Anion exchange chromatography of 4.2 kbp plasmid based vaccine (pcDNA3F) from alkaline lysed E. coli lysate using amino functionalised polymethacrylate conical monolith

Separation and Purification Technology

Volumn 78, Issue 3, 2011, Pages 303-310

  Ongkudon, Clarence M ^a   Danquah, Michael K ^a  

^a MONASH UNIVERSITY   (Australia)

Author keywords

Anion exchange chromatography; Conical column; Plasmid; Polymethacrylate monolith

Indexed keywords

ANION EXCHANGE; ANION EXCHANGE CHROMATOGRAPHY; CONICAL COLUMN; DIETHYLAMINES; E. COLI; HUMAN POPULATION; NACL CONCENTRATION; NEW STRATEGY; PH CONDITION; PLASMID; PLASMID RECOVERY; PLASMID-BASED VACCINES; POLYMETHACRYLATES; TRIETHYLAMINES; VACCINE PRODUCTION;

ALKALINITY; ESCHERICHIA COLI; FUNCTIONAL GROUPS; IONS; MANUFACTURE; MONOLITHIC INTEGRATED CIRCUITS; PURIFICATION; RNA; SODIUM CHLORIDE; VACCINES;

COLUMN CHROMATOGRAPHY;

ESCHERICHIA COLI;

EID: 79954582751     PISSN: 13835866     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.seppur.2011.01.039     Document Type: Article

References (31)

