CYP1A1/1B1 and CYP2A6/2A13 activity is conserved in cultures of differentiated primary human tracheobronchial epithelial cells

Toxicology in Vitro

Volumn 25, Issue 4, 2011, Pages 922-929

  Newland, Nik ^a   Baxter, Andrew ^a   Hewitt, Katherine ^a   Minet, Emmanuel ^a  

^a BRITISH AMERICAN TOBACCO   (United Kingdom)

Author keywords

Bioactivation; Cytochrome P450; Lung; Metabolism

Indexed keywords

CYTOCHROME P450; CYTOCHROME P450 1A1; CYTOCHROME P450 1B1; CYTOCHROME P450 2A13; CYTOCHROME P450 2A6; MESSENGER RNA; UNCLASSIFIED DRUG;

ADULT; ARTICLE; CELL DIFFERENTIATION; CELL METABOLISM; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; ENZYME ACTIVITY; EPITHELIUM CELL; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IN VITRO STUDY; LUNG ALVEOLUS EPITHELIUM; MALE; PROTEIN ANALYSIS; PROTEIN EXPRESSION; TRACHEOBRONCHIAL TREE;

ADULT; BRONCHI; CELL LINE; CELL LINE, TUMOR; CELLS, CULTURED; CYTOCHROME P-450 ENZYME SYSTEM; EPITHELIAL CELLS; HUMANS; LUNG; MALE; MODELS, BIOLOGICAL; TIME FACTORS; TRACHEA; YOUNG ADULT;

NICOTIANA TABACUM;

EID: 79955480322     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2011.02.014     Document Type: Article

References (24)

1. Rodgman, A., Perfetti, T.A., 2008. The Chemical Components of Tobacco and Tobacco Smoke, CRC Press, Florida, USA, p. 1259.
2. Backlund M., Ingelman-Sundberg M. Different structural requirements of the ligand binding domain of the aryl hydrocarbon receptor for high- and low-affinity ligand binding and receptor activation. Mol. Pharmacol. 2004, 65(2):416-425.
3. Beisswenger C., Platz J., Seifart C., Vogelmeier C., Bals R. Exposure of differentiated airway epithelial cells to volatile smoke in vitro. Respiration 2004, 71(4):402-409.
4. Castell J.V., Donato M.T., Gomez-Lechon M.J. Metabolism and bioactivation of toxicants in the lung. The in vitro cellular approach. Exp. Toxicol. Pathol. 2005, 57(Suppl. 1):189-204.
5. Delaporte E., Renton K.W. Cytochrome P4501A1 and cytochrome P4501A2 are downregulated at both transcriptional and post-transcriptional levels by conditions resulting in interferon-alpha/beta induction. Life Sci. 1997, 60(10):787-796.
6. Delescluse C., Lemaire G., de S.G., Rahmani R. Is CYP1A1 induction always related to AHR signaling pathway?. Toxicology 2000, 153(1-3):73-82.
7. Gray T.E., Guzman K., Davis C.W., Abdullah L.H., Nettesheim P. Mucociliary differentiation of serially passaged normal human tracheobronchial epithelial cells. Am. J. Respir. Cell. Mol. Biol. 1996, 14(1):104-112.
8. Haswell L.E., Hewitt K., Thorne D., Richter A., Gaca M.D. Cigarette smoke total particulate matter increases mucous secreting cell numbers in vitro: a potential model of goblet cell hyperplasia. Toxicol. In Vitro 2010, 24(3):981-987.
9. Hecht S.S. Tobacco smoke carcinogens and lung cancer. J. Natl. Cancer Inst. 1999, 91(14):1194-1210.
10. Hukkanen J., Jacob P., Benowitz N.L. Metabolism and disposition kinetics of nicotine. Pharmacol. Rev. 2005, 57(1):79-115.
11. Hukkanen J., Lassila A., Paivarinta K., et al. Induction and regulation of xenobiotic-metabolizing cytochrome P450s in the human A549 lung adenocarcinoma cell line. Am. J. Respir. Cell Mol. Biol. 2000, 22(3):360-366.
12. Hukkanen J., Pelkonen O., Hakkola J., Raunio H. Expression and regulation of xenobiotic-metabolizing Cytochrome P450 (CYP) enzymes in human lung. Crit. Rev. Toxicol. 2002, 32(5):391-411.
13. Lin P., Chang Y.C., Chen C.H., et al. A comparative study on the effects of 2, 3, 7, 8,-tetrachlorodibenzo-p-dioxin polychlorinated biphenyl126 and estrogen in human bronchial epithelial cells1. Toxicol. Appl. Pharmacol. 2004, 195(1):83-91.
14. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 2001, 25(4):402-408.
15. Maunders H., Patwardhan S., Phillips J., Clack A., Richter A. Human bronchial epithelial cell transcriptome: gene expression changes following acute exposure to whole cigarette smoke in vitro. Am. J. Physiol. Lung. Cell Mol. Physiol. 2007, 292(5):L1248-L1256.
16. Nebert D.W., Puga A., Vasiliou V. Role of the Ah receptor and the dioxin-inducible [Ah] gene battery in toxicity, cancer, and signal transduction. Ann. NY Acad. Sci. 1993, 685:624-640.
17. Newland N., Richter A. Agents associated with lung inflammation induce similar responses in NCI-H292 lung epithelial cells. Toxicol. In Vitro 2008, 22(7):1782-1788.
18. Oyama T., Sugio K., Uramoto H., et al. Increased cytochrome P450 and aryl hydrocarbon receptor in bronchial epithelium of heavy smokers with non-small cell lung carcinoma carries a poor prognosis. Front Biosci. 2007, 12:4497-4503.
19. Seree E., Villard P.H., Pascussi J.M., et al. Evidence for a new human CYP1A1 regulation pathway involving PPAR-alpha and 2 PPRE sites. Gastroenterology 2004, 127(5):1436-1445.
20. Tsuchiya Y., Nakajima M., Takagi S., Taniya T., Yokoi T. MicroRNA regulates the expression of human cytochrome P450 1B1. Cancer Res. 2006, 66(18):9090-9098.
21. Vainio H., Wilbourn J. Identification of carcinogens within the IARC monograph program. Scand. J. Work Environ. Health 1992, 18(Suppl. 1):64-73.
22. White R.D., Shea D., Solow A.R., Stegeman J.J. Induction and post-transcriptional suppression of hepatic cytochrome P450 1A1 by 3, 3', 4, 4'-tetrachlorobiphenyl. Biochem. Pharmacol. 1997, 53(7):1029-1040.
23. Zhang J.Y., Wang Y., Prakash C. Xenobiotic-metabolizing enzymes in human lung. Curr. Drug Metab. 2006, 7(8):939-948.
24. Zhang X., D'Agostino J., Wu H., et al. CYP2A13: variable expression and role in human lung microsomal metabolic activation of the tobacco-specific carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. J. Pharmacol. Exp. Ther. 2007, 323(2):570-578.