Scanning and negative-staining electron microscopy of protoplast regeneration of a wild-type and two chitin synthase mutants in the pathogenic yeast Candida glabrata

Journal of Electron Microscopy

Volumn 60, Issue 2, 2011, Pages 157-165

  Namiki, Yuichi ^a   Ueno, Keigo ^a   Mitani, Hiroki ^a   Virtudazo, Eric V ^a   Ohkusu, Misako ^a   Shimizu, Kiminori ^a   Kawamoto, Susumu ^a   Chibana, Hiroji ^a   Yamaguchi, Masashi ^a  

^a CHIBA UNIVERSITY   (Japan)

Author keywords

Candida glabrata; chitin synthase; glucan fibril; mutant; protoplast regeneration; scanning electron microscopy

Indexed keywords

CHITIN SYNTHASE; FUNGAL PROTEIN; GLUCAN;

ARTICLE; CANDIDA GLABRATA; CELL WALL; ELECTRON MICROSCOPY; ENZYMOLOGY; FIBER; GENETICS; METABOLISM; MUTATION; PATHOGENICITY; PHYSIOLOGY; PROTOPLAST; SCANNING ELECTRON MICROSCOPY; STAINING; ULTRASTRUCTURE;

CANDIDA GLABRATA; CELL WALL; CHITIN SYNTHASE; FUNGAL PROTEINS; GLUCANS; MICROFIBRILS; MICROSCOPY, ELECTRON; MICROSCOPY, ELECTRON, SCANNING; MUTATION; NEGATIVE STAINING; PROTOPLASTS;

CANDIDA GLABRATA; SACCHAROMYCES CEREVISIAE;

CANDIDA; CHITIN; GRANULAR MATERIALS; YEAST;

CANDIDA GLABRATA; CHITIN SYNTHASE; GLUCAN FIBRIL; GLUCANS; MUTANT; MUTANT STRAIN; NEGATIVE-STAINING ELECTRON MICROSCOPY; PROTOPLAST REGENERATION; WILD TYPES; WILD-TYPE STRAIN;

SCANNING ELECTRON MICROSCOPY;

EID: 79955045675     PISSN: 00220744     EISSN: 14779986     Source Type: Journal    
DOI: 10.1093/jmicro/dfq082     Document Type: Article

References (15)

1. Fidel P L Jr, Vazquez J A, and Sobel J D (1999) Candida glabrata: review of epidemiology, pathogenesis, and clinical disease with comparison to C. Albicans. Clin. Microbiol. Rev. 12: 80-96. (Pubitemid 29055954)
2. Dujon B, Sherman D, Fischer G, Durrens P, Casaregola S, Lafontaine I, De Montigny J, Marck C, Neuveglise C, et al. (2004). Genome evolution in yeasts. Nature 430: 35-44. (Pubitemid 38915217)
3. Ueno K, Uno J, Nakayama H, Sasamoto K, Mikami Y, and Chibana H (2007) Development of a highly efficient gene targeting system induced by transient repression of YKU80 expression in Candida glabrata. Eukaryotic Cell 6: 1239-1247. (Pubitemid 47077175)
4. Aguilar-Uscanga B and François J M (2003) A study of the yeast cell wall composition and structure in response to growth conditions and mode of cultivation. Lett. Appl. Microbiol. 37: 268-274. (Pubitemid 37041227)
5. Saxena I M, Brown R M, Fevre M, Geremia R A, and Henrissat B (1995) Multidomain architecture of beta-glycosyl transferases: implications for mechanism of action. J. Bacteriol. 177: 1419-1424.
6. Valdivieso M H, Mol P C, Shaw J A, Cabib E, and Durán A (1991) CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae. J. Cell Biol. 114: 101-109. (Pubitemid 21909784)
7. Yamaguchi M, Okada H, and Namiki Y (2009) Smart specimen preparation for freeze-substitution and serial ultrathin sectioning of yeast cells. J. Electron Microsc. 58: 261-266.
8. Yamaguchi M, Shimizu M, Yamaguchi T, Ohkusu M, and Kawamoto S (2005) Repeated use of uranyl acetate solution for section staining in transmission electron microscopy. Plant Morphol. 17: 57-59.
9. Yamaguchi M, Ikeda R, Nishimura M, and Kawamoto S (2010) Localization by scanning immunoelectron microscopy of triosephosphate isomerase, the molecules responsible for contact-mediated killing of Cryptococcus, on the surface of Staphylococcus. Microbiol. Immunol. 54: 368-370.
10. Yamaguchi M, Sugahara K, Schiosaki K, Mizokami H, and Takeo K (1998) Fine structure of hepatitis B virus surface antigen produced by recombinant yeast: comparison with HBsAg of human origin. FEMS Microbiol. Lett. 165: 363-367. (Pubitemid 29090319)
11. Konomi M, Ishiguro J, and Osumi M (2000) Abnormal formation of the glucan network from regenerating protoplasts in Schizosaccharomyces pombe cps8 actin point mutant. J. Electron Microsc. 49: 569-578.
12. Nishiyama Y, Aoki Y, and Yamaguchi H (1995) Morphological aspects of cell wall formation during protoplast regeneration in Candida albicans. J. Electron Microsc. 44: 72-78.
13. Necǎs O and Svoboda A (1981) Morphogenesis in protoplasts. In: Arnold W N (ed.), Yeast Cell Envelopes: Biochemistry, Biophysics, and Ultrastructure, Vol II, pp 105-127 (CRC Press, Florida).
14. Osumi M, Yamada N, Kobori H, Taki A, Naito N, Baba M, and Nagatani T (1989) Cell wall formation in regenerating protoplasts of Schizosaccharomyces pombe: study by high resolution, low voltage scanning electron microscopy. J. Electron Microsc. 38: 457-468. (Pubitemid 20120124)
15. Osumi M, Sato M, Ishijima S A, Konomi M, Takagi T, and Yaguchi H (1998) Dynamics of cell wall formation in fission yeast, Schizosaccharomyces pombe. Fungal Genet. Biol. 24: 178-206. (Pubitemid 28472947)