Fibroblast expression of β-smooth muscle actin, β2β1 integrin and βvβ3 integrin: Influence of surface rigidity

Experimental and Molecular Pathology

Volumn 91, Issue 1, 2011, Pages 394-399

  Jones, Christine ^a   Ehrlich, H Paul ^a  

^a PENN STATE COLLEGE OF MEDICINE   (United States)

Author keywords

Smooth muscle actin.; Collagen; Integrin; Myofibroblast; Polyacrylamide gel; Tension

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; COLLAGEN TYPE 1; POLYACRYLAMIDE GEL; VERY LATE ACTIVATION ANTIGEN 2; VITRONECTIN RECEPTOR; ACTA2 PROTEIN, HUMAN; ACTIN;

ARTICLE; CELL STRUCTURE; CELL SURFACE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNOHISTOLOGY; IN VITRO STUDY; MEMBRANE FORMATION; MICROFILAMENT; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; RIGIDITY; SKIN FIBROBLAST; STRESS FIBER; SURFACE PROPERTY; SURFACE TENSION; WESTERN BLOTTING; WOUND CONTRACTION; CELL CULTURE; CELL DIFFERENTIATION; CELL SHAPE; CYTOLOGY; CYTOSKELETON; EXTRACELLULAR MATRIX; GEL; MALE; METABOLISM; MYOFIBROBLAST; PHYSIOLOGY; PREPUCE; WOUND HEALING;

ACTINS; CELL DIFFERENTIATION; CELL SHAPE; CELLS, CULTURED; CYTOSKELETON; EXTRACELLULAR MATRIX; FORESKIN; GELS; HUMANS; INTEGRIN ALPHA2BETA1; INTEGRIN ALPHAVBETA3; MALE; MYOFIBROBLASTS; SURFACE TENSION; WOUND HEALING;

EID: 79955976415     PISSN: 00144800     EISSN: 10960945     Source Type: Journal    
DOI: 10.1016/j.yexmp.2011.04.007     Document Type: Article

References (29)

