Function of the CysD domain of the gel-forming MUC2 mucin

Biochemical Journal

Volumn 436, Issue 1, 2011, Pages 61-70

  Ambort, Daniel ^a   Van Der Post, Sjoerd ^a   Johansson, Malin E V ^a   MacKenzie, Jenny ^b   Thomsson, Elisabeth ^a   Krengel, Ute ^b   Hansson, Gunnar C ^a  

^a UNIVERSITY OF GOTHENBURG   (Sweden)

^b UNIVERSITY OF OSLO   (Norway)

Author keywords

C mannosylation; Disulfide bonds; Mass spectrometry (MS); Mucus; Non covalent dimer

Indexed keywords

MUCIN 2;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DISULFIDE BOND; GEL; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN GLYCOSYLATION;

AMINO ACID SEQUENCE; ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; DIMERIZATION; DISULFIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOSYLATION; HUMANS; MOLECULAR SEQUENCE DATA; MUCIN-2; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS;

EID: 79955537785     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20102066     Document Type: Article

References (42)

1. Perez-Vilar, J. and Hill, R. L. (1999) The structure and assembly of secreted mucins. J. Biol. Chem. 274, 31751-31754
2. Thornton, D. J., Rousseau, K. and McGuckin, M. A. (2008) Structure and function of the polymeric mucins in airways mucus. Annu. Rev. Physiol. 70, 459-486 (Pubitemid 351738188)
3. Lang, T., Hansson, G. C. and Samuelsson, T. (2007) Gel-forming mucins appeared early in metazoan evolution. Proc. Natl. Acad. Sci. U.S.A. 104, 16209-16214 (Pubitemid 350099386)
4. Gum, J. R., Hicks, J. W., Toribara, N. W., Siddiki, B. and Kim, Y. S. (1994) Molecular cloning of human intestinal mucin (MUC2) cDNA. Identification of the amino terminus and overall sequence similarity to prepro-von Willebrand factor. J. Biol. Chem. 269, 2440-2446
5. Li, D., Gallup, M., Fan, N., Szymkowski, D. E. and Basbaum, C. B. (1998) Cloning of the amino-terminal and 5'-flanking region of the human MUC5AC mucin gene and transcriptional up-regulation by bacterial exoproducts. J. Biol. Chem. 273, 6812-6820 (Pubitemid 28160344)
6. Desseyn, J. L, Aubert, J. P., van Seuningen, I., Porchet, N. and Laine, A. (1997) Genomic organization of the 3' region of the human mucin gene MUC5B. J. Biol. Chem. 272, 16873-16883 (Pubitemid 27289786)
7. Toribara, N. W., Roberton, A. M., Ho, S. B., Kuo, W. L., Gum, E., Hicks, J. W., Gum, Jr, J. R., Byrd, J. C., Siddiki, B. and Kim, Y. S. (1993) Human gastric mucin. Identification of a unique species by expression cloning. J. Biol. Chem. 268, 5879-5885 (Pubitemid 23090922)
8. Zhu, L., Lee, P., Yu, D., Tao, S. and Chen, Y. (2010) Cloning and characterization of human MUC19 gene. Am. J. Respir. Cell Mol. Biol., doi:101165/rcmb.2010-0312OC
9. Bobek, L., Tsai, H., Biesbrock, A. and Levine, M. (1993) Molecular cloning, sequence, and specificity of expression of the gene encoding the low molecular weight human salivary mucin (MUC7). J. Biol. Chem. 268, 20563-20569 (Pubitemid 23278970)
10. Johansson, M. E., Phillipson, M., Petersson, J., Velcich, A., Holm, L. and Hansson, G. C. (2008) The inner of the two Muc2 mucin-dependent mucus layers in colon is devoid of bacteria. Proc. Natl. Acad. Sci. U.S.A. 105, 15064-15069
11. Asker, N., Axelsson, M. A., Olofsson, S. O. and Hansson, G. C. (1998) Dimerization of the human MUC2 mucin in the endoplasmic reticulum is followed by a N-glycosylation- dependent transfer of the mono- and dimers to the Golgi apparatus. J. Biol. Chem. 273, 18857-18863 (Pubitemid 28366272)
