Morphological and molecular characterization of an equine isolate of Pythium insidiosumand comparison with the first human isolate from the same geographic region

Medical Mycology

Volumn 46, Issue 6, 2008, Pages 557-565

  De Moraes Gimenes Bosco, Sandra ^a   Martins Reis, Gabriela ^a   Cordeiro Theodoro, Raquel ^a   Assis Da Graca Macoris, Severino ^a   Alencar Marques, Silvio ^a   Da Graca Macoris, Delphim ^a   Bagagli, Eduardo ^a  

^a SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

Author keywords

ITS 5.8S rDNA; Pythiosis; Pythium insidiosum; Sporangia; Zoospores

Indexed keywords

AMPHOTERICIN B; ANTIFUNGAL AGENT; GLUCOSE; RIBOSOME DNA; SODIUM IODIDE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BRAZIL; CONTROLLED STUDY; CULTURE MEDIUM; DNA SEQUENCE; FUNGAL MORPHOLOGY; FUNGUS CULTURE; FUNGUS IDENTIFICATION; FUNGUS ISOLATION; GENOTYPE; GEOGRAPHIC DISTRIBUTION; HORSE; HUMAN; MALE; MICROBIOLOGICAL EXAMINATION; MICROSCOPY; MOLECULAR DYNAMICS; MYCOSIS; MYIASIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PYTHIUM; PYTHIUM INSIDIOSUM; SPORANGIOSPORE; ZOOSPORE; ZYGOMYCETES;

ANIMALS; BRAZIL; DNA, ALGAL; DNA, RIBOSOMAL SPACER; HORSE DISEASES; HORSES; HUMANS; INFECTION; MALE; MOLECULAR SEQUENCE DATA; PHYLOGENY; PYTHIUM; RNA, RIBOSOMAL, 5.8S; SPORES;

ANIMALIA; EQUIDAE; POACEAE; PYTHIUM; PYTHIUM INSIDIOSUM; ZYGOMYCETES;

EID: 51649127803     PISSN: 13693786     EISSN: 14602709     Source Type: Journal    
DOI: 10.1080/13693780801975014     Document Type: Article

References (41)

