Automated focusing in bright-field microscopy for tuberculosis detection

Journal of Microscopy

Volumn 240, Issue 2, 2010, Pages 155-163

  Osibote, O A ^a   Dendere, R ^a   Krishnan, S ^a   Douglas, T S ^a  

^a UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

Autofocusing; Automated microscopy; Focus measure; Tuberculosis; Ziehl Neelsen

Indexed keywords

AUTOMATION; FOCUSING;

AUTO-FOCUSING; AUTOMATED MICROSCOPY; BRIGHT-FIELD MICROSCOPY; CORE COMPONENTS; CURVES FITTINGS; FOCUS ALGORITHMS; FOCUS MEASURE; MYCOBACTERIUM TUBERCULOSIS; TUBERCULOSIS; ZIEHL-NEELSEN;

CURVE FITTING;

ACCURACY; ALGORITHM; ARTICLE; AUTOMATION; BRIGHT FIELD MICROSCOPY; MYCOBACTERIUM TUBERCULOSIS; PRIORITY JOURNAL; SPUTUM SMEAR; TUBERCULOSIS;

MYCOBACTERIUM TUBERCULOSIS;

EID: 77958190259     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2010.03389.x     Document Type: Article

References (30)

1. Brenner, J.F., Dew, B.S., Horton, J.B., King, T., Neurath, P.W. & , Selles, W.D. (1976) Automated microscope for cytologic research - preliminary evaluation. , J. Histochem. Cytochem. 24, 100-111.
2. Firestone, L., Cook, K., Culp, K., Talsania, N. & , Preston, K. (1991) Comparison of autofocus methods for automated microscopy. , Cytometry , 12, 195-206.
3. Forero, M.G., Sroubek, F. & , Cristobal, G. (2004) Identification of tuberculosis bacteria based on shape and color. , Real-Time Imaging. 10, 251-262.
4. Forero, M.G., Cristobal, G. & , Desco, M. (2006) Automatic identification of Mycobacterium tuberculosis by Gaussian mixture models. , J. Microsc.-Oxford , 223, 120-132.
5. Geusebroek, J., Cornelissen, F., Smeulders, A.W.M. & , Geerts, G. (2000) Robust autofocusing in microscopy. , Cytometry , 39, 1-9.
6. Gonzalez, R.C. & , Woods R.E. (2001) Digital Image Processing. Prentice Hall, New Jersey,.
7. Groen, F.C.A., Young, I.T. & , Ligthart, G. (1985) A comparison of different focus functions for use in autofocus algorithms. , Cytometry , 6, 81-91.
8. He, J., Zhou, R.Z. & , Hong, Z.L. (2003) Modified fast climbing search auto-focus algorithm with adaptive step size searching technique for digital camera. , IEEE Transact. Consum. Electron. 49, 257-262.
9. Huang, W. & , Jing, Z. (2007) Evaluation of focus measures in multi-focus image fusion. , Pattern Recogn. Lett. 28, 493-500.
10. Khutlang, R., Krishnan, S., Whitelaw, A. & , Douglas, T.S. (2010) Automated detection of tuberculosis in Ziehl-Neelsen stained sputum smears using two one-class classifiers. , J. Microsc.-Oxford. 237, 96-102.
11. Khutlang, R., Krishnan, S., Dendere, R., Whitelaw, A., Veropoulos, K., Learmonth, G. & , Douglas, T.S. (in press) Classification of Mycobacterium tuberculosis in images of Z.N.-stained sputum smears. , IEEE Transact. Informat. Technol. Biomed., DOI, .
12. Krotkov, E. (1987) Focusing. , Int. J. Comput. Vis. 1, 223-237.
13. Liu, X.Y., Wang, W.H. & , Sun, Y. (2007) Dynamic evaluation of autofocusing for automated microscopic analysis of blood smear and pap smear. , J. Microsc.-Oxford , 227, 15-23.
14. Malik, A.S. & , Choi, T.S. (2008) A novel algorithm for estimation of depth map using image focus for 3D shape recovery in the presence of noise. , Pattern Recogn. 41, 2200-2225.
15. Nayar, S.K. & , Nakagawa, Y. (1994) Shape from Focus. , IEEE Transact. Pattern Anal. Mach. Intell. 16, 824-831.
16. Nikon MicroscopyU: Articles - Formulas, last accessed 14 December 2009.
17. Pech-Pacheco, J.L., Cristobal, G., Chamorro-Martinez, J. & , Fernandez-Valdivia, J. (2000) Diatom autofocusing in brighfield microscopy: a comparative study. , Proceedings of the 15th International Conference on Pattern Recognition, Barcelona, p. 3.
18. Sadaphal, P., Rao, J., Comstock, G.W. & , Beg, M.F. (2008) Image processing techniques for identifying Mycobacterium tuberculosis in Ziehl-Neelsen stains. , Int. J. Tuberc. Lung Dis. 12, 579-582.
19. Santos, A., De Solorzano, C.O., Vaquero, J.J., Pena, J.M., Malpica, N. & , Del Pozo, F. (1997) Evaluation of autofocus functions in molecular cytogenetic analysis. , J. Microsc.-Oxford , 188, 264-272.
20. Smith, J.O. (2008) Spectral Audio Signal Processing, online book, last accessed 5 July 2009.
21. Steingart, K., Henry, M., Ng, V., et al., (2006) Fluorescence versus conventional sputum smear microscopy for tuberculosis: a systematic review. , Lancet Infect. Dis. 6, 570-581.
22. Subbarao, M. & , Tyan, J.K. (1998) Selecting the optimal focus measure for autofocusing and depth-from-focus. , IEEE Transact. Pattern Anal. Mach. Intell. 20, 864-870.
23. Sun, Y., Duthaler, S. & , Nelson, B.J. (2004) Autofocusing in computer microscopy: selecting the optimal focus algorithm. , Microsc. Res. Tech. 65, 139-149.
24. Svahn, F. (1996) Tools and methods to obtain a passive autofocus system. MSc Thesis, Tekniska Hogskolan Linkoping.
25. Valdecasas, A.G., Marshall, D., Becerra, J.M. & , Terrero, J.J. (2001) On the extended depth of focus algorithms for bright field microscopy. , Micron , 32, 559-569.
26. Veropoulos, K., Learmonth, G., Campbell, C., Knight, B. & , Simpson, J. (1999) Automated identification of tubercle bacilli in sputum - A preliminary investigation. , Anal. Quant. Cytol. Histol. 21, 277-282.
27. Vollath, D. (1988) The influence of the scene parameters and of noise on the behavior of automatic focusing algorithms. , J. Micros.-Oxford , 151, 133-146.
28. Xie, H, Rong, W.B. & , Sun, L.N. (2007) Construction and evaluation of a wavelet-based focus measure for Microscopy Imaging. , Microsc. Res. Tech. 70, 987-995.
29. Yazdanfar, S., Kenny, K.B., Tasimi, K., Corwin, A.D., Dixon, E.L. & , Filkins, R.J. (2008) Simple and robust image-based autofocusing for digital microscopy. , Opt. Express. 16, 8670-8677.
30. Yeo, T.T.E., Ong, S.H., Jayasooriah -, Sinniah, R. (1993) Autofocusing for tissue microscopy. , Image Vis. Comput. 11, 629-639.