Identification of pythium insidiosum by nested PCR in cutaneous lesions of Brazilian horses and rabbits

Current Microbiology

Volumn 62, Issue 4, 2011, Pages 1225-1229

  Botton, Sonia A ^a   Pereira, Daniela I B ^b   Costa, Mateus M ^c   Azevedo, Maria Isabel ^a   Argenta, Juliana S ^a   Jesus, Francielli P K ^a   Alves, Sydney Hartz ^a   Santurio, Janio Morais ^a  

^a FEDERAL UNIVERSITY OF SANTA MARIA   (Brazil)

^b FEDERAL UNIVERSITY OF PELOTAS   (Brazil)

^c UNIVERSIDADE FEDERAL DO VALE DO SÃO FRANCISCO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL DNA; INTERNAL TRANSCRIBED SPACER; INTERNAL TRANSCRIBED SPACER 1; INTERNAL TRANSCRIBED SPACER 2; INTERNAL TRANSCRIBED SPACER 4; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC TEST; DNA DETERMINATION; DNA EXTRACTION; DNA STRUCTURE; FUNGUS IDENTIFICATION; GENE AMPLIFICATION; HORSE; NESTED POLYMERASE CHAIN REACTION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PYTHIOSIS; PYTHIUM; PYTHIUM INSIDIOSUM; RABBIT; SKIN DEFECT;

ANIMALS; BRAZIL; HORSE DISEASES; HORSES; POLYMERASE CHAIN REACTION; PYTHIOSIS; PYTHIUM; RABBITS;

ANIMALIA; EQUIDAE; FUNGI; ORYCTOLAGUS CUNICULUS; PYTHIUM INSIDIOSUM;

EID: 79954416297     PISSN: 03438651     EISSN: 14320991     Source Type: Journal    
DOI: 10.1007/s00284-010-9781-4     Document Type: Article

References (23)