1. Triscott, JA, Weedon, D. and Cabana, E. (1993) Human subcutaneous pythiosis. J Cutan Pathol, 20, pp. 267-271.
2. Chaffin, MK, Chumacher, J. and McMullan, WC. (1995) Cutaneus pythiosis in horse. Vet Clin North Am Equine Pract, 11, pp. 91-103.
3. (1985) The Fifth Kingdom, Mycologue Publications, Waterloo, Ontario, Canada
4. Mendoza, L. and Ajello, L. (1996) Infections caused by the oomycetous pathogen Pythium insidiosum. J Mycol Med, 6, pp. 151-164.
5. Kaufman, L. (1998) Penicilliosis marneffei and pythiosis: Emerging tropical diseases. Mycopathologia, 143, pp. 3-7.
6. DeCock, AWAM, Mendoza, L., Padhye, AA, Ajello, L. and Kaufman, L. (1987) Pythium insidiosum sp. nov., the etiologic agent of pythiosis. J Clin Microbiol, 25, pp. 344-349.
7. Dykstra, MJ, Sharp, NJH Olivry, T. et al. (1999) A description of cutaneous-subcutaneus pythiosis in fifteen dogs. Med Mycol, 37, pp. 427-433.
8. Fischer, JR, Pace, LW Turk, JR et al. (1994) Gastrointestinal pythiosis in Missouri dogs: Eleven cases. J Vet Diag Invest, 6, pp. 380-382.
9. Foil, CSO, Short, BG, Fadok, VA and Kunkle, GA. A report of subcutaneous pythiosis in five dogs and a review of the etiologic agent Pythium spp. J Am Animal Hosp Assoc, 20, pp. 959-966.
10. Bissonnette, KW, Sharp, NJH Dykstra, MH et al. (1991) Nasal and retrobulbar mass in a cat caused by Pythium insidiosum. J Med Vet Mycol, 29, pp. 39-44.
11. Miller, RI, Olcott, BM and Archer, M. (1985) Cutaneus pythiosis in beef calves. J Am Vet Med, 186, pp. 983-986.
12. Santurio, JM, Monteiro, AB Leal, AT et al. (1998) Cutaneus Pythiosis insidiosii in calves from the Pantanal region of Brazil. Mycopathologia, 141, pp. 123-125.
13. Perez, RC, Luis-Leon, JJ, Vivas, JL and Mendoza, L. (2005) Epizootic cultaneus pythiosis in beef calves. Vet Microbiol, 109, pp. 121-128.
14. Tabosa, IM, Riet-Correa, F. Nobre, VTM et al. (2004) Outbreaks of pythiosis in two flocks of sheep in northeastern Brazil. Vet Pathol, 41, pp. 412-415.
15. Mendoza, L. and Alfaro, AA. (1986) Equine pythiosis in Costa Rica: Report of 39 cases. Mycopathologia, 94, pp. 123-129.
16. Miller, RI, Shipton, WA and Lea, IR. (1982) Cell wall, zoospores and morphological characteristics of Australian isolates of Pythium causing equine phycomicosis. Trans Br Mycol Soc, 79, pp. 15-23.
17. Miller, RI and Campebell, RSF. (1983) Experimental pythiosis in rabbits. Sabouraudia, 21, pp. 331-341.
18. Leal, ABM, Leal, AT, Santurio, JM, Kommers, GD and Catto, JB. (2001) Pitiose equina no Pantanal brasileiro: Aspectos clinico-patologicos de casos tipico e atipicos. Pesq Vet Bras, 21, pp. 151-156.
19. Luvizari, FH, Lehmkuhl, RC and Santos, IW. (2002) Pitiose equina no Estado do Parana-Primeiro relato de caso. Arch Vet Science, 7, pp. 99-102.
20. Reis Jr, JL and Nogueira, RHG. (2002) Estudo anatomopatologico e imunoistoquimco da pitiose em equinos naturalmente infectados. Arq Bras Med Vet Zootec, 54
21. Reis, JL, de Carvalho, EC, Nogueira, RH, Lemos, LS and Mendoza, L. (2003) Disseminated pythiosis in three horses. Vet Microbiol, 96, pp. 289-295.
22. Sallis, ESV, Pereira, DI and Raffi, MB. (2003) Pitiose cutanea em equinos: 14 casos. Ciencia Rural, 33, pp. 899-903.
23. Headley, AS and Arruda Jr, HN (2004) Equine cutaneous pythiosis: A report of four cases. Ciencia Rural, 34, pp. 289-292.
24. Santurio, JM, Catto, JB, Comastri Filho, JA, Leal, AT and Leal, ABM. (2004) Ferida da Moda. Comunicado Tecnico da Embrapa, 34
25. Bosco, SMG, Bagagli, E. Araujo Jr, JP et al. (2005) Human pythiosis, Brazil. Emerg Infect Dis, 11, pp. 715-717.
26. Marques, SA, Bagagli, E. Bosco, SMG et al. (2006) Pythium insidiosum: relato do primeiro caso de infeccao humana no Brasil. An Bras Dermatol, 81, pp. 483-485.
27. Ridell, RW. (1950) Permanent stained mycological preparations obtained by slide culture. Mycol, 42, pp. 265-270.
28. Mendoza, L. and Prendas, J. (1988) A method to obtain rapid zoosporogenesis of Pythium insidiosum. Mycopathologa, 104, pp. 59-62.
29. McCoullough, MJ, Disalvo, AF, Clemons, KV, Park, P. and Stevens, DA. (2000) Molecular epidemiology of Blastomyces dermatitidis. Clin Infect Dis, 30, pp. 328-335.
30. White, TJ, Burns, T., Lee, S. and Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide To Methods And Applications, pp. 315-322. Academic Press, San Diego
31. Kimura, M. (1981) Estimation of evolutionary distances between homologous nucleotide sequences. Proc Natl Acad Sci USA, 78, pp. 454-458.
32. Schurcko, A., Mendoza, L., DeCock, AWAM and Klassen, GR. (2003) Evidence for geographic clausters: Molecular genetic differences among strains of Pythium insidiosum from Asia, Australia and Americas are explored. Mycologia, 95, pp. 200-208.
33. Ribes, JA, Vanover-Sams, CL and Baker, DJ. (2000) Zygomycetes in human disease. Clin Microbiol Rev, 13, pp. 236-301.
34. Sigler, L., Maza, LM, Tan, G., Egger, KN and Sherburne, RK. (1995) Diagnostic dificulties caused by a nonclamped Schizophyllum commune isolate in a case of fungus ball of the lung. J Clin Microbiol, 33, pp. 1979-1983.
35. Chaiprasert, A., Samerpitak, K., Wanachiwanavin, W. and Thasnakorn, P. (1990) Induction of zoospore formation in Tai isolates of Pythium insidiosum. Mycoses, 33, pp. 317-323.
36. Mendoza, L., Hernandez, F. and Ajello, L. (1993) Life cycle of thee human and animal oomycete pathogen Pythium insidiosum. J Clin Microbiol, 31, pp. 2967-2973.
37. Tabosa, IM, Medeiros, VT and Dantas, AFM. (1999) Pitiose cutanea em equinos no semi-arido da Paraiba. Arq Bras Med Vet Zootec, 51, pp. 27-30.
38. Thianprasit, M. (1986) Fungal infection in Thailand. J Dermatol, 96, pp. 1343-1345.
39. Schurcko, A., Mendoza, L. Levesque, CA et al. (2003) A molecular phylogeny of Pythium insidiosum. Mycol Res, 107, pp. 537-544.
40. Iwen, PC, Hinrichs, SH and Rupp, ME. (2002) Utilization of the internal transcribed spacer regions as molecular targets to detect and identify human fungal pathogens. Med Mycol, 40, pp. 87-109.
41. Grooters, AM and Gee, MK. (2002) Development of a nested polymerase chain reaction assay for the detection and identification of Pythium insidiosum. J Vet Intern Med, 16, pp. 147-152.