Live Imaging of the Cytoskeleton in Early Cleavage-Stage Zebrafish Embryos

Methods in Cell Biology

Volumn 101, Issue , 2011, Pages 1-18

  Wuhr M ^a   Obholzer, N D ^a   Megason, S G ^a   Detrich, H W ^b   Mitchison, T J ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b NORTHEASTERN UNIVERSITY   (United States)

Author keywords

Cytoskeletal dynamics; Fluorescent protein; Live imaging; Microfilament; Microtubule; Zebrafish embryo

Indexed keywords

EID: 79955603354     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-387036-0.00001-3     Document Type: Book

References (51)

1. Amsterdam A., Hopkins N. Retroviral-mediated insertional mutagenesis in zebrafish. Methods in Cell Biology 2004, Vol. 77:3-20. Elsevier, San Diego.
2. Andersen E., Asuri N., Clay M., Halloran M. Live imaging of cell motility and actin cytoskeleton of individual neurons and neural crest cells in zebrafish embryos. J. Vis. Ex 2010, 36. doi:10.3791/1726.
3. Asakawa K., Kawakami K. A transgenic zebrafish for monitoring in vivo microtubule structures. Dev. Dyn. 2010, 239:2695-2699.
4. Bahary N., Davidson A., Ransom D., Shepard J., Stern H., Trede N., Zhou Y., Barut B., Zon L.I. The Zon laboratory guide to positional cloning in zebrafish. Methods in Cell Biology 2004, Vol. 77:305-329. Elsevier, San Diego.
5. Beis D., Stainier D.Y. In vivo cell biology: following the zebrafish trend. Trends Cell. Biol. 2006, 16:105-112.
6. Betchaku T., Trinkaus J.P. Contact relations, surface activity, and cortical microfilaments of marginal cells of the enveloping layer and of the yolk syncytial and yolk cytoplasmic layers of Fundulus before and during epiboly. J. Exp. Zool. 1978, 206:381-426.
7. Bulinski J.C., Odde D.J., Howell B.J., Salmon T.D., Waterman-Storer C.M. Rapid dynamics of the microtubule binding of ensconsin in vivo. J. Cell Sci. 2001, 114:3885-3897.
8. Burkel B.M., von Dassow G., Bement W.M. Versatile fluorescent probes for actin filaments based on the actin-binding domain of utrophin. Cell Motil. Cytoskeleton 2007, 64:822-832.
9. Corley-Smith G.E., Brandhorst B.P., Walker C., Postlethwait J.H. Production of haploid and diploid androgenetic zebrafish (including methodology for delayed in vitro fertilization). Methods in Cell Biology 1999, Vol. 59:45-60. Elsevier, San Diego.
10. Danilchik M.V., Bedrick S.D., Brown E.E., Ray K. Furrow microtubules and localized exocytosis in cleaving Xenopus laevis embryos. J. Cell Sci. 2003, 116:273-283.
11. Dekens M.P., Pelegri F.J., Maischein H.M., Nüsslein-Volhard C. The maternal-effect gene futile cycle is essential for pronuclear congression and mitotic spindle assembly in the zebrafish zygote. Development 2003, 130:3907-3916.
12. Detrich H.W., Westerfield M., Zon L.I. Overview of the zebrafish system. Methods in Cell Biology 1999, Vol. 59:3-10. Elsevier, San Diego.
13. Driever W., Solnica-Krezel L., Schier A.F., Neuhauss S.C.F., Malicki J., Stemple D.L., Stainier D.Y.R., Zwartkruis F., Abdelilah S., Rangini Z., et al. A genetic screen for mutations affecting embryogenesis in zebrafish. Development 1996, 123:37-46.
14. Faire K., Waterman-Storer C.M., Gruber D., Masson D., Salmon E.D., Bulinski J.C. E-MAP-115 (ensconsin) associates dynamically with microtubules in vivo and is not a physiological modulator of microtubule dynamics. J. Cell Sci. 1999, 112:4243-4255.
