Detection of fatty acid ethyl esters in skin surface lipids as biomarkers of ethanol consumption in alcoholics, social drinkers, light drinkers, and teetotalers using a methodology based on microwave-assisted extraction followed by solid-phase microextraction and gas chromatography-mass spectrometry

Journal of Analytical Toxicology

Volumn 35, Issue 4, 2011, Pages 232-237

  González Illán, Fernando ^a   Ojeda Torres, Geovannie ^a   Díaz Vázquez, Liz M ^a   Rosario, Osvaldo ^a  

^a UNIVERSITY OF PUERTO RICO   (Puerto Rico)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL; FATTY ACID ESTER; MYRISTIC ACID ETHYL ESTER; OLEIC ACID ETHYL ESTER; PALMITIC ACID ETHYL ESTER; SKIN LIPID; STEARIC ACID ETHYL ESTER; UNCLASSIFIED DRUG;

ADULT; ALCOHOL ABSTINENCE; ALCOHOL CONSUMPTION; ALCOHOLISM; ARTICLE; DRINKING BEHAVIOR; FEMALE; HUMAN; LIMIT OF DETECTION; LIMIT OF QUANTITATION; MALE; MASS FRAGMENTOGRAPHY; SEBUM; SOLID PHASE MICROEXTRACTION;

EID: 79954572313     PISSN: 01464760     EISSN: 01464760     Source Type: Journal    
DOI: 10.1093/anatox/35.4.232     Document Type: Article

References (22)

1. J.P. Allen and R.Z. Litten. The role of laboratory testing in alcoholism treatment. J. Subst. Abuse Treat. 20: 81-85 (2001).
2. NHTSA's National Center for Statistics and Analysis. http://www.nhtsa.gov (accessed September 2009).
3. Office of National Drug control Policy, P.R. Profile of Drug Indicators March 2007. https://www.whitehousedrugpolice.gov (accessed June 2009).
4. G. Schmitt, P. Droenner, G. Skopp, and R. Aderjan. Ethylglucoronide concentration in serum of human volunteers, teetotalers, and suspected drunken drivers. J. Forensic Sci. 42: 1099-1102 (1997).
5. P. Hanson, M. Caron, G. Johnson, L.A. Gustavsson, and C. Alling. Blood phosphatidylethanol as a marker of alcohol abuse: levels in alcoholic males during withdrawal. Alcohol Clin. Exp. Res. 21: 108-110 (1997).
6. M. Laposata. Fatty acid ethyl esters: short-term and long-term serum markers of ethanol intake. Clin. Chem. 43: 1527-1534 (1997).
7. M.A.B. Diczfalusy, I. Björkhem, C. Einarsson, C.G. Hillerbrant, and S.H.E. Alexson. Characterization of enzymes involved in the formation of ethyl esters of long chain fatty acids in humans. J. Lipid Res. 42: 1025-1032 (2001).
8. K.M. Doyle, J.E. Cluette-Brown, D.M. Dube, T.G. Bernhardt, C.R. Morse, and M. Laposata. Fatty acid ethyl esters in the blood as markers of ethanol intake. J. Am. Med. Assoc. 276: 1152-1156 (1996).
9. S. Hartwig, V. Auwärter, and F. Pragst. Fatty acid ethyl esters in scalp, pubic, axillary, beard and body hair as markers for alcohol misuse. Alcohol Alcohol. 38(2): 163-167 (2003).
10. M. Laposata. Fatty acid ethyl esters: ethanol metabolites which mediate ethanol-induce organ damage and serve as markers of ethanol intake. Prog. Lipid Res. 37: 301-316 (1998).
11. V. Auwärter, F. Sporkert, S. Hartwig, F. Pragst, H. Vater, and A. Diefenbacher. Fatty acid ethyl esters in hair as markers of alcohol consumption. Segmental hair analysis of alcoholics, social drinkers, and teetotalers. Clin. Chem. 47: 2114-2123 (2001).
12. F. Pragst,V. Auwärter, F. Sporkert, and K. Spiegel. Analysis of fatty acid ethyl esters in hair as possible markers of chronically elevated alcohol consumption by headspace solid-phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). Forensic Sci. Int. 121: 76-88 (2001).
13. F.M. Wurst, S. Alexson, M. Wolfersdorf, G. Bechtel, S. Forster, C. Alling, S. Aradóttir, K. Jachau, P. Huber, J.P. Allen,V. Auwärter, and F. Pragst. Concentration of FAEE in hair of alcoholics: comparison to other biological state markers and self reported-ethanol intake. Alcohol Alcohol. 39(1): 33-38 (2004).
14. M. Laposata. Fatty acid ethyl esters: current facts and speculations. Prostaglandins Leukot. Essent. Fatty Acids 60: 313-315 (1999).
15. F. Pragst, V. Auwärter, B. Kieβling, and C. Dyes. Wipe-test and patch-test for alcohol misuse based on the concentration ratio of fatty acid ethyl esters and squalene CFAEE/CSQ in skin surface lipids. Forensic Sci. Int. 143(2): 77-86 (2004).
16. L.M. Díaz-Vázquez, O. Garcia, Z. Velázquez, I. Marrero, and O. Rosario. Optimization of microwave-assisted extraction followed by solid phase micro extraction and gas chromatography-mass spectrometry detection for the assay of some semi volatile organic pollutants in sebum. J. Chromatogr. B 825: 11-20 (2005).
17. J. Serup. Formation of oiliness and sebum output-comparison of a lipid-absorbant and occlusive-tape method with photometry. Clin. Exp. Dermatol. 16: 258-263 (1991).
18. Alcohol consumption definition: Alcohol and public health. http://www.cdc.gov/alcohol/faqs.htm#6 (accessed January 2010).
19. W.H. Organization. Lexicon of alcohol and drug terms. http://www.who.int/substance_abuse/terminology/who_lexicon/en/index.html (accessed January 2010).
20. G.E. Piérard, C. Piérard-Franchimont, T. Lê, and C. Lapière. Patterns of follicular sebum excretion rate during lifetime. Arch. Dermatol. Res. 279: 104-107 (1987).
21. A.N. Thody and S. Shuster. Control and function of sebaceous glands. Physiol. Rev. 69: 383-416 (1989).
22. D.T. Downing, M.L. Steward, P.W. Wertz, S.W. Colton, W. Abraham, and J.S. Strauss. Skin lipids: an update. J. Invest. Derm. 88(3 Suppl): 2s-6s (1987).