Phospholemman (FXYD1) raises the affinity of the human α 1β1 isoform of Na,K-ATPase for Na ions

Biochemistry

Volumn 50, Issue 18, 2011, Pages 3736-3748

  Cirri, Erica ^a   Katz, Adriana ^b   Mishra, Neeraj Kumar ^b   Belogus, Talya ^b   Lifshitz, Yael ^b   Garty, Haim ^b   Karlish, Steven J D ^b   Apell, Hans Jürgen ^a  

^a UNIVERSITY OF KONSTANZ   (Germany)

^b WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE MODE; ATP-BINDING; BINDING AFFINITIES; CONFORMATIONAL TRANSITIONS; ELECTROCHROMICS; EQUILIBRIUM TITRATIONS; FLUORESCENCE METHOD; FUNCTIONAL PROPERTIES; IN-VITRO; INORGANIC PHOSPHATES; ION BINDING; ISOFORMS; KINETIC EXPERIMENT; METAL CHELATE; PARTIAL REACTIONS; PICHIA PASTORIS; SELECTIVE BINDING; TIME CONSTANTS; WHOLE CELL;

BINDING ENERGY; BIOCHEMISTRY; CHELATION; DYES; ESCHERICHIA COLI; EXPERIMENTS; IONS; PHOSPHORYLATION; PURIFICATION; RATE CONSTANTS; TITRATION;

BINDING SITES;

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM) ALPHA1; ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM) BETA1; ADENOSINE TRIPHOSPHATE; ISOPROTEIN; MEMBRANE PROTEIN; PHOSPHATE; PHOSPHOLEMMAN; SODIUM ION; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; ESCHERICHIA COLI; FLUORESCENCE; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION;

ADENOSINE TRIPHOSPHATE; ANIMALS; BINDING SITES; CATTLE; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; IONS; KINETICS; MEMBRANE PROTEINS; PHOSPHOPROTEINS; PICHIA; PROTEIN ISOFORMS; RECOMBINANT PROTEINS; SERUM ALBUMIN; SODIUM; SODIUM-POTASSIUM-EXCHANGING ATPASE; SPECTROMETRY, FLUORESCENCE;

ESCHERICHIA COLI; PICHIA PASTORIS;

EID: 79955573060     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi2001714     Document Type: Article

References (60)

