Development of a recombinant adenovirus vector production system free of replication-competent adenovirus by utilizing a packaging size limit of the viral genome

Virus Research

Volumn 158, Issue 1-2, 2011, Pages 154-160

  Suzuki, Takayuki ^a,b   Sasaki, Tomomi ^a   Yano, Koyori ^a   Sakurai, Fuminori ^a,b   Kawabata, Kenji ^a,b   Kondoh, Masuo ^b   Hayakawa, Takao ^c,d   Yagi, Kiyohito ^b   Mizuguchi, Hiroyuki ^a,b  

^a NATIONAL INSTITUTE OF BIOMEDICAL INNOVATION   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c PHARMACEUTICALS AND MEDICAL DEVICES AGENCY   (Japan)

^d KINKI UNIVERSITY   (Japan)

Author keywords

Adenovirus vector; Gene therapy; Packaging size limit; RCA

Indexed keywords

ADENOVIRUS VECTOR; VIRUS DNA;

ADENOVIRUS; ARTICLE; CELL STRAIN; CONTROLLED STUDY; DNA PACKAGING; GENE CASSETTE; GENE INSERTION; GENE LOCUS; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; REPLICATION COMPETENT ADENOVIRUS; REPRODUCIBILITY; TECHNIQUE; TRANSGENE; VIRUS GENOME; VIRUS RECOMBINANT; VIRUS REPLICATION; WILD TYPE;

ADENOVIRIDAE; DNA, VIRAL; GENE DELETION; GENE THERAPY; GENETIC VECTORS; GENETICS, MICROBIAL; GENOME, VIRAL; HUMANS; TRANSGENES; VIROLOGY; VIRUS ASSEMBLY;

ADENOVIRIDAE;

EID: 79956320161     PISSN: 01681702     EISSN: 18727492     Source Type: Journal    
DOI: 10.1016/j.virusres.2011.03.026     Document Type: Article

References (28)

