Development and validation of a triplex real-time PCR assay for the rapid detection and differentiation of wild-type and glycoprotein E-deleted vaccine strains of Bovine herpesvirus type 1

Journal of Virological Methods

Volumn 174, Issue 1-2, 2011, Pages 77-84

  Wernike, Kerstin ^a   Hoffmann, Bernd ^a   Kalthoff, Donata ^a   König, Patricia ^a   Beer, Martin ^a  

^a FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

Author keywords

BoHV 1; Bovine herpesvirus type 1; Multiplex PCR; Real time PCR

Indexed keywords

BETA ACTIN; GLYCOPROTEIN D; GLYCOPROTEIN E;

ANIMAL TISSUE; ARTICLE; BOVINE HERPES VIRUS; CATTLE; CELL CULTURE; CONTROLLED STUDY; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; GOLD STANDARD; HERPES VIRUS; INTERMETHOD COMPARISON; NONHUMAN; NOSE SMEAR; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; SEMEN ANALYSIS; SENSITIVITY AND SPECIFICITY; STRAIN DIFFERENCE; TISSUE CULTURE; TRIPLEX REAL TIME POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS INFECTION; VIRUS ISOLATION; VIRUS STRAIN; WILD TYPE;

ACTINS; ANIMALS; CATTLE; CATTLE DISEASES; CLINICAL LABORATORY TECHNIQUES; HERPESVIRIDAE INFECTIONS; HERPESVIRUS 1, BOVINE; POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; SENSITIVITY AND SPECIFICITY; VACCINES, MARKER; VIRAL PROTEINS; VIRAL VACCINES; VIROLOGY;

BOS; BOVINE HERPESVIRUS 1; HERPESVIRIDAE;

EID: 79955758955     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.03.028     Document Type: Article

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