1. Forbush, B., III, Kaplan, J. H., and Hoffman, J. F. (1978) Characterization of a new photoaffinity derivative of ouabain: Labeling of the large polypeptide and of a proteolipid component of the Na,K-ATPase Biochemistry 17, 3667-3676 (Pubitemid 8405399)
2. Sweadner, K. J. and Rael, E. (2000) The FXYD gene family of small ion transport regulators or channels: cDNA sequence, protein signature sequence, and expression Genomics 68, 41-56
3. Cornelius, F. and Mahmmoud, Y. A. (2003) Functional modulation of the sodium pump: the regulatory proteins "Fixit" News Physiol Sci. 18, 119-124 (Pubitemid 36782802)
4. Garty, H. and Karlish, S. J. (2005) FXYD proteins: tissue-specific regulators of the Na,K-ATPase Semin. Nephrol. 25, 304-311 (Pubitemid 41749412)
5. Geering, K. (2005) Function of FXYD proteins, regulators of Na, K-ATPase J. Bioenerg. Biomembr. 37, 387-392 (Pubitemid 43725163)
6. Geering, K. (2006) FXYD proteins: new regulators of Na-K-ATPase Am. J. Physiol. Renal Physiol. 290, F241-F250
7. Presti, C. F., Scott, B. T., and Jones, L. R. (1985) Identification of an endogenous protein kinase C activity and its intrinsic 15-kilodalton substrate in purified canine cardiac sarcolemmal vesicles J. Biol. Chem. 260, 13879-13889 (Pubitemid 16233248)
8. Pretorius, P. J., Pohl, W. G., Smithen, C. S., and Inesi, G. (1969) Structural and functional characterization of dog heart microsomes Circ. Res. 25, 487-499
9. Palmer, C. J., Scott, B. T., and Jones, L. R. (1991) Purification and complete sequence determination of the major plasma membrane substrate for cAMP-dependent protein kinase and protein kinase C in myocardium J. Biol. Chem. 266, 11126-11130 (Pubitemid 21906921)
10. Moorman, J. R., Palmer, C. J., John, J. E., III, Durieux, M. E., and Jones, L. R. (1992) Phospholemman expression induces a hyperpolarization- activated chloride current in Xenopus oocytes J. Biol. Chem. 267, 14551-14554
11. Moorman, J. R., Ackerman, S. J., Kowdley, G. C., Griffin, M. P., Mounsey, J. P., Chen, Z., Cala, S. E., O'Brian, J. J., Szabo, G., and Jones, L. R. (1995) Unitary anion currents through phospholemman channel molecules Nature 377, 737-740
12. 2+ exchange in adult rat cardiac myocytes Am. J. Physiol. Heart Circ. Physiol. 284, H225-H233
13. 2+]i transients in adult rat cardiac myocytes Am. J. Physiol. Heart Circ. Physiol. 286, H1322-H1330
14. Guo, K., Wang, X., Gao, G., Huang, C., Elmslie, K. S., and Peterson, B. Z. (2010) Amino acid substitutions in the FXYD motif enhance phospholemman-induced modulation of cardiac L-type calcium channels Am. J. Physiol. Cell Physiol. 299, C1203-C1211
15. Wang, X., Gao, G., Guo, K., Yarotskyy, V., Huang, C., Elmslie, K. S., and Peterson, B. Z. (2010) Phospholemman modulates the gating of cardiac L-type calcium channels Biophys. J. 98, 1149-1159
16. Crambert, G., Fuzesi, M., Garty, H., Karlish, S., and Geering, K. (2002) Phospholemman (FXYD1) associates with Na,K-ATPase and regulates its transport properties Proc. Natl. Acad. Sci. U. S. A. 99, 11476-11481 (Pubitemid 34920950)
17. Wetzel, R. K. and Sweadner, K. J. (2003) Phospholemman expression in extraglomerular mesangium and afferent arteriole of the juxtaglomerular apparatus Am. J. Physiol. Renal Physiol. 285, F121-F129
18. Feschenko, M. S., Donnet, C., Wetzel, R. K., Asinovski, N. K., Jones, L. R., and Sweadner, K. J. (2003) Phospholemman, a single-span membrane protein, is an accessory protein of Na,K-ATPase in cerebellum and choroid plexus J. Neurosci. 23, 2161-2169 (Pubitemid 36368904)
19. Floyd, R. V., Wray, S., Martin-Vasallo, P., and Mobasheri, A. (2010) Differential cellular expression of FXYD1 (phospholemman) and FXYD2 (gamma subunit of Na, K-ATPase) in normal human tissues: a study using high density human tissue microarrays Ann. Anat. 192, 7-16
