메뉴 건너뛰기




Volumn 5, Issue 8, 2010, Pages 1470-1479

In vitro culture and expansion of human limbal epithelial cells

Author keywords

[No Author keywords available]

Indexed keywords

RECOMBINANT ENZYME; RECOMBINANT GROWTH FACTOR; TRYPSIN;

EID: 77955179585     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2010.115     Document Type: Article
Times cited : (95)

References (31)
  • 1
    • 0022470546 scopus 로고
    • Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells
    • Schermer, A., Galvin, S. & Sun, T.T. Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J. Cell. Biol. 103, 49-62 (1986).
    • (1986) J. Cell. Biol. , vol.103 , pp. 49-62
    • Schermer, A.1    Galvin, S.2    Sun, T.T.3
  • 2
    • 0024523631 scopus 로고
    • Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: Implications on epithelial stem cells
    • Cotsarelis, G., Cheng, S.Z., Dong, G., Sun, T.T. & Lavker, R.M. Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: implications on epithelial stem cells. Cell 57, 201-209 (1989).
    • (1989) Cell , vol.57 , pp. 201-209
    • Cotsarelis, G.1    Cheng, S.Z.2    Dong, G.3    Sun, T.T.4    Lavker, R.M.5
  • 3
    • 0026025784 scopus 로고
    • Corneal epithelial wound healing in the absence of limbal epithelium
    • Huang, A.J. & Tseng, S.C. Corneal epithelial wound healing in the absence of limbal epithelium. Invest. Ophthalmol. Vis. Sci. 32, 96-105 (1991).
    • (1991) Invest. Ophthalmol. Vis. Sci. , vol.32 , pp. 96-105
    • Huang, A.J.1    Tseng, S.C.2
  • 4
    • 0028834486 scopus 로고
    • Cytologic evidence of corneal diseases with limbal stem cell defciency
    • Puangsricharern, V. & Tseng, S.C. Cytologic evidence of corneal diseases with limbal stem cell defciency. Ophthalmology 102, 1476-1485 (1995).
    • (1995) Ophthalmology , vol.102 , pp. 1476-1485
    • Puangsricharern, V.1    Tseng, S.C.2
  • 6
    • 0031453106 scopus 로고    scopus 로고
    • Amniotic membrane transplantation for ocular surface reconstruction in patients with chemical and thermal burns
    • Shimazaki, J., Yang, H.Y. & Tsubota, K. Amniotic membrane transplantation for ocular surface reconstruction in patients with chemical and thermal burns. Ophthalmology 104, 2068-2076 (1997).
    • (1997) Ophthalmology , vol.104 , pp. 2068-2076
    • Shimazaki, J.1    Yang, H.Y.2    Tsubota, K.3
  • 7
    • 0024523084 scopus 로고
    • Limbal autograft transplantation for ocular surface disorders
    • Kenyon, K.R. & Tseng, S.C. Limbal autograft transplantation for ocular surface disorders. Ophthalmology 96, 709-722 (1989).
    • (1989) Ophthalmology , vol.96 , pp. 709-722
    • Kenyon, K.R.1    Tseng, S.C.2
  • 8
    • 0028148031 scopus 로고
    • Human allograft limbal transplantation for corneal surface reconstruction
    • Tsai, R.J. & Tseng, S.C. Human allograft limbal transplantation for corneal surface reconstruction. Cornea 13, 389-400 (1994).
    • (1994) Cornea , vol.13 , pp. 389-400
    • Tsai, R.J.1    Tseng, S.C.2
  • 9
    • 0030896153 scopus 로고    scopus 로고
    • Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium
    • Pellegrini, G., Traverso, C.E., Franzi, A.T., Zingirian, M., Cancedda, R. & De Luca, M. Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. Lancet 349, 990-993 (1997).
    • (1997) Lancet , vol.349 , pp. 990-993
    • Pellegrini, G.1    Traverso, C.E.2    Franzi, A.T.3    Zingirian, M.4    Cancedda, R.5    De Luca, M.6
  • 10
    • 0034644186 scopus 로고    scopus 로고
    • Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells
    • Tsai, R.J., Li, L.M. & Chen, J.K. Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells. N. Engl. J. Med. 343, 86-93 (2000).
    • (2000) N. Engl. J. Med. , vol.343 , pp. 86-93
    • Tsai, R.J.1    Li, L.M.2    Chen, J.K.3
  • 11
    • 0033911272 scopus 로고    scopus 로고
    • Successful transplantation of bioengineered tissue replacements in patients with ocular surface disease
    • Schwab, I.R., Reyes, M. & Isseroff, R.R. Successful transplantation of bioengineered tissue replacements in patients with ocular surface disease. Cornea 19, 421-426 (2000).
    • (2000) Cornea , vol.19 , pp. 421-426
    • Schwab, I.R.1    Reyes, M.2    Isseroff, R.R.3
  • 12
    • 0038312073 scopus 로고    scopus 로고
