Urate oxidase purification by salting-in crystallization: Towards an alternative to chromatography

PLoS ONE

Volumn 6, Issue 5, 2011, Pages

  Giffard, Marion ^a   Ferté, Natalie ^a   Ragot, François ^b   Hajji, Mohamed El ^b   Castro, Bertrand ^b   Bonneté, Françoise ^a  

^a AIX MARSEILLE UNIVERSITÉ   (France)

^b SANOFI   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIUM CHLORIDE; MACROGOL; POLYMER; RASBURICASE; URATE OXIDASE; INORGANIC SALT;

ARTICLE; CALIBRATION; CONTROLLED STUDY; CRYSTALLIZATION; CRYSTALLOGRAPHY; DRUG PURIFICATION; DRUG SOLUBILITY; ENZYME ACTIVITY; FEASIBILITY STUDY; FERMENTATION; GEL FILTRATION; GEL PERMEATION CHROMATOGRAPHY; INTERMETHOD COMPARISON; ISOELECTRIC FOCUSING; MOLECULAR WEIGHT; PH; PROTEIN DEGRADATION; SOLUBILITY; ULTRAFILTRATION; CHEMISTRY; GEL CHROMATOGRAPHY; ISOLATION AND PURIFICATION;

ASPERGILLUS FLAVUS;

CHROMATOGRAPHY, GEL; CRYSTALLIZATION; FERMENTATION; ISOELECTRIC FOCUSING; SALTS; URATE OXIDASE;

EID: 79955888189     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0019013     Document Type: Article

References (39)

