Reduced cytotoxicity of polyhexamethylene biguanide hydrochloride (PHMB) by egg phosphatidylcholine while maintaining antimicrobial efficacy

Chemico-Biological Interactions

Volumn 190, Issue 2-3, 2011, Pages 171-178

  Müller, Gerald ^a   Kramer, Axel ^a   Schmitt, Jürgen ^b   Harden, Daniela ^b   Koburger, Torsten ^c  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b B Braun Melsungen AG   (Germany)

^c HYGIENE NORD GMBH   (Germany)

Author keywords

Antimicrobial efficacy; Cytotoxicity; Egg phosphatidylcholine; In vitro; Liposomes; PHMB; Polyhexamethylene biguanide

Indexed keywords

COSMOCIL PG; EGG YOLK PHOSPHATIDYLCHOLINE; LIPOSOME; PHOSPHATIDYLCHOLINE; POLY(HEXAMETHYLENEBIGUANIDE); UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBACTERIAL ACTIVITY; AQUEOUS SOLUTION; ARTICLE; CONTROLLED STUDY; DRUG CYTOTOXICITY; DRUG EFFICACY; DRUG STABILITY; EMULSION; FIBROBLAST; MOUSE; NONHUMAN; PARTICLE SIZE; PSEUDOMONAS AERUGINOSA; STAPHYLOCOCCUS AUREUS;

ANIMALS; ANTI-INFECTIVE AGENTS, LOCAL; BIGUANIDES; CELL LINE; LIPOSOMES; MICE; MICROBIAL SENSITIVITY TESTS; OVUM; PHOSPHATIDYLCHOLINES; PSEUDOMONAS AERUGINOSA; STAPHYLOCOCCUS AUREUS;

BOVINAE; MAMMALIA; MURINAE; PSEUDOMONAS AERUGINOSA; STAPHYLOCOCCUS AUREUS;

EID: 79955467680     PISSN: 00092797     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbi.2011.02.024     Document Type: Article

References (39)

