Screening for enzyme activity in turbid suspensions with scattered light

Biotechnology Progress

Volumn 27, Issue 2, 2011, Pages 555-561

  Huber, Robert ^a   Wulfhorst, Helene ^a   Maksym, Lukas ^b   Stehr, Regina ^b   Pöhnlein, Martin ^a   Jäger, Gernot ^a   Spieß, Antje C ^a   Büchs, Jochen ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b HENKEL KGAA   (Germany)

Author keywords

High throughput; Microtiter plate; Protease activity; Protein hydrolysis; Turbid suspension

Indexed keywords

HIGH THROUGHPUT; MICROTITER PLATE; PROTEASE ACTIVITIES; PROTEIN HYDROLYSIS; TURBID SUSPENSIONS;

AMINO ACIDS; ENZYMES; HYDROLASES; HYDROLYSIS; LIGHT SCATTERING; SUBSTRATES; SUSPENSIONS (FLUIDS); THROUGHPUT; TURBIDITY;

ENZYME ACTIVITY;

ENZYME; PEPTIDE FRAGMENT; PEPTIDE HYDROLASE; PROTEIN; SERINE PROTEINASE;

ARTICLE; CHEMISTRY; KINETICS; LIGHT; METABOLISM; METHODOLOGY; PHOTOMETRY; RADIATION SCATTERING; SOLUBILITY; SUSPENSION;

ENZYMES; KINETICS; LIGHT; NEPHELOMETRY AND TURBIDIMETRY; PEPTIDE FRAGMENTS; PEPTIDE HYDROLASES; PROTEINS; SCATTERING, RADIATION; SERINE PROTEASES; SOLUBILITY; SUSPENSIONS;

EID: 79954414763     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.519     Document Type: Article

References (23)

1. Arnold FH. Combinatorial and computational challenges for biocatalyst design. Nature. 2001; 409: 253-257.
2. Woodley JM. New opportunities for biocatalysis: making pharmaceutical processes greener. Trends Biotechnol. 2008; 26: 321-327.
3. Maurer KH, Gabler M. Analysis of Detergent Enzymes. New York: Marcel Dekker; 2005.
4. Dunn E, Brothert R. Use of NN-dimethylcasein in determination of proteolytic enzymes in washing products and airborne dust samples. Analyst. 1971; 96: 159-163.
5. Reymond IL. Enzyme Assays, High-Throughput Screening, Genetic Selection and Fingerprinting. Weinheim: Wiley-VCH; 2006.
6. Chen CS, Penner MH. Turbidity-based assay for polygalacturonic acid depolymerase activity. J Agric Food Chem. 2007; 55: 5907-5911.
7. Wang P, Broda P. Stable defined substrate for turbidimetric assay of endoxylanases. Appl Environ Microbiol. 1992; 58: 3433-3436.
8. Fan LT, Lee YH, Beardmore DH. Mechanism of the enzymatic-hydrolysis of cellulose-effects of major structural features of cellulose on enzymatic-Hydrolysis. Biotechnol Bioeng. 1980; 22: 177-199.
9. Gama FM, Carvalho MG, Figueiredo MM, Mota M. Comparative study of cellulose fragmentation by enzymes and ultrasound. Enzyme Microb Technol. 1997; 20: 12-17.
10. Walker LP, Wilson DB, Irwin DC. Measuring fragmentation of cellulose by Thermomonospora fusca cellulase. Enzyme Microb Technol. 1990; 12: 378-386.
11. Bowen P. Particle size distribution measurement from millimeters to nanometers and from rods to platelets. J Dispersion Sci Technol. 2002; 23(5).
12. Virolle MJ, Morris VJ, Bibb MJ. A simple and reliable turbidimetric and kinetic assay for alpha-amylase that is readily applied to culture supernatants and cell-extracts. J Ind Microbiol. 1990; 5: 295-301.
13. Nummi M, Fox PC, Nikupaavola ML, Enari TM. Nephelometric and turbidometric assays of cellulase activity. Anal Biochem. 1981; 116: 133-136.
14. Enari TM, Nikupaavola ML. Nephelometric and turbidometric assay for cellulase. Methods Enzymol. 1988; 160: 117-126.
15. Samorski M, Muller-Newen G, Büchs J. Quasi-continuous combined scattered light and fluorescence measurements: a novel measurement technique for shaken microtiter plates. Biotechnol Bioeng. 2005; 92: 61-68.
16. Kensy F, Zang E, Faulhammer C, Tan RK, Büchs J. Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates. Microb Cell Fact. 2009; 8: 31.
17. Zavrel M, Bross D, Funke M, Büchs J, Spiess AC. High-throughput screening for ionic liquids dissolving (ligno-)cellulose. Bioresour Technol. 2009; 100: 2580-2587.
18. Verbeek H, van Raay HG, Saran H. Zur Bestimmung der proteolytischen Aktivität in Enzymkonzentraten und enzymhaltigen Wasch-, Spül-und Reinigungsmitteln. Tenside Detergens. 1970; 7: 125-132.
19. Latimer P. Light-scattering and absorption as methods of studying cell-population parameters. Ann Rev Biophys Bioeng. 1982; 11: 129-150.
20. Kato Y, Hiraoka S, Tada Y, Nomura T. Performance of a shaking vessel with current pole. Biochem Eng J. 2001; 7: 143-151.
21. Hermann R, Lehmann M, Büchs J. Characterization of gas-liquid mass transfer phenomena in microtiter plates. Biotechnol Bioeng. 2003; 81: 178-186.
22. Bisswanger H. Theorie und Methoden der Enzymkinetik. Aachen: Chemie; 1979.
23. Ghose TK. Measurement of cellulase activities. Pure Appl Chem. 1987; 59: 257-268.