20. Despa, S., Bossuyt, J., Han, F., Ginsburg, K. S., Jia, L. G., Kutchai, H., Tucker, A. L., and Bers, D. M. (2005) Phospholemman-phosphorylation mediates the beta-adrenergic effects on Na/K pump function in cardiac myocytes Circ. Res. 97, 252-259 (Pubitemid 41105451)
21. i and inotropic state during β-adrenergic stimulation in mouse ventricular myocytes Circulation 117, 1849-1855 (Pubitemid 351507473)
22. + pump function in cardiac myocytes Circ. Res. 99, 1376-1383 (Pubitemid 44949673)
23. Silverman, B. Z., Fuller, W., Eaton, P., Deng, J., Moorman, J. R., Cheung, J. Y., James, A. F., and Shattock, M. J. (2005) Serine 68 phosphorylation of phospholemman: acute isoform-specific activation of cardiac Na/K ATPase Cardiovasc. Res. 65, 93-103 (Pubitemid 40038482)
24. Bers, D. M. and Despa, S. (2009) Na/K-ATPase - an integral player in the adrenergic fight-or-flight response Trends Cardiovasc. Med. 19, 111-118
25. Jia, L. G., Donnet, C., Bogaev, R. C., Blatt, R. J., McKinney, C. E., Day, K. H., Berr, S. S., Jones, L. R., Moorman, J. R., Sweadner, K. J., and Tucker, A. L. (2005) Hypertrophy, increased ejection fraction, and reduced Na-K-ATPase activity in phospholemman-deficient mice Am. J. Physiol. Heart Circ. Physiol 288, H1982-H1988
26. +-ATPase expressed in Pichia pastoris reveals an essential role of phospholipid-protein interactions J. Biol. Chem. 280, 16610-16618 (Pubitemid 41389117)
27. +-ATPase purified from Pichia pastoris membranes by specific interactions with lipids Biochemistry 46, 12855-12867 (Pubitemid 350060106)
28. +-ATPase. Purification of α1/β1/PLM complexes expressed in Pichia pastoris J. Biol. Chem. 281, 15790-15799 (Pubitemid 43848469)
29. Mishra, N. K., Peleg, Y., Cirri, E., Belogus, T., Lifshitz, Y., Voelker, D. R., Apell, H. J., Garty, H., and Karlish, S. J. (2011) FXYD proteins stabilize Na,K-ATPase: Amplification of specific phosphatidylserine-protein interactions J. Biol. Chem. 286, 9699-9712
30. Karlish, S. J. D. (1980) Characterization of conformational changes in (Na,K) ATPase labeled with fluorescein at the active site J. Bioenerg. Biomembr. 12, 111-136
31. Rephaeli, A., Richards, D., and Karlish, S. J. (1986) Conformational transitions in fluorescein-labeled (Na,K)ATPase reconstituted into phospholipid vesicles J. Biol. Chem. 261, 6248-6254 (Pubitemid 17204129)
32. Bühler, R., Stürmer, W., Apell, H.-J., and Läuger, P. (1991) Charge translocation by the Na,K-pump: I. Kinetics of local field changes studied by time-resolved fluorescence measurements J. Membr. Biol. 121, 141-161
33. Stürmer, W., Bühler, R., Apell, H.-J., and Läuger, P. (1991) Charge translocation by the Na,K-pump: II. Ion binding and release at the extracellular face J. Membr. Biol. 121, 163-176
34. Heyse, S., Wuddel, I., Apell, H.-J., and Stürmer, W. (1994) Partial reactions of the Na,K-ATPase: determination of rate constants J. Gen. Physiol. 104, 197-240 (Pubitemid 24262238)
35. Apell, H.-J., Roudna, M., Corrie, J. E., and Trentham, D. R. (1996) Kinetics of the phosphorylation of Na,K-ATPase by inorganic phosphate detected by a fluorescence method Biochemistry 35, 10922-10930 (Pubitemid 26279675)
36. Schneeberger, A. and Apell, H.-J. (2001) Ion selectivity of the cytoplasmic binding sites of the Na,K-ATPase: II. Competition of various cations J. Membr. Biol. 179, 263-273 (Pubitemid 32165799)
37. 1 state of the Na,K-ATPase? FEBS Lett. 532, 198-202 (Pubitemid 35375888)
38. Habeck, M., Cirri, E., Katz, A., Karlish, S. J., and Apell, H. J. (2009) Investigation of electrogenic partial reactions in detergent-solubilized Na,K-ATPase Biochemistry 48, 9147-9155
39. Lifshitz, Y., Petrovich, E., Haviv, H., Goldshleger, R., Tal, D. M., Garty, H., and Karlish, S. J. (2007) Purification of the human α2 isoform of Na,K-ATPase expressed in Pichia pastoris. Stabilization by lipids and FXYD1 Biochemistry 46, 14937-14950 (Pubitemid 350308885)
