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Organic and Biomolecular Chemistry
Volumn 9, Issue 11, 2011, Pages 4021-4024
Improvement of catalytic properties of Candida Rugosa lipase by sol-gel encapsulation in the presence of magnetic calix[4]arene nanoparticles
Author keywords

[No Author keywords available]
Indexed keywords

CALIX[4]ARENES; CANDIDA RUGOSA LIPASE; CATALYTIC PROPERTIES; FREE ENZYME; MAGNETIC NANOPARTICLES; N-METHYLGLUCAMINE; S-NAPROXEN; SOL-GEL ENCAPSULATION; TETRAETHOXYSILANES;
EID: 79956271394     PISSN: 14770520     EISSN: None     Source Type: Journal    
DOI: 10.1039/c1ob05115f     Document Type: Article
Times cited : (60)
References (25)
  • 13
    • 79956279249 scopus 로고    scopus 로고
    • R. M. Kazlauskas, U. T. Bornscheur, H. J. Rehm and G. Reed, vol. 8, Wiley V. H. C. Verlag, Gmbh Germany, Berlin, 268-300
    • Biotechnology-A Comprehensive Treatise, ed., R. M. Kazlauskas,,, U. T. Bornscheur,,, H. J. Rehm, and, G. Reed,, vol. 8, Wiley V. H. C. Verlag, Gmbh Germany, Berlin, 1998, pp. 268-300
    • (1998) Biotechnology-A Comprehensive Treatise, Ed.
  • 19
    • 0041687879 scopus 로고    scopus 로고
    • For the lipase immobilization onto N-methylglucamine based calix[4]arene magnetic nanoparticles, 60 mg of Candida rugosa lipase was reacted with 50 mg of N-methylglucamine based calix[4]arene magnetic nanoparticles in 0.4 mL of phosphate buffer (0.05 M; pH 7.0) under vigorous stirring on a horizontal shaker at room temperature. Then 0.1 mL of aqueous PVA (4% w/v), aqueous sodium fluoride (50 μL of a 1 M solution) and isopropyl alcohol (0.1 mL) were added, and the mixture was homogenized using a shaker. Then the OTES (2.5 mmol) and TEOS (0.5 mmol; 120 μL) were added and the mixture was agitated once more for 10-15 s. Gelation was usually observed within seconds or minutes while gently shaking the reaction vessel. The gel was lyophilizated and successively washed with distilled water (10 mL) and isopropyl alcohol (10 mL). The resulting encapsulated lipases were kept at 4°C prior to use
    • M. T. Reetz P. Tielmann W. Wisenhöfer W. Könen A. Zonta Adv. Synth. Catal. 2003 345 717 728
    • (2003) Adv. Synth. Catal. , vol.345 , pp. 717-728
    • Reetz, M.T.1    Tielmann, P.2    Wisenhöfer, W.3    Könen, W.4    Zonta, A.5
  • 22
    • 0035085171 scopus 로고    scopus 로고
    • -1; the UV detector was fixed at 254 nm) The hydrolysis reactions were maintained in an aqueous phase-organic solvent batch reaction system that consisted of 2 mL isooctane as solvent dissolving racemic Naproxen methyl ester (20 mM) and 2 mL buffer solution (pH 7.0, 50 mM phosphate buffer solution) including encapsulated lipase (Calix-MN-SE-Lipase) (5-50 mg depending on the activity). The reactions were carried out on a horizontal shaker at 200 rpm at 30°C and samples drawn from isooctane phase at 24 h were analyzed by HPLC to calculate the conversion and enantioselectivity
    • S. Johri V. Verma R. Parshad S. Koul S. C. Taneja G. N. Qazi Bioorg. Med. Chem. 2001 9 269 273
    • (2001) Bioorg. Med. Chem. , vol.9 , pp. 269-273
    • Johri, S.1    Verma, V.2    Parshad, R.3    Koul, S.4    Taneja, S.C.5    Qazi, G.N.6

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.