12. Lidell, M. E., Johansson, M. E., Morgelin, M., Asker, N., Gum, Jr, J. R., Kim, Y. S. and Hansson, G. C. (2003) The recombinant C-terminus of the human MUC2 mucin forms dimers in Chinese-hamster ovary cells and heterodimers with full-length MUC2 in LS 174T cells. Biochem. J. 372, 335-345 (Pubitemid 36723879)
13. Godl, K., Johansson, M. E., Lidell, M. E., Mörgelin, M., Karlsson, H., Olson, F. J., Gum, Jr, J. R., Kim, Y. S. and Hansson, G. C. (2002) The N terminus of the MUC2 mucin forms trimers that are held together within a trypsin-resistant core fragment. J. Biol. Chem. 277, 47248-47256 (Pubitemid 36163373)
14. Escande, F., Aubert, J. P., Porchet, N. and Buisine, M. P. (2001) Human mucin gene MUC5AC: organization of its 5'-region and central repetitive region. Biochem. J. 358, 763-772 (Pubitemid 32896964)
15. Desseyn, J. L., Guyonnet-Dupérat, V., Porchet, N., Aubert, J. P. and Laine, A. (1997) Human mucin gene MUC5B, the 10.7-kb large central exon encodes various alternate subdomains resulting in a super-repeat. Structural evidence for a 11p15.5 gene family. J. Biol. Chem. 272, 3168-3178
16. Pigny, P., Guyonnet-Duperat, V., Hill, A. S., Pratt, W. S., Galiegue-Zouitina, S., d'Hooge, M. C., Laine, A., Van-Seuningen, I., Degand, P., Gum, J. R. et al. (1996) Human mucin genes assigned to 11p15.5: identification and organization of a cluster of genes. Genomics 38, 340-352 (Pubitemid 27030123)
17. Lorenzo, P., Neame, P., Sommarin, Y. and Heinegård, D. (1998) Cloning and deduced amino acid sequence of a novel cartilage protein (CILP) identifies a proform including a nucleotide pyrophosphohydrolase. J. Biol. Chem. 273, 23469-23475 (Pubitemid 28417538)
18. Spada, F., Steen, H., Troedsson, C., Kallesoe, T., Spriet, E., Mann, M. and Thompson, E. M. (2001) Molecular patterning of the oikoplastic epithelium of the larvacean tunicate Oikopleura dioica. J. Biol. Chem. 276, 20624-20632
19. Löftier, A., Doucey, M. A., Jansson, A. M., Müller, D. R., de Beer, T., Hess, D., Meldal, M., Richter, W. J., Vliegenthart, J. F. and Hofsteenge, J. (1996) Spectroscopic and protein chemical analyses demonstrate the presence of C-mannosylated tryptophan in intact human RNase 2 and its isoforms. Biochemistry 35, 12005-12014 (Pubitemid 26313671)
20. Perez-Vilar, J., Randell, S. H. and Boucher, R. C. (2004) C-Mannosylation of MUC5AC and MUC5B Cyssubdomains. Glycobiology 14, 325-337
21. Büackstroüm, M., Link, T., Olson, F. J., Karlsson, H., Graham, R., Picco, G., Buchell, J., Taylor-Papadimitriou, J. T., Noll, T. and Hansson, G. C. (2003) Recombinant MUC1 mucin with a breast cancer-like O-glycosylation produced in large amounts in Chinese-hamster ovary cells. Biochem. J. 376, 677-686 (Pubitemid 38058066)
22. Stanley, P. (1981) Selection of specific wheat germ agglutinin-resistant (WgaR) phenotypes from Chinese hamster ovary cell populations containing numerous lecR genotypes. Mol. Cell. Biol. 1, 687-696 (Pubitemid 11055766)
23. Baückstroüm, D., Hansson, G. C., Nilsson, O., Johansson, C., Gendler, S. and Lindholm, L. (1991) Purification and characterization of a membrane-bound and a secreted mucin-type glycoprotein carrying the carcinoma-associated sialyl-Lea epitope on distinct core proteins. J. Biol. Chem. 266, 21537-21547 (Pubitemid 121000217)
24. Lümmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
25. Swain, M. and Ross, N. W. (1995) A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate-polyacrylamide gels. Electrophoresis 16, 948-951
26. Towbin, H., Staehelin, T. and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U.S.A. 76, 4350-4354 (Pubitemid 9256559)
27. Schügger, H. and von Jagow, G. (1991) Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. Biochem. 199, 223-231
28. Andersch-Bjürkman, Y., Thomsson, K. A., Holmén Larsson, J. M., Ekerhovd, E. and Hansson, G. C. (2007) Large scale identification of proteins, mucins, and their O-glycosylation in the endocervical mucus during the menstrual cycle. Mol. Cell. Proteomics 6, 708-716 (Pubitemid 46630103)
29. Panchaud, A., Singh, P., Shaffer, S. A. and Goodlett, D. R. (2010) xComb: a cross-linked peptide database approach to protein-protein interaction analysis. J. Proteome Res. 9, 2508-2515
30. Harris, L. J., Larson, S. B., Hasel, K. W. and McPherson, A. (1997) Refined structure of an intact IgG2a monoclonal antibody. Biochemistry 36, 1581-1597 (Pubitemid 27086254)
31. Andrews, P. (1965) The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochem. J. 96, 595-606
32. Alhadeff, J. A. (1978) Gel filtration of sialoglycoproteins. Biochem. J. 173, 315-319 (Pubitemid 8368306)
33. Wang, L. W., Leonhard-Melief, C., Haltiwanger, R. S. and Apte, S. S. (2009) Post-translational modification of thrombospondin type-1 repeats in ADAMTS-like 1/punctin-1 by C-mannosylation of tryptophan. J. Biol. Chem. 284, 30004-300015
34. Krieg, J., Glüsner, W., Vicentini, A., Doucey, M. A., Löffler, A., Hess, D. and Hofsteenge, J. (1997) C-Mannosylation of human RNase 2 is an intracellular process performed by a variety of cultured cells. J. Biol. Chem. 272, 26687-26692 (Pubitemid 27458894)
35. Wu, M. M., Llopis, J., Adams, S., McCaffery, J. M., Kulomaa, M. S., Machen, T. E., Moore, H. P. and Tsien, R. Y. (2000) Organelle pH studies using targeted avidin and fluorescein-biotin. Chem. Biol. 7, 197-209 (Pubitemid 30147204)
36. Demaurex, N., Furuya, W., D'Souza, S., Bonifacino, J. S. and Grinstein, S. (1998) Mechanism of acidification of the trans-Golgi network (TGN). In situ measurements of pH using retrieval of TGN38 and furin from the cell surface. J. Biol. Chem. 273, 2044-2051 (Pubitemid 28069251)
37. Miesenbock, G., De Angelis, D. A. and Rothman, J. E. (1998) Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394, 192-195 (Pubitemid 28325974)
38. Guyton, A. C. and Hall, J. E. (2000) Medical Physiology. WB Saunders, Elsevier, Philadelphia
39. Paz, H. B., Tisdale, A. S., Danjo, Y., Spurr-Michaud, S. J., Argueso, P. and Gipson, I. K. (2003) The role of calcium in mucin packaging within goblet cells. Exp. Eye Res. 77, 69-75 (Pubitemid 36776275)
40. Gum, Jr, J. R., Hicks, J. W., Toribara, N. W., Rothe, E. M., Lagace, R. E. and Kim, Y. S. (1992) The human MUC2 intestinal mucin has cysteine-rich subdomains located both upstream and downstream of its central repetitive region. J. Biol. Chem. 267, 21375-21383
41. Davies, J. R. and Carlstedt, I. (2001) Respiratory tract mucins. Cilia and Mucus: from Development to Respiratory Defense. (Salthe, M., ed.), pp. 167-178, Marcel-Dekker, New York
42. Hollingsworth, M. A. and Swanson, B. J. (2004) Mucins in cancer: protection and control of the cell surface. Nat. Rev. Cancer 4, 45-60 (Pubitemid 38082153)