Isolation and characterization of virulent Aeromonas veronii from ascitic fluid of oscar Astronotus ocellatus showing signs of infectious dropsy

Diseases of Aquatic Organisms

Volumn 94, Issue 1, 2011, Pages 29-39

  Sreedharan, K ^a   Philip, R ^a   Singh, I S Bright ^a  

^a Department of Physics   (India)

Author keywords

Aeromonas veronii; Astronotus ocellatus; Ornamental fishes; Oscar; Virulence

Indexed keywords

ANTIINFECTIVE AGENT;

ANTIBIOTICS; BACTERIAL DISEASE; BACTERIUM; BIOCHEMICAL COMPOSITION; COLOR; COMMERCIAL SPECIES; ENZYME ACTIVITY; FECUNDITY; GENE EXPRESSION; GENETIC ANALYSIS; IDENTIFICATION METHOD; INFECTIOUS DISEASE; MATURATION; ORNAMENTAL SPECIES; PATHOGENICITY; PERCIFORM; TOXICITY; VIRULENCE;

AEROMONAS; ANIMAL; ANIMAL DISEASE; ANTIBIOTIC RESISTANCE; ARTICLE; ASCITES FLUID; CICHLID; EDEMA; FISH DISEASE; GENE EXPRESSION REGULATION; GENETICS; GRAM NEGATIVE INFECTION; ISOLATION AND PURIFICATION; MICROBIOLOGY; PATHOGENICITY; PHYLOGENY; VIRULENCE;

AEROMONAS; ANIMALS; ANTI-BACTERIAL AGENTS; ASCITIC FLUID; CICHLIDS; DRUG RESISTANCE, BACTERIAL; EDEMA; FISH DISEASES; GENE EXPRESSION REGULATION, BACTERIAL; GRAM-NEGATIVE BACTERIAL INFECTIONS; PHYLOGENY; VIRULENCE;

AEROMONAS VERONII; ASTRONOTUS OCELLATUS; CARASSIUS AURATUS; CICHLIDAE; PISCES;

EID: 79956309283     PISSN: 01775103     EISSN: 16161580     Source Type: Journal    
DOI: 10.3354/dao02304     Document Type: Article

References (90)

