Efficient generation of gene knockout plasmids for Dictyostelium discoideum using one-step cloning

Genomics

Volumn 97, Issue 5, 2011, Pages 321-325

  Wiegand, Stephan ^a   Kruse, Janis ^a   Gronemann, Sina ^a   Hammann, Christian ^a  

^a DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Dictyostelium discoideum; Gene deletion; PCR; Plasmid; Vector

Indexed keywords

RESTRICTION ENDONUCLEASE;

ARTICLE; CONTROLLED STUDY; DICTYOSTELIUM DISCOIDEUM; GENE CONSTRUCT; GENE INSERTION; GENE SILENCING; GENE VECTOR; HOMOLOGOUS RECOMBINATION; INTERMETHOD COMPARISON; MOLECULAR CLONING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ANIMALS; CLONING, MOLECULAR; DICTYOSTELIUM; GENE DELETION; GENE KNOCKOUT TECHNIQUES; GENETIC VECTORS; PLASMIDS; POLYMERASE CHAIN REACTION;

DICTYOSTELIUM DISCOIDEUM;

EID: 79955522624     PISSN: 08887543     EISSN: 10898646     Source Type: Journal    
DOI: 10.1016/j.ygeno.2011.02.001     Document Type: Article

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