Mechanistic evaluation of MelA α-galactosidase from citrobacter freundii: A family 4 glycosyl hydrolase in which oxidation is rate-limiting

Biochemistry

Volumn 50, Issue 20, 2011, Pages 4298-4308

  Chakladar, Saswati ^a   Cheng, Lydia ^a   Choi, Mary ^a   Liu, James ^a   Bennet, Andrew J ^a  

^a SIMON FRASER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATED SUBSTRATES; COFACTORS; DEUTERIUM KINETIC ISOTOPE EFFECT; ELIMINATION-ADDITION MECHANISM; GALACTOSIDASES; GLYCOSYL HYDROLASES; HYDROXYL GROUPS; ISOTOPIC SUBSTITUTION; LEAVING GROUPS; MECHANISM OF ACTION; MERCAPTOETHANOL; OPTIMAL ACTIVITY; RATE LIMITING; RATE-LIMITING STEPS; REACTION MECHANISM; RECOMBINANT ENZYMES; SINGLE-STEP; VIRTUAL TRANSITIONS;

CATALYSIS; DEUTERIUM; ENZYME ACTIVITY; ESCHERICHIA COLI; GENES; HYDROLASES; HYDROLYSIS; MANGANESE; MANGANESE COMPOUNDS; OXIDATION; PHENOLS; REACTION INTERMEDIATES; THREE DIMENSIONAL;

SUBSTRATES;

ALPHA GALACTOSIDASE; GLYCOSYL HYDROLASE FAMILY 4; MELA GALACTOSIDASE; PHENOL; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; CARBON NUCLEAR MAGNETIC RESONANCE; CHEMICAL BOND; CHEMICAL REACTION; CHEMICAL STRUCTURE; CITROBACTER FREUNDII; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; HYDROLYSIS; KINETIC ISOTOPE EFFECT; KINETICS; MOLECULAR CLONING; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTON NUCLEAR MAGNETIC RESONANCE;

ALPHA-GALACTOSIDASE; CARBOHYDRATE SEQUENCE; CITROBACTER FREUNDII; DEUTERIUM; GALACTOSE; GLYCOLIPIDS; GLYCOPROTEINS; HYDROLYSIS; KINETICS; MELIBIOSE; MOLECULAR WEIGHT; OXIDATION-REDUCTION; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, QUATERNARY; SYMPORTERS;

CITROBACTER FREUNDII; ESCHERICHIA COLI;

EID: 79956215215     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi101808h     Document Type: Article

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