1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403-410
2. Altwegg M, Steigerwalt AG, Altwegg-Bissig R, Luthy-Hottenstein J, Brenner DJ (1990) Biochemical identification of Aeromonas genospecies isolated from humans. J Clin Microbiol 28:258-264 (Pubitemid 20050932)
3. APHA (1995) Standard methods for the examination of water and wastewater, 19th edn. American Public Health Association, American Water Works Association, and Water Pollution Control Federation, Washington, DC
4. Bauer AW, Kirby WMM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol 45:493-496
5. Beeching SC (1995) Colour pattern and inhibition of aggression in the cichlid fish Astronotus ocellatus. J Fish Biol 47:50-58 (Pubitemid 26451019)
6. Biscardi D, Castaldo A, Gualilo O, de Fusco R (2002) The occurrence of cytotoxic Aeromonas hydrophila strains in Italian mineral and thermal waters. Sci Total Environ 292: 255-263 (Pubitemid 34606028)
7. Burr SE, Frey J (2007) Analysis of type III effector genes in typical and atypical Aeromonas salmonicida. J Fish Dis 30: 711-714 (Pubitemid 47624002)
8. Burr SE, Pugovkin D, Wahli T, Segner H, Frey J (2005) Attenuated virulence of an Aeromonas salmonicida subsp. salmonicida type III secretion mutant in a rainbow trout model. Microbiology 151:2111-2118 (Pubitemid 40909052)
9. Carnahan AM, Altwegg M (1996) Taxonomy. In: Austin B, Altwegg M, Gosling PJ, Joseph S (eds) The genus Aeromonas. Wiley, New York, NY, p 1-38
10. Carnahan A, Hammontree L, Bourgeois L, Joseph SW (1990) Pyrazinamidase activity as a phenotypic marker for several Aeromonas spp. isolated from clinical specimens. J Clin Microbiol 28:391-392 (Pubitemid 20050964)
11. Carnahan AM, Behram S, Joseph SW (1991) Aerokey II: a flexible key for identifying clinical Aeromonas species. J Clin Microbiol 29:2843-2849
12. Chacon MR, Soler L, Groisman EA, Guarro J, Figueras MJ (2004) Type III secretion system genes in clinical Aeromonas isolates. J Clin Microbiol 42:1285-1287 (Pubitemid 38332868)
13. Chopra AK, Xu XJ, Ribardo D, Gonzalez M, Kuhl K, Petersojn JW, Houston CW (2000) The cytotoxic enterotoxin of Aeromonas hydrophila induces proinflammatory cytokine production and activates arachidonic acid metabolism in macrophages. Infect Immun 68:2808-2818 (Pubitemid 30253860)
14. Collee JG, Fraser AG, Marmion BP, Simmons A (1996) Practical medical microbiology. Churchill Livingstone, New York, NY
15. Epple HJ, Mankertz J, Ignatius R, Liesenfeld O, Fromm M, Zeitz S, Chakraborty T, Schulzke JD (2004) Aeromonas hydrophila beta-hemolysin induces active chloride secretion in colon epithelial cells (HT-29/B6). Infect Immun 72:4848-4858 (Pubitemid 38989345)
16. Ewing WH (1986) Edwards and Ewing's identification of Enterobacteriaceae, 4th edn. Elsevier Science Publishing, New York, NY
17. FAO (Food and Agricultural Organization of the United Nations) (2006) State of the world aquaculture. FAO Fish Tech Pap 500. FAO, Rome
18. Fehr D, Burr SE, GilbertM, Alayer J, Frey J, Popoff MR (2007) Aeromonas exoenzyme T of Aeromonas salmonicida is a bifunctional protein that targets the host cytoskeleton. J Biol Chem 282:28843-28852 (Pubitemid 47606029)
19. Filler G, Ehrich JH, Strauch E, Beutin L (2000) Acute renal failure in an infant associated with cytotoxic Aeromonas sobria isolated from patient's stool and from aquarium water as suspected source of infection. J Clin Microbiol 38:469-470 (Pubitemid 30043351)
20. Freeman DJ, Elakliner FR, Keane CT (1989) New method for detecting slime production by coagulase negative staphylococci. J Clin Pathol 42:872-874 (Pubitemid 19190761)
21. Galan JE, Wolf-Watz H (2006) Protein delivery into eukaryotic cells by type III secretion machines. Nature 444:567-573 (Pubitemid 44864429)
22. Ghatak S, Agarwal RK, Bhilegaonkar KN (2006) Comparative study of cytotoxicity of Aeromonas spp. on four different cell lines. Comp Immunol Microbiol Infect Dis 29:233-241 (Pubitemid 44340727)
23. Ghosh P (2004) Process of protein transport by the type III secretion system. Microbiol Mol Biol Rev 68:771-795 (Pubitemid 39628536)