1. H. Huber, W. Buchinger, J. Diewok, R. Ganja, D. Keller, J. Urthaler, and R. Necina Industrial Manufacturing of Plasmid DNA: Boehringer's New cGMP Production System Employs Prudent Vector Design as a Backbone 2008 Genetic Engineering & Biotechnology News USA pp. 4-6
2. M Przybylowski, S. Bartido, O. Borquez-Ojeda, M. Sadelain, and I. Riviere Production of clinical-grade plasmid DNA for human Phase I clinical trials and large animal clinical studies Vaccine 25 2007 5013 5024 (Pubitemid 46879997)
3. K.J. Prather, S. Sagar, J. Murphy, and M. Chartrain Industrial scale production of plasmid DNA for vaccine and gene therapy: plasmid design, production, and purification Enzyme Microb. Technol. 33 2003 865 883 (Pubitemid 37412544)
4. C.M. Ongkudon, and M.K. Danquah Mitigating the looming vaccine crisis: production and delivery of plasmid vaccines Crit. Rev. Biotechnol. 2010 10.3109/07388551.2010.483460
5. M.S. Levy, R.D. O'Kennedy, P. Ayazi-Shamlou, and P. Dunnill Biochemical engineering approaches to the challenges of producing pure plasmid DNA Trends Biotechnol. 18 2000 296 305 (Pubitemid 30387052)
6. J. Stadler, R. Lemmens, and T. Nyhammar Plasmid DNA purification J. Gene Med. 6 2004 S54 S66
7. J. Urthaler, W. Buchinger, and R. Necina Improved downstream process for the production of plasmid DNA for gene therapy Acta Biochim. Pol. 52 2005 703 711 (Pubitemid 41571718)
8. J. Urthaler, R. Schlegl, A. Podgornik, A. Strancar, A. Jungbauer, and R. Necina Application of monoliths for plasmid DNA purification development and transfer to production J. Chromatogr. A 1065 2005 93 106 (Pubitemid 40247841)
9. M.W. Roberts, C.M. Ongkudon, G.M. Forde, and M.K. Danquah Versatility of polymethacrylate monoliths for chromatographic purification of biomolecules J. Sep. Sci. 32 2009 2485 2494
10. A. Zochling, R. Hahn, K. Ahrer, J. Urthaler, and A. Jungbauer Mass transfer characteristics of plasmids in monoliths J. Sep. Sci. 27 2004 819 827 (Pubitemid 39136468)
11. I. Ali, F.E.O. Suliman, and H.Y. Aboul-Enein Superficially porous and monolithic columns: tools for ultra-fast separations in HPLC LC-GC 27 2009 22 33
12. M.K. Danquah, and G.M. Forde Preparation of macroporous methacrylate monolithic material with convective flow properties for bioseparation: investigating the kinetics of pore formation and hydrodynamic performance Chem. Eng. J. 140 2008 593 599
13. I. Ali, V.D. Gaitonde, and H.Y. Aboul-Enein Monolithic silica stationary phases in liquid chromatography J. Chromatogr. Sci. 47 2009 432 442
14. F. Smrekar, A. Podgornik, M. Ciringer, S. Kontrec, P. Raspor, A. Strancar, and M. Peterka Preparation of pharmaceutical-grade plasmid DNA using methacrylate monolithic columns Vaccine 28 2010 2039 2045
15. N.L. Krajnc, F. Smrekar, J. Cerne, P. Raspor, M. Modic, D. Krgovic, A. Strancar, and A. Podgornik Purification of large plasmids with methacrylate monolithic columns J. Sep. Sci. 32 2009 2682 2690
16. F. Svec, and A.A. Kurganov Less common applications of monoliths. III. Gas chromatography J. Chromatogr. A 1184 2008 281 295 (Pubitemid 351296210)
17. J. Vidic, A. Podgornik, and A. Strancar Effect of the glass surface modification on the strength of methacrylate monolith attachment J. Chromatogr. A 1065 2005 51 58 (Pubitemid 40247836)
18. W. Pfeiffer Converging flow chromatography in constant-pressure mode J. Chromatogr. A 1006 2003 149 170 (Pubitemid 36897672)
19. A. Pecavar, A. Smidovnik, and M. Prosek Determination of conical column performances using acyclovir in human plasma sample Anal. Sci. 13 1997 229 234 (Pubitemid 127691226)
20. A. Pecavar, A. Smidovnik, and M. Prosek Conically shaped chromatographic column Anal. Sci. 15 1999 233 240
21. C.M. Ongkudon, and M.K. Danquah Process optimisation for anion exchange monolithic chromatography of 4.2 kbp plasmid vaccine (pcDNA3F) J. Chromatogr. B 878 2010 2719 2725
22. W. Kopaciewicz, M.A. Rounds, and F.E. Regnier Stationary phase contribution to retention in high-performance anion-exchange protein chromatography ligand density and mixed mode effects J. Chromatogr. A 318 1985 16
23. D.L. Wu, and R.R. Walters Effects of stationary phase ligand density on highperformance ion-exchange chromatography of proteins J. Chromatogr. A 598 1992 7 13
24. Y. Huang, J. Bi, W. Zhou, Y. Li, Y. Wang, G. Ma, and Z. Su Improving recovery of recombinant hepatitis B virus surface antigen by ion-exchange chromatographic supports with low ligand density Process Biochem. 41 2006 2320 2326 (Pubitemid 44486082)
25. M.K. Danquah, J. Ho, and G.M. Forde Performance of R-N(R9)-R99 functionalised poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) monolithic sorbent for plasmid DNA adsorption J. Sep. Sci. 30 2007 2843 2850 (Pubitemid 350238314)
26. W. Yan, and L. Huang The effects of salt on the physicochemical properties and immunogenicity of protein based vaccine formulated in cationic liposome Int. J. Pharm. 368 2009 56 62
27. N. Lendero, J. Vidic, P. Brne, A. Podgornik, and A. Strancar Simple method for determining the amount of ion-exchange groups on chromatographic supports J. Chromatogr. A 1065 2005 29 38 (Pubitemid 40247833)
28. C.P. Bisjak, R. Bakry, C.W. Huck, and G.K. Bonn Amino-functionalized monolithic poly(glycidyl methacrylate-codivinylbenzene) ion-exchange stationary phases for the separation of oligonucleotides Chromatographia 62 2005 S31 S36
29. M.B. Gorbet, and M.V. Sefton Endotoxin: the uninvited guest Biomaterials 26 2005 6811 6817 (Pubitemid 41095506)
30. M.K. Danquah, and G.M. Forde Towards the design of a scalable and commercially viable technique for plasmid purification using a methacrylate monolithic stationary phase J. Chem. Technol. Biotechnol. 82 2007 752 757 (Pubitemid 47272344)
31. M.K. Danquah, and G.M. Forde The suitability of DEAE-Cl active groups on customized poly(GMA-co-EDMA) continuous stationary phase for fast enzyme-free isolation of plasmid DNA J. Chromatogr. B 853 2007 38 46 (Pubitemid 46891334)