobacteria. Arch. Gynecol. Obstet. 251, 1-7.
7. Chung, W. O., and Dale, B. A. (2004). Innate immune response of oral and foreskin keratinocytes: utilization of different signaling pathways by various bacterial species. Infect. Immun. 72, 352-358. (Pubitemid 38018195)
8. Feng, Z., and Weinberg, A. (2006). Role of bacteria in health and disease of periodontal tissues. Periodontol. 2000 40, 50-76. (Pubitemid 43434241)
9. Fuller, J. R., Craven, R. R., Hall, J. D., Kijek, T. M., Taft-Benz, S., and Kawula, T. H. (2008). RipA, a cytoplasmic membrane protein conserved among Francisella species, is required for intracellular survival. Infec. Immun. 76, 4934-4943.
10. Grabenstein, J. P., Marceau, M., Pujol, C., Simonet, M., and Bliska, J. B. (2004). The response regulator PhoP of Yersinia pseudotuberculosis is important for replication in macrophages and for virulence. Infect. Immun. 72, 4973-4984. (Pubitemid 39223380)
11. Gursoy, U. K., Könönen, E., and Uitto, V. J. (2008). Intracellular replication of fusobacteria requires new actin filament formation of epithelial cells. APMIS 116, 1063-1070.
12. Han, Y. W., Shi, W., Huang, G. T., Kinder Haake, S., Park, N. H., Kuramitsu, H., and Genco, R. J. (2000). Interactions between periodontal bacteria and human oral epithelial cells: Fusobacterium nucleatum adheres to and invades epithelial cells. Infect. Immun. 68, 3140-3146. (Pubitemid 30340401)
13. Han, Y. W., Redline, R. W., Li, M., Yin, L., Hill, G. B., and McCormick, T. S. (2004). Fusobacterium nucleatum induces premature and term stillbirths in pregnant mice: implication of oral bacteria in preterm birth. Infect. Immun. 72, 2272-2279. (Pubitemid 38419943)
14. Hanada, H., Moriyama, Y., Maeda, M., and Futai, M. (1999). Kinetic studies of chromaffin granule H+-ATPase and effects of bafilomycin A1. Biochem. Biophys. Res. Commun. 170, 873-878.
15. Hintermann, E., Haake, S. K., Christen, U., Sharabi, A., and Quaranta, V. (2002). Discrete proteolysis of focal contact and adherens junction components in Porphyromonas gingivalis-infected oral keratinocytes: a strategy for cell adhesion and migration disabling. Infect. Immun. 70, 5846-5856.
16. Hsu, C. Y., and Luh, K. T. (1995). Cytology of pulmonary Fusobacterium nucleatum infection. A case report. Acta Cytol. 39, 114-117.
17. Hultén, K., Rigo, R., Gustafsson, I., and Engstrand, L. (1996). New pharmacokinetic in vitro model for studies of antibiotic activity against intracellular microorganisms. Antimicrob. Agents Chemother. 40, 2727-2731. (Pubitemid 26404333)
18. Ji, S., Hyun, J., Park, E., Lee, B. L., Kim, K. K., and Choi, Y. (2007a). Susceptibility of various oral bacteria to antimicrobial peptides and to phagocytosis by neutrophils. J. Periodont. Res. 42, 410-419.
19. Ji, S., Kim, Y., Min, B.-Y., Han, S. H., and Choi, Y. (2007b). Innate immune responses of gingival epithelial cells to nonperiodontopathic and periodontopathic bacteria. J. Periodont. Res. 42, 503-510.
20. Ji, S., Shin, J. E., Kim, Y. S., Oh, J. E., Min, B. M., and Choi, Y. (2009). Toll-like receptor 2 and NALP2 mediate induction of human beta-defensins by Fusobacterium nucleatum in gingival epithelial cells. Infect. Immun. 77, 1044-1052.
21. Kim, Y. C., Ko, Y., Hong, S.-D., Kim, K. Y., Lee, Y. H., Chae, C., and Choi, Y. (2010). Presence of Porphyromonas gingivalis and plasma cell dominance in gingival tissues with periodontitis. Oral Dis. 16, 375-381.
22. Krisanaprakornkit, S., Kimball, J. R., Weinberg, A., Darveau, R. P., Bainbridge, B. W., and Dale, B. A. (2000). Inducible expression of human beta-defensin 2 by Fusobacterium nucleatum in oral epithelial cells: multiple signaling pathways and role of commensal bacteria in innate immunity and the epithelial barrier. Infect. Immun. 68, 2907-2915. (Pubitemid 30253871)
23. Lamont, R. J., Chan, A., Belton, C. M., Izutsu, K. T., Vasel, D., and Weinberg, A. (1995). Porphyromonas gingivalis invasion of gingival epithelial cells. Infect. Immun. 63, 3878-3885.
24. Lux, R., Miller, J. N., Park, N. H., and Shi, W. (2001). Motility and chemotaxis in tissue penetration of oral epithelial cell layers by Treponema denticola. Infect. Immun. 69, 6276-6283. (Pubitemid 32885192)
25. Meyer, D. H., Lippmann, J. E., and Fives-Taylor, P. M. (1996). Invasion of epithelial cells by Actinobacillus actinomycetemcomitans: a dynamic, multistep process. Infect. Immun. 64, 2988-2997. (Pubitemid 26256137)
26. Park, N.-H., Min, B.-M., Li, S. L., Huang, M. Z., Cherick, H. M., and Doniger, J. (1991). Immortalization of normal human oral keratinocytes with type 16 human papillomavirus. Carcinogenesis 12, 1627-1631.
27. Pils, S., Schmitter, T., Neske, F., and Hauck, C. R. (2006). Quantification of bacterial invasion into adherent cells by flow cytometry. J. Microbiol. Methods 65, 301-310.
28. Rossano, F., Rizzo, A., Sangnes, M. R., Cipollaro de L'Ero, G., and Tufano, M. A. (1993). Human monocytes and gingival fibroblasts release tumor necrosis factor-a, interleukin-1a and interleukin-6 in response to particulate and soluble fractions of Prevotella melaninogenica and Fusobacterium nucleatum. Int. J. Clin. Res. 23, 165-168.
29. Saito, A., Inagaki, S., and Ishihara, K. (2009). Differential ability of periodontopathic bacteria to modulate invasion of human gingival epithelial cells by Porphyromonas gingivalis. Microb. Pathog. 47, 329-333.
30. Socransky, S. S., Haffajee, A. D., Cugini, M. A., Smith, C., and Kent, R. L. Jr. (1998). Microbial complexes in subgingival plaque. J. Clin. Periodontol. 25, 134-144. (Pubitemid 128705944)
31. Tricker, E., and Cheng, G. (2008). With a little help from my friends: modulation of phagocytosis through TLR activation. Cell Res. 18, 711-712. (Pubitemid 351948233)
32. Yilmaz, O., Verbeke, P., Lamont, R. J., and Ojcius, D. M. (2006). Intercellular spreading of Porphyromonas gingivalis infection in primary gingival epithelial cells. Infect. Immun. 74, 703-710. (Pubitemid 43023126)