1. Aarabi S., Bhatt K.A., Shi Y., et al. Mechanical load initiates hypertrophic scar formation through decreased cellular apoptosis. FASEB J. 2007, 21:3250-3261.
2. Abramoff M.D., Magelhaes P.J., Ram S.J. Imag processing with Image. J. Biophotonics Internal 2004, 11:36-42.
3. Arora P.D., Narani N., McCulloch C.A. The compliance of collagen gels regulates transforming growth factor-beta induction of alpha-smooth muscle actin in fibroblasts. Am. J. Pathol. 1999, 154:871-882.
4. Bell E., Ivarsson B., Merrill C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc. Nat. Acad. Sci. U.S.A 1979, 76:1274-1278.
5. Beningo K.A., Lo C.M., Wang Y.L. Flexible polyacrylamide substrata for the analysis of mechanical interactions at cell-substratum adhesions. Meth. Cell Biol. 2002, 69:325-339.
6. Boemi L., Allison G.M., Graham W.P., et al. Differences between scar and dermal cultured fibroblasts derived from a patient with recurrent abdominal incision wound herniation. Plast. Reconstr. Surg. 1999, 104:1397-1405.
7. Derderian C.A., Bastidas N., Lerman O.Z., et al. Mechanical strain alters gene expression in an in vitro model of hypertrophic scarring. Ann. Plast. Surg. 2005, 55:69-75. discussion 75.
8. Desmouliere A., Rubbia-Brandt L., Abdiu A., et al. Alpha-smooth muscle actin is expressed in a subpopulation of cultured and cloned fibroblasts and is modulated by gamma-interferon. Exp. Cell Res. 1992, 201:64-73.
9. Desmouliere A., Geinoz A., Gabbiani F., et al. Transforming growth factor-beta 1 induces alpha-smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts. J. Cell Biol. 1993, 122:103-111.
10. Desmouliere A., Darby I.A., Gabbiani G. Normal and pathologic soft tissue remodeling: role of the myofibroblast, with special emphasis on liver and kidney fibrosis. Lab. Invest. 2003, 83:1689-1707.
11. Desmouliere A., Chaponnier C., Gabbiani G. Tissue repair, contraction, and the myofibroblast. Wound Repair Regen. 2005, 13:7-12.
12. Eckes B., Zweers M.C., Zhang Z.G., et al. Mechanical tension and integrin alpha 2 beta 1 regulate fibroblast functions. J. Investig. Dermatol. Symp. Proc. 2006, 11:66-72.
13. Ehrlich H.P. Wound closure: evidence of cooperation between fibroblasts and collagen matrix. Eye (Lond) 1988, 2(Pt 2):149-157.
14. Ehrlich H.P., Rittenberg T. Differences in the mechanism for high-versus moderate-density fibroblast-populated collagen lattice contraction. J. Cell. Physiol. 2000, 185:432-439.
15. Ehrlich H.P., Cremona O., Gabbiani G. The expression of alpha 2 beta 1 integrin and alpha smooth muscle actin in fibroblasts grown on collagen. Cell Biochem. Funct. 1998, 16:129-137.
16. Ehrlich H.P., Allison G.M., Leggett M. The myofibroblast, cadherin, alpha smooth muscle actin and the collagen effect. Cell Biochem. Funct. 2006, 24:63-70.
17. Gullberg D., Terracio L., Borg T.K., et al. Identification of integrin-like matrix receptors with affinity for interstitial collagens. J. Biol. Chem. 1989, 264:12686-12694.
18. Hinz B., Gabbiani G. Mechanisms of force generation and transmission by myofibroblasts. Curr. Opin. Biotechnol. 2003, 14:538-546.
19. Hinz B., Mastrangelo D., Iselin C.E., et al. Mechanical tension controls granulation tissue contractile activity and myofibroblast differentiation. Am. J. Pathol. 2001, 159:1009-1020.
20. Jiang G., Huang A.H., Cai Y., et al. Rigidity sensing at the leading edge through alpha v beta 3 integrins and RPTPalpha. Biophys. J. 2006, 90:1804-1809.
21. Klein C.E., Dressel D., Steinmayer T., et al. Integrin alpha 2 beta 1 is upregulated in fibroblasts and highly aggressive melanoma cells in three-dimensional collagen lattices and mediates the reorganization of collagen I fibrils. J. Cell Biol. 1991, 115:1427-1436.
22. Lygoe K.A., Norman J.T., Marshall J.F., et al. Alpha v integrins play an important role in myofibroblast differentiation. Wound Repair Regen. 2004, 12:461-470.
23. Lygoe K.A., Wall I., Stephens P., et al. Role of vitronectin and fibronectin receptors in oral mucosal and dermal myofibroblast differentiation. Biol. Cell 2007, 99:601-614.
24. Masur S.K., Dewal H.S., Dinh T.T., et al. Myofibroblasts differentiate from fibroblasts when plated at low density. Proc. Nat. Acad. Sci. USA. 1996, 93:4219-4223.
25. Tomasek J.J., Haaksma C.J., Eddy R.J., et al. Fibroblast contraction occurs on release of tension in attached collagen lattices: dependency on an organized actin cytoskeleton and serum. Anat. Rec. 1992, 232:359-368.
26. Tomasek J.J., Gabbiani G., Hinz B., et al. Myofibroblasts and mechano-regulation of connective tissue remodelling. Nat. Rev. Mol. Cell Biol. 2002, 3:349-363.
27. von Wichert G., Jiang G., Kostic A., et al. RPTP-alpha acts as a transducer of mechanical force on alphav/beta3-integrin-cytoskeleton linkages. J. Cell Biol. 2003, 161:143-153.
28. Wang Y.L., Pelham R.J. Preparation of a flexible, porous polyacrylamide substrate for mechanical studies of cultured cells. Meth. Enzymol. 1998, 298:489-496.
29. Yeung T., Georges P.C., Flanagan L.A., et al. Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion. Cell Motil. Cytoskeleton 2005, 60:24-34.