One-step simple assay to determine antigen-specific cytotoxic activities by single-color flow cytometry

Biomedical Research

Volumn 32, Issue 2, 2011, Pages 159-166

  Nakagawa, Yohko ^a   Watari, Eiji ^a   Shimizu, Masumi ^a   Takahashi, Hidemi ^a  

^a NIPPON MEDICAL SCHOOL   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; CHROMIUM 51; EDETIC ACID; EPITOPE; PEPTIDE; TRYPSIN;

ACCURACY; AMINO ACID SUBSTITUTION; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIGEN SPECIFICITY; ARTICLE; CD8+ T LYMPHOCYTE; CELL ADHESION; CELL ASSAY; CELL MEDIATED CYTOTOXICITY; CHROMIUM 51 RELEASE; CONTROLLED STUDY; CYTOTOXIC T LYMPHOCYTE; FEMALE; FLOW CYTOMETRY; FLUORESCENCE ANALYSIS; HISTOGRAM; IN VITRO STUDY; INTERMETHOD COMPARISON; LYMPHOCYTE COUNT; LYMPHOCYTE FUNCTION; MOUSE; NONHUMAN; SENSITIVITY ANALYSIS; TUMOR CELL;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; CELL LINE, TUMOR; CELL SEPARATION; CHROMIUM RADIOISOTOPES; COLOR; CYTOTOXICITY TESTS, IMMUNOLOGIC; CYTOTOXICITY, IMMUNOLOGIC; FEMALE; FLOW CYTOMETRY; FLUORESCEINS; FLUORESCENT DYES; MICE; MICE, INBRED BALB C; MICE, TRANSGENIC; MOLECULAR SEQUENCE DATA; SENSITIVITY AND SPECIFICITY; SUCCINIMIDES; T-LYMPHOCYTES, CYTOTOXIC;

EID: 79957687752     PISSN: 03886107     EISSN: 1880313X     Source Type: Journal    
DOI: 10.2220/biomedres.32.159     Document Type: Article

References (17)

1. Barber DL, Wherry EJ and Ahmed R (2003) Cutting edge: rapid in vivo killing by memory CD8 T cells. J Immunol 171, 27-31.
2. + T cells. J Immunol 171, 17-21.
3. Chang L, Gusewitch GA, Chritton DB, Folz JC, Lebeck LK and Nehlsen-Cannarella SL (1993) Rapid flow cytometric assay for the assessment of natural killer cell activity. J Immu-nol Methods 166, 45-54.
4. Flieger D, Gruber R, Schlimok G, Reiter C, Pantel K and Riethmuller G (1995) A novel non-radioactive cellular cytotoxicity test based on the differential assessment of living and killed target and effector cells. J Immunol Methods 180, 1-13.
5. Godoy-Ramirez K, Makitalo B, Thorstensson R, Sandstrom E, Biberfeld G and Gaines H (2005) A novel assay for assessment of HIV-specific cytotoxicity by multiparameter flow cy-tometry. Cytometry A 68, 71-80.
6. Jedema I, van der Werff NM, Barge RM, Willemze R andFalkenburg JH (2004) New CFSE-based assay to determine susceptibility to lysis by cytotoxic T cells of leukemic precursor cells within aheterogeneous target cell population. Blood 103, 2677-2682.
7. Karawajew L, Jung G, Wolf H, Micheel B and Ganzel K (1994) A flow cytometric longterm cytotoxicity assay. J Immunol Methods 177, 119-130.
8. Lecoeur H, Fevrier M, Garcia S, Riviere Y and Gougeon ML (2001) A novel flow cytometric assay for quantitation and multiparametric characterization of cell-mediated cytotoxicity. J Immunol Methods 253, 177-187.
9. Li X, Dancausse H, Grijalva I, Oliveira M and Levi AD (2003) Labeling Schwann cells with CFSE-an in vitro and in vivoztudy. J Neurosci Methods 125, 83-91.
10. McGinnes K, Chapman G, Marks R and Penny R (1986) A fluorescence NK assay using flow cytometry. J Immunol ethods 86, 7-15.
11. Papadopoulos NG, Dedoussis GV, Spanakos G, Gritzapis AD, Baxevanis CN and Papamichail M (1994) An improved fluorescence assay for the determination of lymphocyte-mediated cytotoxicity using flow cytometry. J Immunol Meth-ods 177, 101-111.
12. Takahashi H, Cohen J, Hosmalin A, Cease KB, Houghten R, Cornette JL, DeLisi C, Moss B, Germain RN and Berzofsky JA (1988) An immunodominant epitope of the human immunodeficiency virus envelope glycoprotein gp160 recognized by class I major histocompatibility complex molecule-restricted murine cytotoxic T lymphocytes. Proc Natl Acad Sci U S A 85, 3105-3109.
13. Takahashi H, Houghten R, Putney SD, Margulies DH, Moss B, Germain RN and Berzofsky JA (1989) Structural requirements for class I MHC molecule-mediated antigen presentation and cytotoxic T cell recognition of an immunodominant determinant of the human immunodeficiency virus envelope protein. J Exp Med 170, 2023-2035.
14. Takahashi H, Merli S, Putney SD, Houghten R, Moss B, Germain RN and Berzofsky JA (1989) A single amino acid interchange yields reciprocal CTL specificities for HIV-1 gp160. Science 246, 118-121.
15. Takahashi H, Nakagawa Y, Pendleton CD, Houghten RA, Yokomuro K, Germain RN and Berzofsky JA (1992) Induc-tion of broadly cross-reactive cytotoxic T cells recognizing an HIV-1 envelope determinant. Science 255, 333-336.
16. Takeshita T, Takahashi H, Kozlowski S, Ahlers JD, Pendleton CD, Moore RL, Nakagawa Y, Yokomuro K, Fox BS, Margulies DH and Berzofsky JA (1995) Molecular analysis of the same HIV peptide functionally binding to both a class I and a class II MHC molecule. J Immunol 154, 1973-1986.
17. Yokosuka T, Takase K, Suzuki M, Nakagawa Y, Taki S, Takahashi H, Fujisawa T, Arase H and Saito T (2002) Predominant role of T cell receptor (TCR)-alpha chain in forming preimmune TCR repertoire revealed by clonal TCR reconstitution system. J Exp Med 195, 991-1001.