Tetracycline-inducible protein expression in pancreatic cancer cells: Effects of CapG overexpression

World Journal of Gastroenterology

Volumn 17, Issue 15, 2011, Pages 1947-1960

  Tonack, Sarah ^a   Patel, Sabina ^a   Jalali, Mehdi ^a   Nedjadi, Taoufk ^a   Jenkins, Rosalind E ^a   Goldring, Christopher ^a   Neoptolemos, John ^a,b   Costello, Eithne ^a  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

^b School of Biomedical Sciences ^*  (United Kingdom)

Author keywords

CapG; Miapaca 2; Panc 1; Pancreatic cancer cells; Suit 2; Tetracycline inducible

Indexed keywords

ACTIN CAPPING PROTEIN; ALPHA TUBULIN; BETA ACTIN; CALCYCLIN; COFILIN; CYTOCHROME P450 2E1; ENOLASE; EZRIN; GENE PRODUCT; GLUTATHIONE TRANSFERASE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; ORNITHINE OXOACID AMINOTRANSFERASE; PROTEIN CAPG; RADIXIN; TETRACYCLINE; TRANSACTIVATOR PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CANCER CELL CULTURE; CELL CYCLE; CELL MOTILITY; CELL NUCLEUS; CELL PROLIFERATION; CHICKEN; CLONE; CONTROLLED STUDY; CYTOMEGALOVIRUS; CYTOPLASM; HUMAN; HUMAN CELL; PANCREAS CANCER; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION;

EID: 79955937047     PISSN: 10079327     EISSN: None     Source Type: Journal    
DOI: 10.3748/wjg.v17.i15.1947     Document Type: Article

References (26)

