Development of an automated MODS plate reader to detect early growth of Mycobacterium tuberculosis

Journal of Microscopy

Volumn 242, Issue 3, 2011, Pages 325-330

  Comina, G ^a   Mendoza, D ^a   Velazco, A ^a   Coronel, J ^b   Sheen, P ^b   Gilman, R H ^b,c   Moore, D A J ^b,c,d,e   Zimic, Mirko ^b  

^a UNIVERSIDAD NACIONAL DE INGENIERÍA   (Peru)

^b UNIVERSIDAD PERUANA CAYETANO HEREDIA   (Peru)

^c JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

^d IMPERIAL COLLEGE LONDON   (United Kingdom)

^e LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

Author keywords

Autofocussing algorithm; Automated MODS; MODS; MODS plate reader

Indexed keywords

IMAGE ACQUISITION; OPEN SOURCE SOFTWARE; OPEN SYSTEMS; PATTERN RECOGNITION; PERSONAL COMPUTERS; TUBES (COMPONENTS);

AUTOFOCUSSING ALGORITHM; AUTOMATED MICROSCOPIC OBSERVATION DRUG SUSCEPTIBILITY; DRUG SUSCEPTIBILITY; MICROSCOPIC OBSERVATION DRUG SUSCEPTIBILITY; MICROSCOPIC OBSERVATION DRUG SUSCEPTIBILITY PLATE READER; MICROSCOPIC OBSERVATIONS;

AUTOMATION;

ALGORITHM; ARTICLE; AUTOMATION; BACTERIAL GROWTH; BACTERIUM CULTURE; ELECTRONICS; IMAGE ANALYSIS; LABORATORY PERSONNEL; MICROSCOPIC OBSERVATION DRUG SUSCEPTIBILITY PLATE READER; MICROSCOPY; MULTIDRUG RESISTANT TUBERCULOSIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; TUBERCULOSIS;

MYCOBACTERIUM TUBERCULOSIS;

EID: 79955997132     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2010.03477.x     Document Type: Article

References (21)

1. Arias, M., Mello, F.C., Pavon, A., et al. (2007) Clinical evaluation of the microscopic-observation drug-susceptibility assay for detection of tuberculosis. Clin. Infect. Dis. 44, 674-680.
2. Bae, W.H., Salas, A., Brady, M.F., et al. (2008) Reducing the string test intra-gastric downtime for detection ofMycobacterium tuberculosis. Int. J. Tuberc. Lung Dis. 12, 1436-1440.
3. Brady, M.F., Coronel, J., Gilman, R.H. & Moore, D.A. (2008) The MODS method for diagnosis of tuberculosis and multidrug resistant tuberculosis. J. Vis. Exp. 11, 845.
4. Caviedes, L., Lee, T.S., Gilman, R.H., Sheen, P., Spellman, E., Lee, E.H., Berg, D.E. & Montenegro-James, S. (2000) Rapid, efficient detection and drug susceptibility testing ofMycobacterium tuberculosisin sputum by microscopic observation of broth cultures. The Tuberculosis Working Group in Peru. J. Clin. Microbiol. 38, 1203-1208.
5. Chihota, V.N., Grant, A.D., Fielding, K., Ndibongo, B., van Zyl, A., Muirhead, D. & Churchyard, G.J. (2010) Liquid vs. solid culture for tuberculosis: performance and cost in a resource-constrained setting. Int. J. Tuberc. Lung Dis. 14, 1024-1031.
6. Fitzwater, S.P., Caviedes, L., Gilman, R.H., et al. (2010) Prolonged infectiousness of tuberculosis patients in a directly observed therapy short-course program with standardized therapy. Clin. Infect. Dis, 51, 371-378.
7. Minion, J., Leung, E., Menzies, D. & Pai, M. (2010) Microscopic-observation drug susceptibility and thin layer agar assays for the detection of drug resistant tuberculosis: a systematic review and meta-analysis. Lancet Infect. Dis. 10, 688-698.
8. Moore, D.A., Caviedes, L., Gilman, R.H., et al. (2006a) Infrequent MODS TB culture cross-contamination in a high-burden resource-poor setting. Diagn. Microbiol. Infect. Dis. 56, 35-43.
9. Moore, D.A., Evans, C.A., Gilman, R.H., et al. (2006b) Microscopic-observation drug-susceptibility assay for the diagnosis of TB. N. Engl. J. Med. 355, 1539-1550.
10. Moore, D.A., Mendoza, D., Gilman, R.H., et al. (2004) Microscopic observation drug susceptibility assay, a rapid, reliable diagnostic test for multidrug-resistant tuberculosis suitable for use in resource-poor settings. J. Clin. Microbiol. 42, 4432-4437.
11. Oberhelman, R.A., Soto-Castellares, G., Caviedes, L., Castillo, M.E., Kissinger, P., Moore, D.A., Evans, C. & Gilman, R.H. (2006) Improved recovery ofMycobacterium tuberculosisfrom children using the microscopic observation drug susceptibility method. Pediatrics 118, e100-e106.
12. Oberhelman, R.A., Soto-Castellares, G., Gilman, R.H., et al. (2010) Diagnostic approaches for paediatric tuberculosis by use of different specimen types, culture methods, and PCR: a prospective case-control study. Lancet Infect. Dis. 10, 612-620.
13. Osibote, O.A., Dendere, R., Krishnan, S. & Douglas, T.S. (2010) Automated focusing in bright-field microscopy for tuberculosis detection. J. Microsc. 240, 155-163.
14. Park, W.G., Bishai, W.R., Chaisson, R.E. & Dorman, S.E. (2002) Performance of the microscopic observation drug susceptibility assay in drug susceptibility testing forMycobacterium tuberculosis. J. Clin. Microbiol. 40, 4750-4752.
15. Reddy, K.P., Brady, M.F., Gilman, R.H., et al. (2010) Microscopic observation drug susceptibility assay for tuberculosis screening before isoniazid preventive therapy in HIV-infected persons. Clin. Infect. Dis. 50, 988-996.
16. Shiferaw, G., Woldeamanuel, Y., Gebeyehu, M., Girmachew, F., Demessie, D. & Lemma, E. (2007) Evaluation of microscopic observation drug susceptibility assay for detection of multidrug-resistantmycobacterium tuberculosis. J. Clin. Microbiol. 45, 1093-1097.
17. Tovar, M., Siedner, M.J., Gilman, R.H., et al. (2008) Improved diagnosis of pleural tuberculosis using the microscopic-observation drug-susceptibility technique. Clin. Infect. Dis. 46, 909-912.
18. WHO (2009) WHO strategic and technical advisory group for tuberculosis report on conclusions and recommendations of ninth meeting. 9-11 November 2009. WHO, Geneva. Available at.
19. Yazdanfar, S., Kenny, K.B., Tasimi, K., Corwin, A.D., Dixon, E.L. & Filkins, R.J. (2008) Simple and robust image-based autofocusing for digital microscopy. Opt. Express 16, 8670-8677.
20. Zimic, M., Coronel, J., Gilman, R.H., Luna, C.G., Curioso, W.H. & Moore, D.A. (2009) Can the power of mobile phones be used to improve tuberculosis diagnosis in developing countries Trans. R. Soc. Trop. Med. Hyg. 103, 638-640.
21. Zimic, M., Velazco, A., Comina, G., et al. (2010) Development of low-cost inverted microscope to detect early growth ofMycobacterium tuberculosisin MODS culture. PLoS One 5, e9577.