1. C. Roth, A.G. Beule, A. Kramer, W. Hosemann, T. Kohlmann, and C. Scharf Response analysis of stimulating efficacy of polihexanide in an in vitro wound model with respiratory ciliary epithelial cells Skin Pharmacol. Physiol. 23 Suppl. 1 2010 35 40
2. N.-O. Hübner, and A. Kramer Review on the efficacy, safety and clinical applications of polihexanide (PHMB), a modern wound antiseptic Skin Pharmacol. Physiol. 23 Suppl. 1 2010 17 27
3. ® peritoneal lavage in experimentally induced peritonitis in the guinea pig Hyg. Med. 33 2008 189 193
4. P. Broxton, P.M. Woodcock, and P. Gilbert Binding of polyhexamethylene biguanides to the cell envelope of Escherichia coli ATCC 8739 Microbios 41 1984 15 22 (Pubitemid 14019602)
5. P. Broxton, P.M. Woodcock, M. Heatley, and P. Gilbert Interaction of some polyhexamethylene biguanides and membrane phospholipids in Escherichia coli J. Appl. Bacteriol. 57 1984 115 125
6. T. Ikeda, S. Tazuke, and M. Watanabe Interaction of biologically active molecules with phospholipids membranes Biochim. Biophys. Acta 735 1984 380 386
7. T. Ikeda, A. Ledwith, C.H. Bamford, and R.A. Hann Interaction of a polymeric biguanide biocide with phospholipids membranes Biochim. Biophys. Acta 769 1984 57 66
8. T. Ikeda, S. Tazuke, and C.H. Bamford Interaction of membrane active biguanides with negatively charged species. A model for their interaction with target sites in microbial membranes J. Chem. Res. 6 1985 180 181
9. T. Ikeda, S. Tazuke, C.H. Bamford, and A. Ledwith Spectroscopic studies on the interaction of polymeric in-chain biguanide biocides with phospholipids membranes Bull. Chem. Soc. Jpn. 58 1985 705 709
10. P. Broxton, P.M. Woodcock, and P. Gilbert A study of the antibacterial activity of some polyhexamethylene biguanides towards Escherichia coli ATCC 8739 J. Appl. Bacteriol. 54 1983 345 353 (Pubitemid 13066052)
11. W.C. McMurray, and W.L. Magee Phospholipid metabolism Ann. Rev. Biochem. 41 1972 129 160
12. H.J. Rogers, H.R. Perkins, and J.B. Ward Microbial Cell Walls and Membranes 1980 Chapman and Hall London
13. C. Ratledge, and S.G. Wilkinson Microbial Lipids vol. 1 1988 Acad. Press London
14. A. Kallenberger, C. Kallenberger, and H. Willenegger Experimental investigations on tissue compatibility of antiseptics Hyg. Med. 10 1991 3 15
15. G. Müller, and A. Kramer Effect of selected wound antiseptics on adult articular cartilage (bovine sesamoid bone) in the presence of Escherichia coli and Staphylococcus aureus J. Orthop. Res. 23 2005 127 133 (Pubitemid 40045086)
16. G. Müller, and A. Kramer Biocompatibility index of antiseptic agents by parallel assessment of antimicrobial activity and cellular cytotoxicity J. Antimicrob. Chemother. 61 2008 1281 1287 (Pubitemid 351753595)
17. N. Ostrowsky Liposome size measurements by photon correlation spectroscopy Chem. Phys. Lipids 64 1993 45 56 (Pubitemid 23290865)
18. P. Gilbert, C. Pemperton, and D.E. Wilkinson Barrier properties of Gram-negative cell envelope towards high molecular weight polyhexamethylene biguanides J. Appl. Bacteriol. 69 1990 585 592 (Pubitemid 20359812)
19. ISO 10993-5, Biological evaluation of medical devices - Part 5, Tests for in vitro cytotoxicity, 2009.
20. K.E. Campbell, D. Keast, G. Woodbury, and P. Houghton Wear time in two hydrocolloid dressings using a noval in-vivo model Wounds 15 2003 40 48 (Pubitemid 36299288)
21. E. Borenfreund, and J. Puerner A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90) J. Tissue Cult. Methods 9 1984 7 9 (Pubitemid 15235618)
22. E. Borenfreund, and J. Puerner Toxicity determined in vitro by morphological alterations and neutral red absorption Toxicol. Lett. 24 1985 119 124 (Pubitemid 15103513)
23. A.P. Wilson Cytotoxicity and viability assays J.R.W. Masters, Animal Cell Culture 2000 University Press Oxford 175 219
24. DIN EN 13727, Chemical disinfectants and antiseptics - quantitative suspension test for the evaluation of bactericidal activity in the medical area - test method and requirements (phase 2, step 1), German version prEN 13727, 2009.
25. DIN EN 1040, Chemical disinfectants and antiseptics - quantitative suspension test for the evaluation of basic bactericidal activity of chemical disinfectants and antiseptics - test method and requirements (phase 1), German version EN 1040, 2005.
26. F.-A. Pitten, H.-P. Werner, and A. Kramer A standardized test to assess the impact of different organic challenges on the antimicrobial activity of antiseptics J. Hosp. Infect. 55 2003 108 115 (Pubitemid 38001962)
27. R. Pankov, T. Markovska, P. Antonov, L. Ivanova, and A. Momchilova The plasma membrane lipid composition affects fusion between cells and model membranes Chem. Biol. Interact. 164 2006 167 173 (Pubitemid 44794494)
28. G. Van Meer, D.R. Voelker, and G.W. Feigenson Membrane lipids: where there are and how they behave Nat. Rev. Mol. Cell Biol. 9 2008 112 124 (Pubitemid 351158912)
29. V.P. Torchilin, and V. Weissig Liposomes. A Practical Approach 2nd ed. 2003 University Press Oxford
30. G.I. Schoefl The ultrastructure of chylomicra and of the particles in an artificial fat emulsion Proc. R. Soc. London Ser. B: Biol. Sci. 169 1968 147 152
31. E. Frenk, and A. Marazzi Neurofibromatosis of von Recklinghausen: a quantitative study of the epidermal keratinocyte and melanocyte populations J. Invest. Dermatol. 83 1984 23 25 (Pubitemid 14080895)
32. D.A. Harrison, C. Joos, and R. Ambrosio Morphology of corneal basal epithelial cells by in vivo slit-scanning confocal microscopy Cornea 22 2003 246 248 (Pubitemid 36359163)
33. M.C. Robson Wound infection: a failure of wound healing caused by an imbalance of bacteria Surg. Clin. North Am. 77 1997 637 650 (Pubitemid 27256038)
34. A.B. Wysocki Evaluating and managing open skin wounds: colonization versus infection AACN Clin. Issues 13 2002 382 397
35. R. Edwards, and K.G. Harding Bacteria and wound healing Curr. Opin. Infect. Dis. 17 2004 91 96 (Pubitemid 38453386)
36. G. McDonell, and A.D. Russell Antiseptics and disinfectants: activity, action, and resistance Clin. Microbiol. Rev. 12 1999 147 179 (Pubitemid 29055958)
37. A. Kramer, O. Assadian, G. Müller, S. Reichwagen, H. Widulle, P Heldt, W. Nürnberg, Octenidine, chlorhexidine, iodine and iodophores, With introductory chapter antisepsis. Reprint from A. Kramer, O. Assadian (Eds.), Wallhäußers Praxis der Sterilisation, Desinfektion, Antiseptik und Konservierung, Thieme, Stuttgart, 2008).
38. G. Hardy, and M. Puzovic Formulation, stability, and administration of parenteral nutrition with new lipid emulsions Nutr. Clin. Pract. 24 2009 616 625
39. D.F. Driscoll, P.-R. Ling, and B.R. Bistrian Physical stability of 20% lipid injectable emulsions via simulated syringe infusion: effects of glass vs plastic product packaging J. Parenter. Enteral Nutr. 31 2007 148 153