A new duplex real-time RT-PCR assay for sensitive and specific detection of African horse sickness virus

Molecular and Cellular Probes

Volumn 25, Issue 2-3, 2011, Pages 87-93

  Monaco, F ^a   Polci, A ^a   Lelli, R ^a   Pinoni, C ^a   Di Mattia, T ^a   Mbulu, R S ^b   Scacchia, M ^a   Savini, G ^a  

^a ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELL ABRUZZO E DEL MOLISE G CAPORALE   (Italy)

^b Central Veterinary Laboratory Windhoek   (Namibia)

Author keywords

African horse sickness virus; Internal positive control; Real time RT PCR; TaqMan MGB probes

Indexed keywords

PRIMER RNA;

AFRICAN HORSE SICKNESS VIRUS; ARTICLE; DIAGNOSTIC TEST ACCURACY STUDY; GENE AMPLIFICATION; GENE SEQUENCE; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROTYPE; VIRUS STRAIN;

AFRICAN HORSE SICKNESS; AFRICAN HORSE SICKNESS VIRUS; ANIMALS; BASE SEQUENCE; DNA PRIMERS; HORSES; MOLECULAR SEQUENCE DATA; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SEQUENCE HOMOLOGY, NUCLEIC ACID;

AFRICAN HORSESICKNESS VIRUS; EQUIDAE;

EID: 79955484931     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2011.01.006     Document Type: Article

References (28)

1. Holmes I.H. Family Reoviridae. Classification and nomenclature of viruses. Fifth report of the international committee on taxonomy of viruses 1991, 186-199. Springer-Verlag, New York. R.I.B. Francki, C.M. Fauquet, D.L. Knudson, F. Brown (Eds.).
2. Verwoerd D.W., Huismans H., Erasmus B.J. Orbiviruses. Comprehensive virology 1979, vol. 14:285-345. Plenum Press, London. H. Fraenkel-Conrat, R.R. Wagner (Eds.).
3. Spence R.P., Moore N.F., Nuttal P.A. The biochemistry of Orbiviruses. Arch Virol 1984, 82:1-18.
4. Roy P., Mertens P.P.C., Casal I. African horse sickness virus structure. Comp Immunol Microbiol Infect Dis 1994 Aug-Nov, 17(3-4):243-273.
5. Grubman M., Lewis S. Identification and characterization of the structural and non-structural proteins of African horse sickness virus and determination of the genome coding assignments. Virology 1992, 186:444-451.
6. Howell P.G. The isolation and identification of further antigenic types of African horse sickness virus. Onderstepoort J Vet Res 1962, 29:139-149.
7. Coetzer J.A.W., Guthrie A.J. African horse sickness. Infectious diseases of livestock, Southern Africa 2004, 1231-1246. Oxford University Press. J.A.W. Coetzer, R.C. Tustin (Eds.).
8. Barnard B.J.H. Epidemiology of African horse sickness and the role of the zebra in South Africa. Arch Virol 1998, 14:13-19.
9. Mellor P.S., Boned J., Hamblin C., Graham S. Isolations of African horse sickness virus from vector insects made during the 1988 epizootic in Spain. Epidemiol Infect 1990 Oct, 105(2):447-454.
10. OIE Chapter 2.5.1. African horse sickness. Manual of diagnostic tests and vaccines for terrestrial animals 2009, World Organization for Animal Health, Paris.
11. OIE Chapter 1.1.5. Validation and quality control of PCR methods for the diagnosis of infectious disease. Manual of diagnostic tests and vaccines for terrestrial animals 2009, World Organization for Animal Health, Paris.
12. Reed L.J., Muench H. A simple method of estimating fifty percent endpoints. Am J Hygiene 1938, 27:493-497.
13. Stone-Marschat M., Carville A., Skowronek A., Laegreid W.W. Detection of African horse sickness virus by reverse transcription-PCR. J Clin Microbiol 1994, 32(3):697-700.
14. Martin S.W., Meek A.H., Willeberg P. Veterinary epidemiology 1987, IOWA State, University Press, Ames, Iowa, p. 343.
15. Scacchia M., Lelli R., Peccio A., Di Mattia T., Mbulu R.S., Hager A.L., et al. African horse sickness: a description of outbreaks in Namibia. Vet Ital 2009 Apr-Jun, 45(2):255-264. 265-74.
16. Zimmerli U., Herholz C., Schwermer H., Hofmann M., Griot C. African horse sickness and equine encephalosis: must Switzerland get prepared. Schweiz Arch Tierheilkd 2010 Apr, 152(4):165-175.
17. Agüero M., Gómez-Tejedor C., Angeles Cubillo M., Rubio C., Romero E., Jiménez-Clavero A. Real-time fluorogenic reverse transcription polymerase chain reaction assay for detection of African horse sickness virus. J Vet Diagn Invest 2008 May, 20(3):325-328.
18. Fernández-Pinero J., Fernández-Pacheco P., Rodríguez B., Sotelo E., Robles A., Arias M., et al. Rapid and sensitive detection of African horse sickness virus by real-time PCR. Res Vet Sci 2008, 86(2):353-358.
19. Aradaib I.E. PCR detection of African horse sickness virus serogroup based on genome segment three sequence analysis. J Virol Methods 2009, 159:1-5.
20. Rodriguez-Sanchez B., Fernandez-Pinero J., Sailleau C., Zientara S., Belak S., Arias M., et al. Novel gel-based and real-time PCR assays for the improved detection of African horse sickness virus. J Virol Methods 2008, 151:87-94.
21. Quan M., Lourens C.W., Maclachlan N.J., Gardner I.A., Guthrie A.J. Development and optimisation of a duplex real-time reverse transcription quantitative PCR assay targeting the VP7 and NS2 genes of African horse sickness virus. J Virol Methods 2010, 10.1016/j.jviromet.2010.03.009.
22. van Niekerk M., van Staden V., van Dijk A.A., Huismans H. Variation of African horse sickness virus non structural protein NS3 in southern Africa. J Gen Virol 2001, 82:149-158.
23. Kutyavin I.V., Afonina I.A., Mills A., Gorn V.V., Lukhtanov E.A., Belousov E.S., et al. 3'-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures. Nucleic Acids Res 2000, 28(2):655-661.
24. Ballagi-Pordány A., Belák S. The use of mimics as internal standards to avoid false negatives in diagnostic PCR. Mol Cell Probes 1996, 10:159-164.
25. Belák S. Molecular diagnosis of viral diseases, present trends and future aspects. A view from the OIE Collaborating Centre for the Application of Polymerase Chain Reaction Methods for Diagnosis of Viral Diseases in Veterinary Medicine. Vaccine 2007, 25:5444-5452.
26. Mackay I.M., Aarde K.E., Nitsche A. Real-time PCR in virology. Nucleic Acids Res 2002, 30:1292-1305.
27. Hofmann M.A. Construction of an infectious chimeric classical swine fever virus containing the 5' UTR of bovine viral diarrhea virus, and its application as a universal internal positive control in real-time RT-PCR. J Virol Methods 2003, 114:77-90.
28. King D.P., Reid S.M., Hutchings G.H., Grierson S.S., Wilkinson P.J., Dixon L.K., et al. Development of a Taq-Man PCR assay with internal amplification control for the detection of African swine fever virus. J Virol Methods 2003, 107:53-61.