    • Use of autologous cultured limbal and conjunctival epithelium in a patient with severe bilateral ocular surface disease induced by acid injury: A case report of unique application
    • Sangwan, V.S., Vemuganti, G.K., Iftekhar, G., Bansal, A.K. & Rao, G.N. Use of autologous cultured limbal and conjunctival epithelium in a patient with severe bilateral ocular surface disease induced by acid injury: a case report of unique application. Cornea 22, 478-481 (2003).
    • (2003) Cornea , vol.22 , pp. 478-481
    • Sangwan, V.S.1    Vemuganti, G.K.2    Iftekhar, G.3    Bansal, A.K.4    Rao, G.N.5
  • 13
    • 33644846229 scopus 로고    scopus 로고
    • Clinical outcome of autologous cultivated limbal epithelium transplantation
    • Sangwan, V.S. et al. Clinical outcome of autologous cultivated limbal epithelium transplantation. Indian J. Ophthalmol. 54, 29-34 (2006).
    • (2006) Indian J. Ophthalmol. , vol.54 , pp. 29-34
    • Sangwan, V.S.1
  • 14
    • 33845254086 scopus 로고    scopus 로고
    • Technique of cultivating limbal derived corneal epithelium on human amniotic membrane for clinical transplantation
    • Fatima, A. et al. Technique of cultivating limbal derived corneal epithelium on human amniotic membrane for clinical transplantation. J. Postgrad. Med. 52, 257-261 (2006).
    • (2006) J. Postgrad. Med. , vol.52 , pp. 257-261
    • Fatima, A.1
  • 16
    • 3142770462 scopus 로고    scopus 로고
    • Ex-vivo potential of cadaveric and fresh limbal tissues to regenerate cultured epithelium
    • Vemuganti, G.K., Kashyap, S., Sangwan, V.S. & Singh, S. Ex-vivo potential of cadaveric and fresh limbal tissues to regenerate cultured epithelium. Indian J. Ophthalmol. 52, 113-120 (2004).
    • (2004) Indian J. Ophthalmol. , vol.52 , pp. 113-120
    • Vemuganti, G.K.1    Kashyap, S.2    Sangwan, V.S.3    Singh, S.4
  • 17
    • 0035890901 scopus 로고    scopus 로고
    • Autologous fbrin-cultured limbal stem cells permanently restore the corneal surface of patients with total limbal stem cell defciency
    • Rama, P. et al. Autologous fbrin-cultured limbal stem cells permanently restore the corneal surface of patients with total limbal stem cell defciency. Transplantation 72, 1478-1485 (2001).
    • (2001) Transplantation , vol.72 , pp. 1478-1485
    • Rama, P.1
  • 18
    • 61549142420 scopus 로고    scopus 로고
    • Myogel supports the ex-vivo amplifcation of corneal epithelial cells
    • Francis, D., Abberton, K., Thompson, E. & Daniell, M. Myogel supports the ex-vivo amplifcation of corneal epithelial cells. Exp. Eye Res. 88, 339-346 (2009).
    • (2009) Exp. Eye Res. , vol.88 , pp. 339-346
    • Francis, D.1    Abberton, K.2    Thompson, E.3    Daniell, M.4
  • 19
    • 73349110142 scopus 로고    scopus 로고
    • Development of a surface-modifed contact lens for the transfer of cultured limbal epithelial cells to the cornea for ocular surface diseases
    • Deshpande, P., Notara, M., Bullett, N., Daniels, J.T., Haddow, D.B. & MacNeil, S. Development of a surface-modifed contact lens for the transfer of cultured limbal epithelial cells to the cornea for ocular surface diseases. Tissue Eng. Part A 15, 2889-2902 (2009).
    • (2009) Tissue Eng. Part A , vol.15 , pp. 2889-2902
    • Deshpande, P.1    Notara, M.2    Bullett, N.3    Daniels, J.T.4    Haddow, D.B.5    MacNeil, S.6
  • 20
    • 53149144307 scopus 로고    scopus 로고
    • Tissue-engineered recombinant human collagen-based corneal substitutes for implantation: Performance of type i versus type III collagen
    • Merrett, K. et al. Tissue-engineered recombinant human collagen-based corneal substitutes for implantation: performance of type I versus type III collagen. Invest. Ophthalmol. Vis. Sci. 49, 3887-3894 (2008).
    • (2008) Invest. Ophthalmol. Vis. Sci. , vol.49 , pp. 3887-3894
    • Merrett, K.1
  • 21
    • 33644870465 scopus 로고    scopus 로고
    • Transplantation of tissue-engineered epithelial cell sheets after excimer laser photoablation reduces postoperative corneal haze
    • Hayashida, Y. et al. Transplantation of tissue-engineered epithelial cell sheets after excimer laser photoablation reduces postoperative corneal haze. Invest. Ophthalmol. Vis. Sci. 47, 552-557 (2006).
    • (2006) Invest. Ophthalmol. Vis. Sci. , vol.47 , pp. 552-557
    • Hayashida, Y.1