1. Alexopoulos CJ, Mims CW, Blackwell M (1996). Fungal systematics. In: Introductory mycology. Wiley, New York, pp 683-737
2. SMG Bosco GM Reis RC Theodoro, et al. 2008 Morphological and molecular characterization o fan equine isolate of Pythium insidiosum and comparison with the first human isolate from the same geographic region Med Mycol 46 557 565 10.1080/13693780801975014
3. Groff ACM (2005) PCR para o diagnóstico da campilobacteriose genital bovina. Dissertação apresentada ao Curso de Mestrado do Programa de Pós-graduação em Medicina Veterinária, Área de concentração Medicina Veterinária Preventiva, Universidade Federal de Santa Maria (UFSM, RS), Santa Maria, RS, 39 pp (thesis in Portuguese)
4. AM Grooters MK Gee 2002 Development of a nested polymerase chain reaction assay for the detection and identification of Pythium insidiosum J Vet Intern Med 16 147 152 11899029 (Pubitemid 135746937)
5. L Mendoza L Kaufman PG Standard 1986 Immunodiffusion test for diagnosing and monitoring pythiosis in horses J Clin Microbiol 23 813 816 3086368 1:STN:280:DyaL283isVCrtA%3D%3D
6. L Mendoza L Kaufman PG Standard 1987 Antigenic relationship between the animal and human pathogen Pythium insidiosum and nonpathogenic Pythium species J Clin Microbiol 25 2159 2162 3121666 1:STN:280:DyaL1c%2FoslyhsQ%3D%3D (Pubitemid 17151525)
7. L Mendoza V Nicholson JF Prescott 1992 Immunoblot analysis of the humoral immune response to Pythium insidiosum in horses with pythiosis J Clin Microbiol 30 2980 2983 1452669 1:STN:280:DyaK3s%2FosVOksw%3D%3D
8. L Mendoza L Kaufmann W Mandy, et al. 1997 Serodiagnosis of human and animal pythiosis using an enzyme-linked immunosorbent assay Clin Diagn Lab Immunol 4 715 718 9384295 1:STN:280:DyaK1c%2Fktl2msw%3D%3D (Pubitemid 27501412)
9. RI Miller RSF Campbell 1982 Immunological studies on equine phycomycosis Aust Vet J 58 227 231 6814414 10.1111/j.1751-0813.1982.tb00682.x 1:STN:280:DyaL3s%2FkvVSqsQ%3D%3D
10. RI Miller RSF Campbell 1983 Experimental pythiosis in rabbits Sabouraudia 21 331 341 6658627 10.1080/00362178385380481 1:STN:280:DyaL2c%2Fpsl2gsQ%3D%3D (Pubitemid 14211113)
11. Murray P, Baron EJ, Pfaller MA et al (1995) Manual of clinical microbiology. ASM Press, Washington, p 1482
12. Pereira DIB (2008) Suscetibilidade in vitro e in vivo de Pythium insidiosum: estudo comparativo entre acetato de caspofungina e imunoterapia em coelhos. Tese de Doutorado n. 86 (Especialidade: Micologia). Programa de Pós-Graduação em Ciências Veterinárias. www.ufrgs.br/ppgcv. Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, 117 pp (thesis in Portuguese)
13. PA Pesavento B Barr SM Riggs, et al. 2008 Cutaneous pythiosis in a nestling white-faced ibis Vet Pathol 45 538 541 18587102 10.1354/vp.45-4-538 1:STN:280:DC%2BD1cvhslyltw%3D%3D
14. Santurio JM, Ferreiro L (2008) Pitiose: Uma Abordagem Micológica e Terapêutica. Porto Alegre, Ed. da Universidade Federal do Rio Grande do Sul, 111 pp
15. JM Santurio AT Leal AB Leal, et al. 2003 Three types of immunotherapics against pythiosis insidiosi developed and evaluated Vaccine 21 2535 2540 12744888 10.1016/S0264-410X(03)00035-5 1:STN:280:DC%2BD3s3isFWrsg%3D%3D (Pubitemid 36561346)
16. AM Schurcho L Mendoza AWAM de Cock, et al. 2004 Development of a species-specific probe for Pythium insidiosum and the diagnosis of pythiosis J Clin Microbiol 42 2411 2418 10.1128/JCM.42.6.2411-2418.2004 (Pubitemid 38747482)
17. AM Schurko L Mendoza AWAM de Cock, et al. 2003 Evidence for geographic clusters: molecular genetic differences among strains of Pythium insidiosum from Asia, Australia, and the Americas are explored Mycologia 95 200 208 21156606 10.2307/3762031 1:CAS:528:DC%2BD3sXktlGksbc%3D (Pubitemid 36557819)
18. J Supabandhu MC Fisher Mendoza, et al. 2007 Isolation and identification of the human pathogen Pythium insidiosum from environmental samples collected in Thai agricultural areas Med Mycol 46 41 52 10.1080/13693780701513840 (Pubitemid 351298240)
19. NO Villa K Kageyama T Asano, et al. 2006 Phylogenetic relationships of Pythium and Phytophthora species based on ITS rDNA, cytochrome oxidase II and β-tubulin gene sequences Mycologia 98 410 422 17040070 10.3852/mycologia. 98.3.410 1:CAS:528:DC%2BD28XhtFamsLrO (Pubitemid 44440917)
20. PH Wang JG White 1997 Molecular characterization of Pythium species based on RFLP analysis of the internal transcribed spacer region of ribosomal DNA Physiol Mol Plant Pathol 51 129 143 10.1006/pmpp.1997.0109 1:CAS:528: DyaK1cXhvVOntw%3D%3D (Pubitemid 28031038)
21. White TJ, Burns T, Lee S et al (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315-322
22. D Zhang Y Yang LA Castlebury, et al. 1996 A method for the large scale isolation of high transformation FEMS Microbiol Lett 11 261 265 10.1111/j.1574-6968.1996.tb08587.x (Pubitemid 26395425)
23. N Znajda A Grooters R Marsella 2002 PCR-based detection of Pythium and Lagenidium DNA in frozen and ethanol-fixed animal tissues Vet Dermatol 13 187 194 12229858 10.1046/j.1365-3164.2002.00296.x (Pubitemid 135712164)