24. Greenman SB, Rutten MJ, Fowler WM, Scheffler L and others (1997) Herbicide/pesticide effects on intestinal epithelial growth. Environ Res 75:85-93 (Pubitemid 27446798)
25. Harikrishnan R, Nisha Rani M, Balasundaram C (2003) Hematological and biochemical parameters in common carp, Cyprinus carpio, following herbal treatment for Aeromonas hydrophila infection. Aquaculture 221:41-50 (Pubitemid 36409058)
26. Hazen TC, Fliermans CB, Hirsch RP, Esch GW (1978) Prevalence and distribution of Aeromonas hydrophila in the United States. Appl Environ Microbiol 36:731-738 (Pubitemid 9048650)
27. Henriques IS, Fonseca F, Alives A (2006) Occurrence and diversity of integrons and beta-lactamase genes among ampicillin resistant isolates from estuarine waters. Res Microbiol 157:938-947 (Pubitemid 44828467)
28. Heuzenroeder MW, Wong CYF, Flower RLP (1999) Distribution of two hemolytic toxin genes in clinical and environmental isolates of Aeromonas spp. Correlation with virulence in a suckling mouse model. FEMS Microbiol Lett 174:131-136 (Pubitemid 29179443)
29. Hickman-Brenner FW, MacDonald KL, Seigerwalt AG, Fanning GR, Brenner DJ, Farmer JJ III (1987) Aeromonas veronii, a new ornithine decarboxylase positive species that may cause diarrhea. J Clin Microbiol 25:900-906 (Pubitemid 17053238)
30. Hossain M (2008) Isolation of pathogenic bacteria from the skin ulcerous symptomatic gourami (Colisa lalia) through 16S rDNA analysis. Univ J Zool Rajshahi Univ 27:21-24
31. Howard SP, Buckley JT (1985) Protein export by a gramnegative bacterium: production of aerolysin by Aeromonas hydrophila. J Bacteriol 161:1118-1124 (Pubitemid 15138542)
32. Janda JM, Kokka RP (1991) The pathogenicity of Aeromonas strains relative to genospecies and pheospecies identification. FEMS Microbiol Lett 90:29-34
33. Janda JM, Abbott SL, Khashe S, Kellogg GH, Shimada T (1996) Further studies on biochemical characteristics and serologic properties of the genus Aeromonas. J Clin Microbiol 34:1930-1933 (Pubitemid 26239226)
34. Jeffries CD, Holtman DE, Guse DG (1957) Rapid method for the determining the activity of microorganisms on nucleic acids. J Bacteriol 73:590-591
35. Jeney ZS, Jeney G (1995) Recent achievements in studies on diseases of the common carp (Cyprinus carpio L.). Aquaculture 129:397-420
36. Karunasagar I, Karunasagar I, Otta SK (2003) Disease problems affecting fish in tropical environments. J Appl Aquacult 13:231-249 (Pubitemid 36370765)
37. Kim JH, Hayward CJ, Joh SJ, Heo GJ (2002) Parasitic infections in live freshwater tropical fishes imported to Korea. Dis Aquat Org 52:169-173 (Pubitemid 36028994)
38. Kimura M(1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111-120 (Pubitemid 11166431)
39. Kingombe CIB, Huys G, Tonolla M, Albert MJ, Swings J, Peduzzi R (1999) PCR detection, characterization and distribution of virulence genes in Aeromonas spp. Appl Environ Microbiol 65:5293-5302 (Pubitemid 129520612)
40. Kozinska A, Figueras MJ, Chacon MR, Soler L (2002) Phenotypic characteristics and pathogenicity of Aeromonas genomospecies isolated from common carp (Cyprinus carpio L.). J Appl Microbiol 93:1034-1041 (Pubitemid 35408579)
41. Krumperman PH (1985) Multiple antibiotic indexing of E. coli to identify high-risk sources of fecal contamination of foods. Appl Environ Microbiol 46:165-170 (Pubitemid 13045855)
42. Lallier R, Daigneault P (1984) Antigenic differentiation of pili from non-virulent fish pathogenic strains of Aeromonas hydrophila. J Fish Dis 7:509-572\
43. Lee KK, Yii KC (1996) A comparison of three methods for assaying hydrophobicity of pathogenic vibrios. Lett Appl Microbiol 23:343-346
44. Lee YK, Kim HW, Liu CL, Lee HK (2003) A simple method for DNA extraction from marine bacteria that produce extracellular materials. J Microbiol Methods 52:245-250 (Pubitemid 35379859)
45. Lehane L, Rawlin GT (2000) Topically acquired bacterial zoonoses from fish: a review. Med J Aust 173:256-259
46. Leung KY, Stevenson RMW (1988) Characteristics and distribution of extracellular proteases from Aeromonas hydrophila. J Gen Microbiol 134:151-160 (Pubitemid 18031728)
