Alanine-scanning mutagenesis of selected residues in the n-terminal region alters the functionality of LuxO: Lessons from a natural variant LuxOPl91

Journal of Medical Microbiology

Volumn 60, Issue 6, 2011, Pages 856-860

  Khatri, Neelam ^a   Dureja, Chetna ^a   Raychaudhuri, Saumya ^a  

^a INSTITUTE OF MICROBIAL TECHNOLOGY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; BACTERIAL PROTEIN; PROTEIN LUXO; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; CELL DENSITY; GENE EXPRESSION; GENETIC TRANSCRIPTION; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; QUORUM SENSING; TRANSCRIPTION INITIATION; VIBRIO CHOLERAE;

ALANINE; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; REPRESSOR PROTEINS; VIBRIO CHOLERAE;

EID: 79957609275     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.022988-0     Document Type: Article

References (10)

1. Bassler, B. L., Wright, M. & Silverman, M. R. (1994). Sequence and function of LuxO, a negative regulator of luminescence in Vibrio harveyi. Mol Microbiol 12, 403-412.
2. Dongre, M., Tripathi, R., Jain, V. & Raychaudhuri, S. (2008). Functional independence of a variant LuxOPL91 from a non-O1 non-O139 Vibrio cholerae over the activity of CsrA and Fis. J Med Microbiol 57, 1041-1045.
3. Freeman, J. A. & Bassler, B. L. (1999). A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. Mol Microbiol 31, 665-677.
4. Gendlina, I., Gutman, D. M., Thomas, V. & Collins, C. M. (2002). Urea-dependent signal transduction by the virulence regulator UreR. J Biol Chem 277, 37349-37358.
5. Lefevre, F., Remy, M.-H. & Masson, J.-M. (1997). Alanine-stretch scanning mutagenesis: a simple and efficient method to probe protein structure and function. Nucleic Acids Res 25, 447-448.
6. Lenz, D. H. & Bassler, B. L. (2007). The small nucleoid protein Fish is involved in Vibrio cholerae quorum sensing. Mol Microbiol 63, 859-871.
7. Lilley, B. N. & Bassler, B. L. (2000). Regulation of quorum sensing in Vibrio harveyi by LuxO and Sigma-54. Mol Microbiol 36, 940-954.
8. Raychaudhuri, S., Jain, V. & Dongre, M. (2006). Identification of a constitutively active variant of LuxO that affects production of HA/protease and biofilm development in a non-O1, non-O139 Vibrio cholerae O110. Gene 369, 126-133.
9. Vance, R. E., Zhu, J. & Mekalanos, J. J. (2003). A constitutively active variant of the quorum-sensing regulator LuxO affects protease production and biofilm formation in Vibrio cholerae. Infect Immun 71, 2571-2576.
10. Yant, S. R., Park, J., Huang, Y., Mikkelsen, J. G. & Kay, M. A. (2004). Mutational analysis of the N-terminal DNA-binding domain of sleeping beauty transposase: critical residues for DNA binding and hyperactivity in mammalian cells. Mol Cell Biol 24, 9239-9247.