  • 22
    • 38849193297 scopus 로고    scopus 로고
    • Culture and characterization of oral mucosal epithelial cells on human amniotic membrane for ocular surface reconstruction
    • Madhira, S.L., Vemuganti, G., Bhaduri, A., Gaddipati, S., Sangwan, V.S. & Ghanekar, Y. Culture and characterization of oral mucosal epithelial cells on human amniotic membrane for ocular surface reconstruction. Mol. Vis. 14, 189-196 (2008).
    • (2008) Mol. Vis. , vol.14 , pp. 189-196
    • Madhira, S.L.1    Vemuganti, G.2    Bhaduri, A.3    Gaddipati, S.4    Sangwan, V.S.5    Ghanekar, Y.6
  • 23
    • 33751011892 scopus 로고    scopus 로고
    • Autologous serum-derived cultivated oral epithelial transplants for severe ocular surface disease
    • Ang, L.P. et al. Autologous serum-derived cultivated oral epithelial transplants for severe ocular surface disease. Arch. Ophthalmol. 124, 1543-1551 (2006).
    • (2006) Arch. Ophthalmol. , vol.124 , pp. 1543-1551
    • Ang, L.P.1
  • 24
    • 33748956316 scopus 로고    scopus 로고
    • Transplantation of autologous serum-derived cultivated corneal epithelial equivalents for the treatment of severe ocular surface disease
    • Nakamura, T. et al. Transplantation of autologous serum-derived cultivated corneal epithelial equivalents for the treatment of severe ocular surface disease. Ophthalmology 113, 1765-1772 (2006).
    • (2006) Ophthalmology , vol.113 , pp. 1765-1772
    • Nakamura, T.1
  • 25
    • 33645388655 scopus 로고    scopus 로고
    • The use of autologous serum in the development of corneal and oral epithelial equivalents in patients with Stevens-Johnson syndrome
    • Nakamura, T. et al. The use of autologous serum in the development of corneal and oral epithelial equivalents in patients with Stevens-Johnson syndrome. Invest. Ophthalmol. Vis. Sci. 47, 909-916 (2006).
    • (2006) Invest. Ophthalmol. Vis. Sci. , vol.47 , pp. 909-916
    • Nakamura, T.1
  • 26
    • 2942530677 scopus 로고    scopus 로고
    • Phenotypic characterization of human corneal epithelial cells expanded ex vivo from limbal explant and single cell cultures
    • Kim, H.S., Jun Song, X., de Paiva, C.S., Chen, Z., Pfugfelder, S.C. & Li, D.Q. Phenotypic characterization of human corneal epithelial cells expanded ex vivo from limbal explant and single cell cultures. Exp. Eye Res. 79, 41-49 (2004).
    • (2004) Exp. Eye Res. , vol.79 , pp. 41-49
    • Kim, H.S.1    Jun Song, X.2    De Paiva, C.S.3    Chen, Z.4    Pfugfelder, S.C.5    Li, D.Q.6
  • 27
    • 0032927810 scopus 로고    scopus 로고
    • Suppression of transforming growth factor-beta isoforms, TGF-beta receptor type II, and myofbroblast differentiation in cultured human corneal and limbal fbroblasts by amniotic membrane matrix
    • Tseng, S.C., Li, D.Q. & Ma, X. Suppression of transforming growth factor-beta isoforms, TGF-beta receptor type II, and myofbroblast differentiation in cultured human corneal and limbal fbroblasts by amniotic membrane matrix. J. Cell. Physiol. 179, 325-335 (1999).
    • (1999) J. Cell. Physiol. , vol.179 , pp. 325-335
    • Tseng, S.C.1    Li, D.Q.2    Ma, X.3
  • 29
    • 48649096374 scopus 로고    scopus 로고
    • A putative role for RHAMM/HMMR as a negative marker of stem cell-containing population of human limbal epithelial cells
    • Ahmad, S. et al. A putative role for RHAMM/HMMR as a negative marker of stem cell-containing population of human limbal epithelial cells. Stem Cells 26, 1609-1619 (2008).
    • (2008) Stem Cells , vol.26 , pp. 1609-1619
    • Ahmad, S.1
  • 30
    • 0033577809 scopus 로고    scopus 로고
    • Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface
    • Pellegrini, G. et al. Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface. J. Cell. Biol. 145, 769-782 (1999).
    • (1999) J. Cell. Biol. , vol.145 , pp. 769-782
    • Pellegrini, G.1
  • 31
    • 77954394144 scopus 로고    scopus 로고
    • Limbal stem-cell therapy and long-term corneal regeneration
    • doi: 10.1056/NEJMoa0905955 (23 June 2010)
    • Rama, P. et al. Limbal stem-cell therapy and long-term corneal regeneration. N. Engl. J. Med. published online, doi: 10.1056/NEJMoa0905955 (23 June 2010).
    • N. Engl. J. Med. Published Online
    • Rama, P.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.