40. Schwartz, A. K., Nagano, M., Nakao, M., Lindenmayer, G. E., and Allen, J. C. (1971) The sodium- and potassium-activated adenosine triphosphatase system Meth. Pharmacol. 1, 361-388
41. Rembold, C. M., Ripley, M. L., Meeks, M. K., Geddis, L. M., Kutchai, H. C., Marassi, F. M., Cheung, J. Y., and Moorman, J. R. (2005) Serine 68 phospholemman phosphorylation during forskolin-induced swine carotid artery relaxation J. Vasc. Res. 42, 483-491 (Pubitemid 41527603)
42. 2 conformational changes J. Biol. Chem. 284, 31038-31051
43. Schneeberger, A. and Apell, H.-J. (1999) Ion selectivity of the cytoplasmic binding sites of the Na,K-ATPase: I. Sodium binding is associated with a conformational rearrangement J. Membr. Biol. 168, 221-228 (Pubitemid 29177859)
44. Stürmer, W., Apell, H.-J., Wuddel, I., and Läuger, P. (1989) Conformational transitions and change translocation by the Na,K pump: comparison of optical and electrical transients elicited by ATP-concentration jumps J. Membr. Biol. 110, 67-86 (Pubitemid 19206745)
45. McCray, J. A., Herbette, L., Kihara, T., and Trentham, D. R. (1980) A new approach to time-resolved studies of ATP-requiring biological systems; laser flash photolysis of caged ATP Proc. Natl. Acad. Sci. U. S. A 77, 7237-7241 (Pubitemid 11189448)
46. Deluca, M. and McElroy, M. D. (1978) Purification and properties of firefly luciferase Methods Enzymol. 57, 3-15
47. Alber, T. (2009) Signaling mechanisms of the Mycobacterium tuberculosis receptor Ser/Thr protein kinases Curr. Opin. Struct. Biol. 19, 650-657
48. +-ATPase Nature 403, 898-901 (Pubitemid 30130992)
49. Apell, H. J., Benz, G., and Sauerbrunn, D. (2011) Proton diet for the sodium pump Biochemistry 50, 409-418
50. +-ATPase: α/β/FXYD subunit stoichiometry and cross-linking J. Biol. Chem. 281, 5947-5955 (Pubitemid 43847695)
51. Morth, J. P., Pedersen, B. P., Toustrup-Jensen, M. S., Sorensen, T. L., Petersen, J., Andersen, J. P., Vilsen, B., and Nissen, P. (2007) Crystal structure of the sodium-potassium pump Nature 450, 1043-1049 (Pubitemid 350273618)
52. Shinoda, T., Ogawa, H., Cornelius, F., and Toyoshima, C. (2009) Crystal structure of the sodium-potassium pump at 2.4 Å resolution Nature 459, 446-450
53. Therien, A. G., Goldshleger, R., Karlish, S. J., and Blostein, R. (1997) Tissue-specific distribution and modulatory role of the gamma subunit of the Na,K-ATPase J. Biol. Chem. 272, 32628-32634 (Pubitemid 28011953)
54. Beguin, P., Crambert, G., Guennoun, S., Garty, H., Horisberger, J. D., and Geering, K. (2001) CHIF, a member of the FXYD protein family, is a regulator of Na,K-ATPase distinct from the gamma-subunit EMBO J. 20, 3993-4002 (Pubitemid 32751816)
55. Li, C., Grosdidier, A., Crambert, G., Horisberger, J. D., Michielin, O., and Geering, K. (2004) Structural and functional interaction sites between Na,K-ATPase and FXYD proteins J. Biol. Chem. 279, 38895-38902 (Pubitemid 39296050)
56. Teriete, P., Franzin, C. M., Choi, J., and Marassi, F. M. (2007) Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles Biochemistry 46, 6774-6783 (Pubitemid 46906407)
57. Hughes, E., Whittaker, C. A., Barsukov, I. L., Esmann, M., and Middleton, D. A. (2010) A study of the membrane association and regulatory effect of the phospholemman cytoplasmic domain Biochim. Biophys. Acta 145, 165-173
58. Schulz, S. and Apell, H.-J. (1995) Investigation of ion binding to the cytoplasmic binding sites of the Na,K-pump Eur. Biophys. J. 23, 413-421
59. Wuddel, I. and Apell, H.-J. (1995) Electrogenicity of the sodium transport pathway in the Na,K-ATPase probed by charge-pulse experiments Biophys. J. 69, 909-921
60. Bühler, R. and Apell, H.-J. (1995) Sequential potassium binding at the extracellular side of the Na,K-pump J. Membr. Biol. 145, 165-173