47. Lilenbaum W, Nunes ELC, Azeredo MAI (1998) Prevalence and antimicrobial susceptibility of staphylococci isolated from the skin surface of clinically normal cats. Lett Appl Microbiol 27:224-228 (Pubitemid 28499563)
48. Lilley JH, Hart D, Richards RH, Roberts RJ, Cerenius L, Soderhall K (1997) Pan-Asian spread of single fungal clone results in large-scale fish kills. Vet Rec 140:653-654 (Pubitemid 127491283)
49. Ma Z, Yang H, Li T, Luo L, Gao J (2009) Isolation and identification of pathogenic Aeromonas veronii isolated from infected Siberian sturgeon (Acipenser baerii). Wei Sheng Wu Xue Bao 49:1289-1294
50. Martínez-Murcia AJ, Saavedra MJ, Mota VR, Maier T, Stackebrandt E, Cousin S (2008) Aeromonas aquariorum sp. nov., isolated from the aquaria of ornamental fish. Int J Syst Evol Microbiol 58:1169-1175
51. Masignani V, Pizza M, Rappuoli R (2006) Molecular, functional, and evolutionary aspects of ADP ribosylating toxins. In: Alouf JE, Popoff MR (eds) The comprehensive sourcebook of bacterial protein toxins. Academic Press Elsevier, Amsterdam, p 213-244
52. McCormick JI, Hughes MS, McLoughlin MF (1995) Identification of Mycobacterium chelonae in a cichlid oscar, Astronotus ocellatus Cuvier, by direct cycle sequencing of polymerase chain reaction amplified 16s rRNA gene sequences. J Fish Dis 18:459-461
53. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival, application to proliferation and cytotoxicity assays. J Immunol Methods 65:55-63 (Pubitemid 14203433)
54. Nerland AH (1996) The nucleotide sequence of the gene encoding GCAT from Aeromonas salmonicida ssp. salmonicida. J Fish Dis 19:145-150 (Pubitemid 126565544)
55. Ormen O, Ostensvik O (2001) The occurrence of aerolysinpositive Aeromonas spp. and their cytotoxicity in Norwegian water sources. J Appl Microbiol 90:797-802 (Pubitemid 32391786)
56. Paiva PM, Nepomuceno FH (1989) On the reproduction in captivity of the oscar, Astronotus ocellatus (Cuvier), according to the mating methods (Pisces-Cichlidae). Amazoniana 10:361-377
57. Pemberton JM, Kidd SP, Schmidt R (1997) Secreted enzymes of Aeromonas. FEMS Microbiol Lett 152:1-10 (Pubitemid 27293260)
58. Plumb JA, Hanson LH (2010) Health maintenance and principal microbial diseases of cultured fishes, 3rd edn. John Wiley and Sons, Ames, IA
59. Rahman M, Navarro CP, Kuhn I, Huys G, Swings J, Mollby R (2002) Identification and characterization of pathogenic Aeromonas veronii biovar sobria associated with epizootic ulcerative syndrome in fish in Bangladesh. Pak J Biol Sci 68:650-655 (Pubitemid 34121042)
60. Rahman MM, Somsiri T, Tajima K, Ezura Y (2004) Distribution of Aeromonas sp. emphasizing on a newly identified species of Aeromonas sp. T8 isolated from fish and aquatic animals in Southeast Asia. Pak J Biol Sci 7:258-268
61. Reddy GSM, Aggarwal RK, Matsumotto GI, Sivaji SI (2000) Arthrobacter flavus sp. nov., a psychrophilic bacterium isolated from a pond in McMurdo Dry Valley, Antarctica. Int J Syst Evol Microbiol 50:1553-1561 (Pubitemid 30601968)
62. Reed MJ, Muench M (1938) A simple method for estimating fifty percent endpoints. Am J Hyg 27:493-497
63. Rosenberg K, Gutnick D, Rosenberg E (1980) Adherence of bacteria to hydrocarbons, a simple method for measuring cell-surface hydrophobicity. FEMS Microbiol Lett 9:29-33 (Pubitemid 11245858)
64. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406-425
65. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
66. Santos JA, Gonzalez CJ, Otero A, Garcia-Lopez ML (1999) Hemolytic and siderophore production in different Aeromonas species isolated from fish. Appl Environ Microbiol 65:5612-5614 (Pubitemid 129520661)
67. Scoaris DDO, Colacite J, Nakamura CV, Nakamura TU, Filho BAA, Filho BPD (2008) Virulence and antibiotic susceptibility of Aeromonas spp. isolated from drinking water. Antonie Leeuwenhoek 93:111-122
68. Sha J, Pillai L, Fald AA, Galindo CL, Erova TE, Chopra AK (2005) The type III secretion system and cytotoxic enterotoxin alter the virulence of Aeromonas hydrophila. Infect Immun 73:6446-6457 (Pubitemid 41368806)
