Development and optimisation of a duplex real-time reverse transcription quantitative PCR assay targeting the VP7 and NS2 genes of African horse sickness virus

Journal of Virological Methods

Volumn 167, Issue 1, 2010, Pages 45-52

  Quan, M ^a   Lourens, C W ^a   MacLachlan, N J ^b   Gardner, I A ^b   Guthrie, A J ^a  

^a UNIVERSITY OF PRETORIA   (South Africa)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

African horse sickness virus; Duplex; NS2 gene; Real time quantitative RT PCR; VP7 gene

Indexed keywords

DOUBLE STRANDED RNA; NONSTRUCTURAL PROTEIN 2; PROTEIN VP7;

AFRICAN HORSE SICKNESS VIRUS; ARTICLE; BLOOD SAMPLING; CELL STRAIN BHK; HORSE DISEASE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROTYPE; VIRUS DETECTION; VIRUS INFECTION; VIRUS ISOLATION;

AFRICAN HORSE SICKNESS; AFRICAN HORSE SICKNESS VIRUS; ANIMALS; ANTIGENS, VIRAL; BLOOD; DNA PRIMERS; HORSES; MOLECULAR SEQUENCE DATA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; VIRAL CORE PROTEINS; VIRAL NONSTRUCTURAL PROTEINS;

AFRICAN HORSESICKNESS VIRUS; EQUIDAE; EQUINE ENCEPHALOSIS VIRUS; ORBIVIRUS;

EID: 77953025518     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.03.009     Document Type: Article

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