1. Bett A.J., Prevec L., Graham F.L. Packaging capacity and stability of human adenovirus type 5 vectors. J. Virol. 1993, 67(10):5911-5921.
2. Fallaux F.J., Bout A., van der Velde I., van den Wollenberg D.J., Hehir K.M., Keegan J., Auger C., Cramer S.J., van Ormondt H., van der Eb A.J., Valerio D., Hoeben R.C. New helper cells and matched early region 1-deleted adenovirus vectors prevent generation of replication-competent adenoviruses. Hum. Gene Ther. 1998, 9(13):1909-1917.
3. Farson D., Tao L., Ko D., Li Q., Brignetti D., Segawa K., Mittelstaedt D., Harding T., Yu D.C., Li Y. Development of novel E1-complementary cells for adenoviral production free of replication-competent adenovirus. Mol. Ther. 2006, 14(2):305-311.
4. Furcinitti P.S., van Oostrum J., Burnett R.M. Adenovirus polypeptide IX revealed as capsid cement by difference images from electron microscopy and crystallography. EMBO J. 1989, 8(12):3563-3570.
5. Ghosh-Choudhury G., Haj-Ahmad Y., Graham F.L. Protein IX, a minor component of the human adenovirus capsid, is essential for the packaging of full length genomes. EMBO J. 1987, 6(6):1733-1739.
6. Graham F.L., Smiley J., Russell W.C., Nairn R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 1977, 36(1):59-74.
7. Hehir K.M., Armentano D., Cardoza L.M., Choquette T.L., Berthelette P.B., White G.A., Couture L.A., Everton M.B., Keegan J., Martin J.M., Pratt D.A., Smith M.P., Smith A.E., Wadsworth S.C. Molecular characterization of replication-competent variants of adenovirus vectors and genome modifications to prevent their occurrence. J. Virol. 1996, 70(12):8459-8467.
8. Hermens W.T., Verhaagen J. Adenoviral vector-mediated gene expression in the nervous system of immunocompetent Wistar and T cell-deficient nude rats: preferential survival of transduced astroglial cells in nude rats. Hum. Gene Ther. 1997, 8(9):1049-1063.
9. Imler J.L., Bout A., Dreyer D., Dieterle A., Schultz H., Valerio D., Mehtali M., Pavirani A. Trans-complementation of E1-deleted adenovirus: a new vector to reduce the possibility of codissemination of wild-type and recombinant adenoviruses. Hum. Gene Ther. 1995, 6(6):711-721.
10. Ishii-Watabe A., Uchida E., Iwata A., Nagata R., Satoh K., Fan K., Murata M., Mizuguchi H., Kawasaki N., Kawanishi T., Yamaguchi T., Hayakawa T. Detection of replication-competent adenoviruses spiked into recombinant adenovirus vector products by infectivity PCR. Mol. Ther. 2003, 8(6):1009-1016.
11. Koizumi N., Mizuguchi H., Utoguchi N., Watanabe Y., Hayakawa T. Generation of fiber-modified adenovirus vectors containing heterologous peptides in both the HI loop and C terminus of the fiber knob. J. Gene Med. 2003, 5(4):267-276.
12. Lieber A., Steinwaerder D.S., Carlson C.A., Kay M.A. Integrating adenovirus-adeno-associated virus hybrid vectors devoid of all viral genes. J. Virol. 1999, 73(11):9314-9324.
13. Lochmuller H., Jani A., Huard J., Prescott S., Simoneau M., Massie B., Karpati G., Acsadi G. Emergence of early region 1-containing replication-competent adenovirus in stocks of replication-defective adenovirus recombinants (delta E1+delta E3) during multiple passages in 293 cells. Hum. Gene Ther. 1994, 5(12):1485-1491.
14. Louis N., Evelegh C., Graham F.L. Cloning and sequencing of the cellular-viral junctions from the human adenovirus type 5 transformed 293 cell line. Virology 1997, 233(2):423-429.
15. Maizel J.V., White D.O., Scharff M.D. The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology 1968, 36(1):115-125.
16. Mizuguchi H., Kay M.A. Efficient construction of a recombinant adenovirus vector by an improved in vitro ligation method. Hum. Gene Ther. 1998, 9(17):2577-2583.
17. Mizuguchi H., Kay M.A. A simple method for constructing E1- and E1/E4-deleted recombinant adenoviral vectors. Hum. Gene Ther. 1999, 10(12):2013-2017.
18. Mizuguchi H., Koizumi N., Hosono T., Utoguchi N., Watanabe Y., Kay M.A., Hayakawa T. A simplified system for constructing recombinant adenoviral vectors containing heterologous peptides in the HI loop of their fiber knob. Gene Ther. 2001, 8(9):730-735.
19. Murakami P., Pungor E., Files J., Do L., van Rijnsoever R., Vogels R., Bout A., McCaman M. A single short stretch of homology between adenoviral vector and packaging cell line can give rise to cytopathic effect-inducing, helper-dependent E1-positive particles. Hum. Gene Ther. 2002, 13(8):909-920.
20. Murakami P., Havenga M., Fawaz F., Vogels R., Marzio G., Pungor E., Files J., Do L., Goudsmit J., McCaman M. Common structure of rare replication-deficient E1-positive particles in adenoviral vector batches. J. Virol. 2004, 78(12):6200-6208.
21. Parks R.J., Chen L., Anton M., Sankar U., Rudnicki M.A., Graham F.L. A helper-dependent adenovirus vector system: removal of helper virus by Cre-mediated excision of the viral packaging signal. Proc. Natl. Acad. Sci. U.S.A. 1996, 93(24):13565-13570.
22. Robert J.J., Gauffeny I., Maccario J., Jullien C., Benoit P., Vigne E., Crouzet J., Perricaudet M., Yeh P. Degenerated pIX-IVa2 adenoviral vector sequences lowers reacquisition of the E1 genes during virus amplification in 293 cells. Gene Ther. 2001, 8(22):1713-1720.
23. Sakurai F., Kawabata K., Yamaguchi T., Hayakawa T., Mizuguchi H. Optimization of adenovirus serotype 35 vectors for efficient transduction in human hematopoietic progenitors: comparison of promoter activities. Gene Ther. 2005, 12(19):1424-1433.
24. Sandig V., Youil R., Bett A.J., Franlin L.L., Oshima M., Maione D., Wang F., Metzker M.L., Savino R., Caskey C.T. Optimization of the helper-dependent adenovirus system for production and potency in vivo. Proc. Natl. Acad. Sci. U.S.A. 2000, 97(3):1002-1007.
25. Tollefson A.E., Ryerse J.S., Scaria A., Hermiston T.W., Wold W.S. The E3-11.6-kDa adenovirus death protein (ADP) is required for efficient cell death: characterization of cells infected with adp mutants. Virology 1996, 220(1):152-162.
26. Tollefson A.E., Scaria A., Hermiston T.W., Ryerse J.S., Wold L.J., Wold W.S. The adenovirus death protein (E3-11.6K) is required at very late stages of infection for efficient cell lysis and release of adenovirus from infected cells. J. Virol. 1996, 70(4):2296-2306.
27. Xu Q., Arevalo M.T., Pichichero M.E., Zeng M. A new complementing cell line for replication-incompetent E1-deleted adenovirus propagation. Cytotechnology 2006, 51(3):133-140.
28. Zhu J., Grace M., Casale J., Chang A.T., Musco M.L., Bordens R., Greenberg R., Schaefer E., Indelicato S.R. Characterization of replication-competent adenovirus isolates from large-scale production of a recombinant adenoviral vector. Hum. Gene Ther. 1999, 10(1):113-121.