69. Shao J, Liu J, Xiang L (2004) Aeromonas hydrophila induces apoptosis in Carassius auratus lymphocytes in vitro. Aquaculture 229:11-23 (Pubitemid 38051225)
70. Shome R, Shome BR, Mazumder Y, Das A, Kumar A, Rahman H, Bujarbaruah KM (2005) Abdominal dropsy disease in major carps of Meghalaya: isolation and characterization of Aeromonas hydrophila. Curr Sci 88:1897-1900 (Pubitemid 41602957)
71. Silver AC, Graf J (2009) Prevalence of genes encoding the type three secretion system and the effectors AexT and AexU in the Aeromonas veronii group. DNA Cell Biol 28:383-388
72. Soltani M, Mirzargar SS, Abrahimzadeh HA (1998) Occurrence of a motile Aeromonas septicemia in the imported ornamental fish, oscar, Astronotus ocellatus: isolation, characterization, and pathogenicity. J Fac Vet Med Univ Tehran 53:63-65
73. Sreedharan K (2008) Aeromonas associated with freshwater ornamental fish culture systems: characterization, pathogenicity and management. PhD thesis, Cochin University of Science and Technology
74. Staeck W, Linke H (1995) American cichlids. II. Large cichlids: a handbook for their identification, care, and breeding. Tetra Press, Melle
75. Stuber K, Frey J, Burnens AP, Kuhnert P (2003) Detection of type III secretion genes as a general indicator of bacterial virulence. Mol Cell Probes 17:25-32 (Pubitemid 36287307)
76. Sudheesh PS, Jie K, Xu H (2002) Random amplified polymorphic DNA-PCR typing of Vibrio parahaemolyticus and V. alginolyticus isolated from cultured shrimps. Aquaculture 207:11-17 (Pubitemid 34489830)
77. Swift S, Lynch JM, Fish L, Kirke DF, Tomas JM, Stewart GSAB, Williams P (1999) Quarum sensing-dependent regulation and blockade of exoprotease production in Aeromonas hydrophila. Infect Immun 67:5192-5199 (Pubitemid 29455291)
78. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596-1599 (Pubitemid 47236692)
79. Tenover FC, Lancaster MV, Hill BC (1988) Characterization of staphylococci with reduced susceptibilities to vancomycin and other glycopeptides. J Clin Microbiol 36: 1020-1027
80. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680 (Pubitemid 24354800)
81. Timpe JM, Holm MM, Vanlenberg SL, Basrur V, Lafontaine ER (2003) Identification of a Moraxella catarrhalis outer membrane protein exhibiting both adhesion and lipolytic activities. Infect Immun 71:4341-4350 (Pubitemid 36919898)
82. Toksen E (2006) Argulus foliacesus (Crustacea: Branchiura) infestation on oscar, Astronotus ocellatus (Cuvier, 1829) and its treatment. EU J Fish Aquat Sci 23:177-179
83. Trakhna F, Harf-Monteil C, Abdelnour A, Maaroufi A, Gadonna-Widehem P (2009) Rapid Aeromonas hydrophila identification by TaqMan PCR assay: comparison with a phenotypic method. Lett Appl Microbiol 49:186-190
84. Tukmechi A, Hobbenaghi R, Holasoo RH, Morvaridi A (2009) Streptococcosis in a pet fish, Astronotus ocellatus: a case study. World Acad Sci Eng Tech 49:14-15
85. van der Marel M, Schroers V, Neuhaus H, Steinhagen D (2008) Chemotaxis towards, adhesion to, and growth in carp gut mucus of two Aeromonas hydrophila strains with different pathogenicity for common carp, Cyprinus carpio L. J Fish Dis 31:321-330 (Pubitemid 351502532)
86. Vilches S, Wilhelms M, Yu HB, Leung KY, Thomas JM (2008) Aeromonas hydrophila AH-3 AexT is an ADP-ribosylating toxin secreted through the type III secretion system. Microb Pathog 44:1-12 (Pubitemid 350236687)
87. Von B (2010) Fundamental information regarding oscar fish. http://ezinearticles.com/?Fundamental-Information- Regarding-Oscar-Fish&id= 4572555
88. Wahli T, Burr SE, Pugovkin D, Mueller O, Frey J (2005) Aeromonas sobria, a causative agent of disease in farmed perch, Perca fluviatilis L. J Fish Dis 28:141-150 (Pubitemid 40441070)
89. Wilcox MH, Cook AM, Eley A, Spencer RC (1992) Aeromonas spp. as a potential cause of diarrhoea in children. J Clin Pathol 45:959-963
90. Wu CJ, Wu JJ, Yan JJ, Lee HC and others (2007) Clinical significance and distribution of putative virulence markers of 116 consecutive clinical Aeromonas isolates in southern Taiwan. J Infect 54:151-158