15. Haffter P., Granato M., Brand M., Mullins M.C., Hammerschmidt M., Kane D.C., Odenthal J., van Eeden F.J.M., Jiang Y.-J., Heisenberg C.-P., et al. The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio. Development 1996, 123:1-36.
16. Hyman A., Drechsel D., Kellogg D., Salser S., Sawin K., Steffen P., Wordeman L., Mitchison T. Preparation of modified tubulins. Methods Enzymol. 1991, 196:478-485.
17. Jesuthasan S., Stähle U. Dynamic microtubules and specification of the zebrafish embryonic axis. Curr. Biol. 1997, 7:31-42.
18. Kawakami K. Transgenesis and gene trap methods in zebrafish by using the Tol2 transposable element. Methods in Cell Biology 2004, Vol. 77:201-222. Elsevier, San Diego.
19. Knaut H., Pelegri F., Bohmann K., Schwarz H., Nüsslein-Volhard C. Zebrafish vasa RNA but not its protein is a component of the germ plasm and segregates asymmetrically before germline specification. J. Cell Biol. 2000, 149:875-888.
20. Kwan K.M., Fujimoto E., Grabher C., Mangum B.D., Hardy M.E., Campbell D.S., Parant J.M., Yost H.J., Kanki J.P., Chien C.B. The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. Dev. Dyn. 2007, 236:3088-3099.
21. Li W.M., Webb S.E., Chan C.M., Miller A.L. Multiple roles of the furrow deepening Ca2+ transient during cytokinesis in zebrafish embryos. Dev. Biol. 2008, 316:228-248.
22. Li W.M., Webb S.E., Lee K.W., Miller A.L. Recruitment and SNARE-mediated fusion of vesicles in furrow membrane remodeling during cytokinesis in zebrafish embryos. Exp. Cell Res. 2006, 312:3260-3275.
23. Lindeman R.E., Pelegri F. Vertebrate maternal-effect genes: Insights into fertilization, early cleavage divisions, and germ cell determinant localization from studies in the zebrafish. Mol. Reprod. Dev. 2010, 77:299-313.
24. Lister J.A., Robertson C.P., Lepage T., Johnson S.L., Raible D.W. nacre encodes a zebrafish microphthalmia-related protein that regulates neural-crest-derived pigment cell fate. Development 1999, 126:3757-3767.
25. McKinney S.A., Murphy C.S., Hazelwood K.L., Davidson M.W., Looger L.L. A bright and photostable photoconvertible fluorescent protein. Nat. Methods 2009, 6:131-133.
26. Mitchison T.J. Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence. J. Cell Biol. 1989, 109:637-652.
27. Mitchison T., Kirschner M. Dynamic instability of microtubule growth. Nature 1984, 312:237-242.
28. Needleman D.J., Groen A., Ohi R., Maresca T., Mirny L., Mitchison T. Fast microtubule dynamics in meiotic spindles measured by single molecule imaging: evidence that the spindle environment does not stabilize microtubules. Mol. Biol. Cell 2010, 21:323-333.
29. Orkin S.H., Zon L.I. Genetics of erythropoiesis: induced mutations in mice and zebrafish. Annu. Rev. Genet. 1997, 31:33-60.
30. Pelegri F., Mullins M.C. Genetic screens for maternal-effect mutations. Methods in Cell Biology 2004, Vol. 77:21-51. Elsevier, San Diego.
31. Rosenblatt J., Peluso P., Mitchison T.J. The bulk of unpolymerized actin in Xenopus egg extracts is ATP-bound. Mol. Biol. Cell 1995, 6:227-236.
32. Sakai N., Burgess S., Hopkins N. Delayed in vitro fertilization of zebrafish eggs in Hank's saline containing bovine serum albumin. Mol. Mar. Biol. Biotechnol. 1997, 6:84-87.
