In-gel detection of L-amino acid oxidases based on the visualisation of hydrogen peroxide production

Journal of Microbiological Methods

Volumn 85, Issue 3, 2011, Pages 228-229

  Rau, Jan Erik ^a   Fischer, Ulrich ^a  

^a UNIVERSITY OF BREMEN   (Germany)

Author keywords

Enzymatic assay; Hydrogen peroxide; L Amino acid oxidase; Non denaturing SDS PAGE

Indexed keywords

AMINO ACID OXIDASE; HYDROGEN PEROXIDE;

ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SPECIFICITY; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PSEUDOALTEROMONAS; PSEUDOALTEROMONAS FLAVIPULCHRA; REPRODUCIBILITY; SENSITIVITY ANALYSIS;

AMINO ACIDS; BACTERIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROGEN PEROXIDE; L-AMINO ACID OXIDASE; OXIDATION-REDUCTION;

BACTERIA (MICROORGANISMS);

EID: 79955101542     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2011.03.002     Document Type: Article

References (20)

1. Ahn M.Y., Ryu K.S., Lee Y.W., Kim Y. Cytotoxicity and L-amino acid oxidase activity of crude insect drugs. Arch. Pharm. Res. 2000, 23:477-481.
2. Alves R.M., Antonucci G.A., Paiva H.H., Cintra A.C.O., Franco J.J., Mendonça-Franqueiro E.P., Dorta D.J., Giglio J.R., Rosa J.C., Fuly A.L., Dias-Baruffi M. Evidence of caspase-mediated apoptosis induced by L-amino acid oxidase isolated from Bothrops atrox snake venom. Comp. Biochem. Physiol. Mol. Integr. Physiol. 2008, 151:542-550.
3. Chen W.M., Lin C.Y., Sheu S.Y. Investigating antimicrobial activity in Rheinheimera sp. due to hydrogen peroxide generated by L-lysine oxidase activity. Enzyme Microb. Technol. 2010, 46:487-493.
4. Chen W.M., Lin C.Y., Chen C.A., Wang J.T., Sheu S.Y. Involvement of an L-amino acid oxidase in the activity of the marine bacterium Pseudoalteromonas flavipulchra against methicillin-resistant Staphylococcus aureus. Enzyme Microb. Technol. 2010, 47:52-58.
5. Du X.Y., Clemetson K.J. Snake venom L-amino acid oxidases. Toxicon 2002, 40:659-665.
6. Gómez D., Espinosa E., Bertazzo M., Lucas-Elío P., Solano F., Sanchez-Amat A. The macromolecule with antimicrobial activity synthesized by Pseudoalteromonas luteoviolacea strains is an L-amino acid oxidase. Appl. Microbiol. Biotechnol. 2008, 79:925-930.
7. Fujisawa S., Hori K., Miyazawa K., Ito K. Occurrence of L-amino acid oxidase in the marine red alga Gymnogongrus flabelliformis. Bull. Jap. Soc. Sci. Fish. 1982, 48:97-103.
8. Kang D., Gho Y.S., Suh M., Kang C. Highly sensitive and fast protein detection with Coomassie brilliant blue in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Bull. Korean Chem. Soc. 2002, 23:1511-1512.
9. Kasai K., Ishikawa T., Komata T., Fukuchi K., Chiba M., Nozaka H., Nakamura T., Sato T., Miura T. Novel L-amino acid oxidase with antibacterial activity against methicillin-resistant Staphylococcus aureus isolated from epidermal mucus of the flounder Platichthys stellatus. FEBS J. 2010, 277:453-465.
10. Kusakabe H., Kodama K., Kuninaka A., Yoshino H., Misono H., Soda K. A new antitumor enzyme. L-lysine α-oxidase from Trichoderma viride. Purification and enzymological properties. J. Biol. Chem. 1980, 255:976-981.
11. Laemmli U.K. Cleavage of structural proteins during assembly of head of bacteriophage T4. Nature 1970, 227:680-685.
12. Lucas-Elío P., Gómez D., Solano F., Sanchez-Amat A. The antimicrobial activity of marinocine, synthesized by Marinomonas mediterranea, is due to hydrogen peroxide generated by its lysine oxidase activity. J. Bacteriol. 2006, 188:2493-2501.
13. Parikh J.R., Greenstein J.P., Winitz M., Birnbaum S.M. The use of amino acid oxidases for the small-scale preparation of the optical isomers of amino acids. J. Am. Chem. Soc. 1958, 80:953-958.
14. Pistorius E.K., Voss H. Some properties of a basic L-amino acid oxidase from Anacystis nidulans. Biochim. Biophys. Acta 1980, 611:227-240.
15. Stiles B.G., Sexton F.W., Weinstein S.A. Antibacterial effects of different snake venoms: purification and characterization of antibacterial proteins from Pseudechis australis (Australian king brown or Mulga snake) venom. Toxicon 1991, 29:1129-1141.
16. Sun Y.P., Nonobe E., Kobayashi Y., Kuraishi T., Aoki F., Yamamoto K., Sakai S. Characterization and expression of L-amino acid oxidase of mouse milk. J. Biol. Chem. 2002, 277:19080-19086.
17. Wellner D., Meister A. Studies on the mechanism of action of L-amino acid oxidase. J. Biol. Chem. 1961, 236:2357-2364.
18. Takahashi E., Furui M., Seko H., Shibatani T. D-Methionine preparation from racemic methionines by Proteus vulgaris IAM 12003 with asymmetric degrading activity. Appl. Microbiol. Biotechnol. 1997, 47:173-179.
19. Tong H., Chen W., Shi W., Qi F., Dong X. SO-LAAO, a novel L-amino acid oxidase that enables Streptococcus oligofermentans to outcompete Streptococcus mutans by generating H2O2 from peptone. J. Bacteriol. 2008, 190:4716-4721.
20. Yang H., Johnson P.M., Ko K.C., Kamio M., Germann M.W., Derby C.D., Phang C.T. Cloning, characterization, and expression of escapin, a broadly antimicrobial FAD-containing L-amino acid oxidase from ink of the sea hare Aplysia californica. J. Exp. Biol. 2005, 208:3609-3622.