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Volumn 21, Issue 11, 2011, Pages 3483-3487

Anti-inflammatory activity of constituents from Glechoma hederacea var. longituba

Author keywords

COX 2; Glechoma hederacea; Inflammation; iNOS; Nf B; Rosmarinic acid

Indexed keywords

3' O METHYLROSMARINIC ACID; ANTIINFLAMMATORY AGENT; BENZYL 4' HYDROXYBENZOYL 3' O BETA DEXTROGLUCOPYRANOSIDE; CYCLOOXYGENASE 2 INHIBITOR; ETHYLROSMARINIC ACID; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INDUCIBLE NITRIC OXIDE SYNTHASE; METHYLISOFERULOYL 7 (3,4 DIHYDROXYPHENYL)LACTATE; METHYLROSMARINIC ACID; PLANT EXTRACT; PYRROLIDINE DITHIOCARBAMATE; ROSMARINIC ACID; UNCLASSIFIED DRUG;

EID: 79956123316     PISSN: 0960894X     EISSN: 14643405     Source Type: Journal    
DOI: 10.1016/j.bmcl.2011.02.002     Document Type: Article
Times cited : (50)

References (20)
  • 14
    • 79956125000 scopus 로고    scopus 로고
    • note
    • 3OD) in Table 1.
  • 16
    • 79956126462 scopus 로고    scopus 로고
    • note
    • 5 cells/mL (1.5 mL) in 6-well and grown for 24 h. All cells were transfected by the lipofectamine LTX (Invitrogen, Carlsbad, CA) as described by the manufacturer. Luciferase activity was assayed using an LB 953 Autolumat (EG&G Berthold, Nashua, NH) and was normalized based on the expression of RSV-β-galactosidase. β-Galactosidase activity was assayed colorimetrically. NF-κB-Luc plasmid was provided by Dr. Kyoon E. Kim (Department of Biochemistry, Chungnam National University).
  • 17
    • 79956136431 scopus 로고    scopus 로고
    • note
    • 490 nm of the dissolved formazan product after addition of MTS for 30 min as described by the manufacturer (Promega, Madison, WI).
  • 18
    • 79956115699 scopus 로고    scopus 로고
    • note
    • Total RNA extraction was isolated with Easy-blue reagent (Intron biotechnology, Seoul, Korea). Approximately 2 μg of total RNA were reverse transcribed using moloney murine leukemia virus (MMLV) reverse transcriptase and oligo-dT primers (Promega, Madison, WI) for 1 h at 42 °C. PCR for synthetic cDNA was performed using Taq polymerase pre-mixture (TaKaRa, Japan). PCR products were subjected to electrophoresis on 1% agarose gels and stained with EtBr. PCR was conducted with the following primer pairs: iNOS sense 5′-TCATCCGCTATGCTGGCTAC-3′, iNOS antisense 5′- CTCAGGGTCACGGCCATTG-3′, COX-2 sense 5′-GCCCAGCACTTCACGCATCAG- 3′, COX-2 antisense 5′-GACCAGGCACCAGACCAAAGACC-3′, GAPDH sense 5′-TGTTGCCATCAATGACCCCTT-3′, and GAPDH antisense 5′-CTCCACGACGTACTCAGCG-3′. The specificity of products generated by each set of primers was examined using gel electrophoresis.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.