1. Judge RA, Johns MR, White ET, (1995) Protein purification by bulk crystallization: the recovery of ovalbumin. Biotechnol Bioeng 48: 316-323.
2. Jacobsen C, Garside J, Hoare M, (1998) Nucleation and growth of microbial lipase crystals from clarified concentrated fermentation broths. Biotechnology and Bioengineering 57: 666-675.
3. Wu XW, Muzny DM, Lee CC, Caskey CT, (1992) Two independent mutational events in the loss of urate oxidase during hominoid evolution. J Mol Evol 34: 78-84.
4. Ames BN, Cathcart R, Schwiers E, Hochstein P, (1981) Uric acid provides an antioxidant defense in humans against oxidant- and radical-caused aging and cancer: a hypothesis. Proc Natl Acad Sci 78: 6858-6862.
5. McGrath B, Walsh G, (2005) Directory of Therapeutic Enzymes. In: McGrath B, Walsh G, editors. CRC Press.
6. Bayol A, Capdevielle J, Malazzi P, Buzy A, Bonnet MC, et al. (2002) Modification of a reactive cysteine explains differences between rasburicase end uricozyme, a natural Aspergillus flavus uricase. Biotechnol Appl Biochem 36: 21-31.
7. Cammalleri L, Malaguarnera M, (2007) Rasburicase represents a new tool for hyperuricemia in tumor lysis syndrome and in gout. Int J Med Sci 4: 83-93.
8. Baker J, (1997) Preparation of stable insulin analog crystals. In: Eli Lilly, editors. Patent 5597893 United States.
9. Faber C, Hobley T, Mollerup J, Thomas ORT, Kaasgaard S, (2007) Study of the Solubility of a Modified Bacillus licheniformis r-Amylase around the Isoelectric Point. J Chemical and Engineering Data 52: 707-713.
10. Alderton G, Fevold H, (1946) Direct crystallization of lysozyme from egg white and some crystalline salts of lysozyme. J Biological Chemistry 146: 1-5.
11. Becker T, Lawlis V, (1991) Subtilisin crystallization process. Patent 5041377 United States.
12. Nielsen N, Nielsen T, (1998) Method for crystallization of enzymes. Patent 5837513 United States.
13. Visuri K, Nummi M, (1972) Purification and characterisation of crystalline-amylase from barley. Eur J Biochem 28: 555-565.
14. Visuri K, Uotila S, Fulton S, Couto D, (2006) Method for crystallization of human serum albumin. United States: GTC-Biotherapeutics. Patent 7087719.
15. Johal S, Lincoln N, (1982) Preparation and crystallization of fraction I protein from plant sources. United States. Patent 4334024.
16. Wagner O, Bauer K, Kaufmann W, Rauenbusch E, Arens A, et al. (1973) Process for the enrichment of L asparaginase. United States: Bayer. Patent 3773624.
17. Weber M, Jones M, Ulrich J, (2008) Crystallization as a purification method for Jack Bean Urease: on the suitability of Poly(Ethylene Glycol), Li2SO4, and NaCl as precipitants. Crystal Growth and Design 8: 711-716.
18. Sumner J, (1926) The isolation and crystallization of the enzyme urease. Journal of Biological Chemistry 69: 435-441.
19. Vivares D, Bonneté F, (2004) Liquid-liquid phase separations in urate oxidase/PEG mixtures: characterization and implications for protein crystallization. J Phys Chem B 108: 6498.
20. Vivares D, Astier J-P, Veesler S, Bonnete F, (2006) Polymorphism of urate oidase in PEG solutions. Crystal Growth & Design 6: 287-292.
21. Giffard M, Delfosse V, Sciara G, Mayer C, Cambillau C, et al. (2009) Surfactant Poloxamer 188 as a New Crystallizing Agent for Urate Oxidase. Crystal Growth and Design 9: 4199-4206.
22. Giffard M, Colloc'h N, Ferté N, Castro C, Bonneté F, (2008) Salting-in effect on urate oxidase crystal design. Crystal Growth and Design 8: 4220-4226.
23. Bonneté F, Vivares D, Robert C, Colloc'h N, (2001) Interactions in solution and crystallization of Aspergillus flavus urate oxidase. J Crystal Growth 232: 330-339.
24. Vivares D, Bonneté F, (2002) X-ray scattering studies of Aspergillus flavus urate oxidase: towards a better understanding of PEG effects on the crystallization of large proteins. Acta Cryst D58: 472-479.
25. Vivares D, Belloni L, Tardieu A, Bonneté F, (2002) Catching the PEG-induced attractive interaction between proteins. Eur Phys J E 9: 15-25.
26. Bonneté F, Finet S, Tardieu A, (1999) Second virial coefficient: variations with lysozyme crystallization conditions. J Crystal Growth 196: 403-414.
27. Budayova M, Bonneté F, Tardieu A, Vachette P, (1999) Interactions in solution of large oligomeric protein. J Crystal Growth 196: 210-219.
28. Tardieu A, Le Verge A, Riès-Kautt M, Malfois M, Bonneté F, et al. (1999) Proteins in solution: from X-ray scattering intensities to interaction potentials. J Crystal Growth 196: 193-203.
29. Malfois M, Bonnete F, Belloni L, Tardieu A, (1996) A model of attractive interactions to account for fluid-fluid phase separation of protein solutions. The Journal of Chemical Physics 105: 3290-3300.
30. Schmolka I, (1977) A review of block polymer surfactants. Journal of the American Oil Chemists' Society 54: 110-116.
31. Aleman C, Bayol A, Breul T, Dupin P, (1998) Stable liquid composition containing urate oxidase and lyophilized composition for its preparation. Patent 5811096 United States: Sanofi, Paris Sanofi, Paris.
32. Fraisse L, Bonnet MC, De Farcy JP, Agut C, Dersigny D, et al. (2002) A colorimetric 96-well microtiter plate assay for the determination of urate oxidase activity and its kinetic parameters. Anal Biochem 309: 173-179.
33. Finet S, Skouri-Panet F, Casselyn M, Bonnete F, Tardieu A, (2004) The Hofmeister effect as seen by SAXS in protein solutions. Current Opinion in Colloid & Interface Science 9: 112-116.
34. Arakawa T, Timasheff SN, (1985) Theory of protein solubility. Methods in Enzymology 114: 49-77.
35. Carbonnaux C, Riès-Kautt M, Ducruix A, (1995) Relative effectiveness of various anions on the solubility of acidic Hypoderma lineatum collagenase at pH 7.2. Protein Sci 4: 2123-2128.
36. Riès-Kautt M, Ducruix A, (1989) Relative effectiveness of various ions on the solubility and crystal growth of lysozyme. J Biol Chem 264: 745-748.
37. Fromme P, Witt HT, (1998) Improved isolation and crystallization of photosystem I for structural analysis. Biochimica et Biophysica Acta (BBA)- Bioenergetics 1365: 175-184.
38. Retailleau P, Ries-Kautt M, Ducruix A, (1997) No salting-in of lysozyme chloride observed at low ionic strength over a large range of pH. Biophys J 73: 2156-2163.
39. Finet S, Vivarès D, Bonneté F, Tardieu A, (2003) Controlling biomolecular crystallization by understanding the distinct effects of PEGs and salts on solubility. Methods in Enzymology. 368: 105-129.