33. Siripattarapravat K., Busta A., Steibel J.P., Cibelli J. Characterization and in vitro control of MPF activity in zebrafish eggs. Zebrafish 2009, 6:97-105.
34. Skromne I., Prince V.E. Current perspectives in zebrafish reverse genetics: moving forward. Dev. Dyn. 2008, 237:861-882.
35. Solnica-Krezel L., Driever W. Microtubule arrays of the zebrafish yolk cell: organization and function during epiboly. Development 1994, 120:2443-2455.
36. Strähle U., Jesuthasan S. Ultraviolet irradiation impairs epiboly in zebrafish embryos: evidence for a microtubule-dependent mechanism of epiboly. Development 1993, 119:909-919.
37. Strasser M.J., Mackenzie N.C., Dumstrei K., Nakkrasae L.I., Stebler J., Raz E. Control over the morphology and segregation of zebrafish germ cell granules during embryonic development. BMC. Dev. Biol. 2008, 8:58.
38. Taunton J., Rowning B.A., Coughlin M.L., Wu M., Moon R.T., Mitchison T.J., Larabell C.A. Actin-dependent propulsion of endosomes and lysosomes by recruitment of N-WASP. J. Cell Biol. 2000, 148:519-530.
39. Theusch E.V., Brown K.J., Pelegri F. Separate pathways of RNA recruitment lead to the compartmentalization of the zebrafish germ plasm. Dev. Biol. 2006, 292:129-141.
40. Topczewski J., Solnica-Krezel L. Cytoskeletal dynamics of the zebrafish embryo. Essential Zebrafish Methods, Part A: Cell and Developmental Biology 2009, 133-157. Elsevier, San Diego. H.W. Detrich, M. Westerfield, L.I. Zon (Eds.).
41. Trinkaus J.P. The surface gel layer of Fundulus eggs in relation to epiboly. Proc. Natl. Acad. Sci. U. S. A. 1949, 35:218-225.
42. Trinkaus J.P. A study of mechanisms of epiboly in the egg of Fundulus heteroclitus. J. Exp. Zool. 1951, 118:269-319.
43. Urasaki A., Morvan G., Kawakami K. Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition. Genetics 2006, 174:639-649.
44. Velarde N., Gunsalus K.C., Piano F. Diverse roles of actin in C. elegans early embryogenesis. BMC Dev. Biol. 2007, 7:142.
45. Von Dassow G., Verbrugghe K.J., Miller A.L., Sider J.R., Bement W.M. Action at a distance during cytokinesis. J. Cell Biol. 2009, 187:831-845.
46. Westerfield M. 2007, University of Oregon Press, Eugene, Oregon.
47. White R.M., Sessa A., Burke C., Bowman T., LeBlanc J., Ceol C., Bourque C., Dovey M., Goessling W., Burns C.E., et al. Transparent adult zebrafish as a tool for in vivo transplantation analysis. Cell Stem Cell 2008, 2:183-189.
48. Wühr M., Chen Y., Dumont S., Groen A.C., Needleman D.J., Salic A., Mitchison T.J. Evidence for an upper limit to mitotic spindle length. Curr. Biol. 2008, 18:1256-1261.
49. Wühr M., Tan E.S., Parker S.K., Detrich H.W., Mitchison T.J. A model for cleavage plane determination in early amphibian and fish embryos. Curr. Biol. 2010, 20:2040-2045.
50. Yabe T., Ge X., Lindeman R., Nair S., Runke G., Mullins M.C., Pelegri F. The maternal-effect gene cellular island encodes aurora B kinase and is essential for furrow formation in the early zebrafish embryo. PLoS. Genet. 2009, 5. e1000518.
51. Zhai Y., Kronebusch P.J., Simon P.M., Borisy G.G. Microtubule dynamics at the G2/M transition: abrupt breakdown of cytoplasmic microtubules at nuclear envelope breakdown and implications for spindle morphogenesis. J. Cell Biol. 1996, 135:201-214.