High-throughput profiling of mature MicroRNA by real-time PCR

Neuromethods

Volumn 58, Issue , 2011, Pages 113-121

  Jiang, Jinmai ^a   Lee, Eun Joo ^a   Piper, Melissa G ^a   Marsh, Clay B ^a   Schmittgen, Thomas D ^a  

^a NONE

Author keywords

Gene expression; MicroRNA; Noncoding RNA

Indexed keywords

MICRORNA;

ANALYTICAL ERROR; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RNA ISOLATION; ROBOTICS;

EID: 79956111745     PISSN: 08932336     EISSN: 19406045     Source Type: Book Series    
DOI: 10.1007/978-1-61779-114-7_7     Document Type: Article

References (12)

1. Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004;116: 281-97.
2. Barbarotto E, Schmittgen TD, Calin GA. MicroRNAs and cancer: profle, profle, pro-fle. Int J Cancer 2008;122:969-77.
3. Calin GA, Croce CM. MicroRNA signatures in human cancers. Nat Rev Cancer 2006;6: 857-66. (Pubitemid 44629897)
4. Liu CG, Calin GA, Meloon B, et al. An oligonu-cleotide microchip for genome-wide microRNA profling in human and mouse tissues. Proc Natl Acad Sci USA 2004;101:9740-4. (Pubitemid 38869306)
5. Wang H, Ach RA, Curry B. Direct and sensitive miRNA profling from low-input total RNA. RNA 2007;13:151-9. (Pubitemid 46026189)
6. Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc 2008;3:1101-8. (Pubitemid 351818697)
7. Griffths-Jones S. The microRNA Registry. Nucleic Acids Res 2004;32:D109-11.
8. Mestdagh P, Feys T, Bernard N, et al. High-throughput stem-loop RT-qPCR miRNA expression profling using minute amounts of input RNA. Nucleic Acids Res 2008; 36:e143.
9. Ratajczak J, Wysoczynski M, Hayek F, Janowska-Wieczorek A, Ratajczak MZ. Membrane-derived microvesicles: important and underappreciated mediators of cell-to-cell communication. Leukemia 2006;20: 1487-95. (Pubitemid 44264090)
10. Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol 2007;9:654-9. (Pubitemid 47214727)
11. Hunter MP, Ismail N, Zhang X, et al. Detection of microRNA expression in human peripheral blood microvesicles. PLoS ONE 2008;3:e3694.
12. Vandesompele J, De Preter K, Pattyn F, et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002;3:RESEARCH0034.