1. Gossen M, Bujard H. Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc Natl Acad Sci USA 1992; 89: 5547-5551
2. Gossen M, Freundlieb S, Bender G, Müller G, Hillen W, Bujard H. Transcriptional activation by tetracyclines in mammalian cells. Science 1995; 268: 1766-1769
3. Hinrichs W, Kisker C, Düvel M, Müller A, Tovar K, Hillen W, Saenger W. Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance. Science 1994; 264: 418-420
4. Orth P, Cordes F, Schnappinger D, Hillen W, Saenger W, Hinrichs W. Conformational changes of the Tet repressor induced by tetracycline trapping. J Mol Biol 1998; 279: 439-447
5. Urlinger S, Baron U, Thellmann M, Hasan MT, Bujard H, Hillen W. Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity. Proc Natl Acad Sci USA 2000; 97: 7963-7968
6. Welman A, Barraclough J, Dive C. Generation of cells expressing improved doxycycline-regulated reverse transcriptional transactivator rtTA2S-M2. Nat Protoc 2006; 1: 803-811
7. Yu J, Müller H, Hehn S, Koschmieder S, Schönig K, Berdel WE, Serve H, Müller-Tidow C. Construction and application of an inducible system for homogenous expression levels in bulk cell lines. PLoS One 2009; 4: e6445
8. Roth S, Franken P, van Veelen W, Blonden L, Raghoebir L, Beverloo B, van Drunen E, Kuipers EJ, Rottier R, Fodde R, Smits R. Generation of a tightly regulated doxycycline-inducible model for studying mouse intestinal biology. Genesis 2009; 47: 7-13
9. Levy L, Hill CS. Smad4 dependency defines two classes of transforming growth factor {beta} (TGF-{beta}) target genes and distinguishes TGF-{beta}-induced epithelial-mesen-chymal transition from its antiproliferative and migratory responses. Mol Cell Biol 2005; 25: 8108-8125
10. Zhang T, Liu WD, Saunee NA, Breslin MB, Lan MS. Zinc finger transcription factor INSM1 interrupts cyclin D1 and CDK4 binding and induces cell cycle arrest. J Biol Chem 2009; 284: 5574-5581
11. Welman A, Cawthorne C, Barraclough J, Smith N, Griffths GJ, Cowen RL, Williams JC, Stratford IJ, Dive C. Construction and characterization of multiple human colon cancer cell lines for inducibly regulated gene expression. J Cell Biochem 2005; 94: 1148-1162
12. Thompson CC, Ashcroft FJ, Patel S, Saraga G, Vimalachandran D, Prime W, Campbell F, Dodson A, Jenkins RE, Lemoine NR, Crnogorac-Jurcevic T, Yin HL, Costello E. Pancreatic cancer cells overexpress gelsolin family-capping proteins, which contribute to their cell motility. Gut 2007; 56: 95-106
13. Johnston PA, Yu FX, Reynolds GA, Yin HL, Moomaw CR, Slaughter CA, Südhof TC. Purification and expression of gCap39. An intracellular and secreted Ca2(+)-dependent actinbinding protein enriched in mononuclear phagocytes. J Biol Chem 1990; 265: 17946-17952
14. Goldring CE, Kitteringham NR, Jenkins R, Lovatt CA, Randle LE, Abdullah A, Owen A, Liu X, Butler PJ, Williams DP, Metcalfe P, Berens C, Hillen W, Foster B, Simpson A, McLellan L, Park BK. Development of a transactivator in hepatoma cells that allows expression of phase I, phase II, and chemical defense genes. Am J Physiol Cell Physiol 2006; 290: C104-C115
15. Shekouh AR, Thompson CC, Prime W, Campbell F, Hamlett J, Herrington CS, Lemoine NR, Crnogorac-Jurcevic T, Buechler MW, Friess H, Neoptolemos JP, Pennington SR, Costello E. Application of laser capture microdissection combined with two-dimensional electrophoresis for the discovery of differentially regulated proteins in pancreatic ductal adenocarcinoma. Proteomics 2003; 3: 1988-2001
16. Nedjadi T, Kitteringham N, Campbell F, Jenkins RE, Park BK, Navarro P, Ashcroft F, Tepikin A, Neoptolemos JP, Costello E. S100A6 binds to annexin 2 in pancreatic cancer cells and promotes pancreatic cancer cell motility. Br J Cancer 2009; 101: 1145-1154
17. Vimalachandran D, Greenhalf W, Thompson C, Lüttges J, Prime W, Campbell F, Dodson A, Watson R, Crnogorac-Jurcevic T, Lemoine N, Neoptolemos J, Costello E. High nuclear S100A6 (Calcyclin) is signifcantly associated with poor survival in pancreatic cancer patients. Cancer Res 2005; 65: 3218-3225
18. Ermak G, Cancasci VJ, Davies KJ. Cytotoxic effect of doxycycline and its implications for teton gene expression systems. Anal Biochem 2003; 318: 152-154
19. Silacci P, Mazzolai L, Gauci C, Stergiopulos N, Yin HL, Hayoz D. Gelsolin superfamily proteins: key regulators of cellular functions. Cell Mol Life Sci 2004; 61: 2614-2623
20. Witke W, Li W, Kwiatkowski DJ, Southwick FS. Comparisons of CapG and gelsolinnull macrophages: demonstration of a unique role for CapG in receptor-mediated ruffing, phagocytosis, and vesicle rocketing. J Cell Biol 2001; 154: 775-784
21. Iacobuzio-Donahue CA, Maitra A, Olsen M, Lowe AW, van Heek NT, Rosty C, Walter K, Sato N, Parker A, Ashfaq R, Jaffee E, Ryu B, Jones J, Eshleman JR, Yeo CJ, Cameron JL, Kern SE, Hruban RH, Brown PO, Goggins M. Exploration of global gene expression patterns in pancreatic adenocarcinoma using cDNA microarrays. Am J Pathol 2003; 162: 1151-1162
22. Lal A, Lash AE, Altschul SF, Velculescu V, Zhang L, McLendon RE, Marra MA, Prange C, Morin PJ, Polyak K, Papado-poulos N, Vogelstein B, Kinzler KW, Strausberg RL, Riggins GJ. A public database for gene expression in human cancers. Cancer Res 1999; 59: 5403-5407
23. Van den Abbeele A, De Corte V, Van Impe K, Bruyneel E, Boucherie C, Bracke M, Vandekerckhove J, Gettemans J. Downregulation of gelsolin family proteins counteracts cancer cell invasion in vitro. Cancer Lett 2007; 255: 57-70
24. De Corte V, Van Impe K, Bruyneel E, Boucherie C, Mareel M, Vandekerckhove J, Gettemans J. Increased importin-beta-dependent nuclear import of the actin modulating protein CapG promotes cell invasion. J Cell Sci 2004; 117: 5283-5292
25. Hubert T, Van Impe K, Vandekerckhove J, Gettemans J. The actin-capping protein CapG localizes to microtubule-dependent organelles during the cell cycle. Biochem Biophys Res Commun 2009; 380: 166-170
26. Van Impe K, Hubert T, De Corte V, Vanloo B, Boucherie C, Vandekerckhove J, Gettemans J. A new role for nuclear transport factor 2 and Ran: nuclear import of CapG. Traffc 2008; 9: 695-707