A new cloning strategy for generating multiple repeats of a repetitive polypeptide based on non-template PCR

Biotechnology Letters

Volumn 33, Issue 5, 2011, Pages 977-983

  Chu, Hun Su ^a   Ryum, Jung ^b   Park, Seo Young ^b   Kim, Byung Gee ^a   Kim, Dong Myung ^c   Won, Jong In ^b  

^a Seoul National University   (South Korea)

^b Hong Ik University   (South Korea)

^c Chungnam National University   (South Korea)

Author keywords

Elastin like polypeptide (ELP); Length library; Multiple repeats of amino acids; Non template PCR

Indexed keywords

BIOMEDICAL APPLICATIONS; CELL FREE PROTEIN SYNTHESIS; CLONING STRATEGY; ELASTIN-LIKE POLYPEPTIDES; EXPRESSION VECTORS; LENGTH LIBRARY; MULTIPLE REPEATS OF AMINO ACIDS; NON-TEMPLATE PCR; POTENTIAL CAPACITY; PROTEIN POLYMERS; REPETITIVE PATTERN; SYNTHETIC GENES;

AMINO ACIDS; CLONING; FUNCTIONAL POLYMERS; GENE ENCODING; GENE EXPRESSION; GLYCOPROTEINS;

POLYPEPTIDES;

BIOMATERIAL; PEPTIDE;

AMINO ACID SEQUENCE; ARTICLE; BIOTECHNOLOGY; GENETICS; METABOLISM; METHODOLOGY; MOLECULAR CLONING; POLYMERASE CHAIN REACTION;

BIOCOMPATIBLE MATERIALS; BIOTECHNOLOGY; CLONING, MOLECULAR; PEPTIDES; POLYMERASE CHAIN REACTION; REPETITIVE SEQUENCES, AMINO ACID;

EID: 79955116574     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0510-7     Document Type: Article

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