A comparison of methods for extracting DNA from Coxiella burnetii as measured by a duplex qPCR assay

Letters in Applied Microbiology

Volumn 52, Issue 5, 2011, Pages 514-520

  Lockhart, M G ^a,b   Graves, S R ^a   Banazis, M J ^b   Fenwick, S G ^b   Stenos, J ^a  

^a GEELONG HOSPITAL   (Australia)

^b MURDOCH UNIVERSITY   (Australia)

Author keywords

Blood; Bone marrow; Coxiella burnetii; DNA extraction; QPCR

Indexed keywords

BONE; DNA; EXTRACTION; GENES; POLYMERASE CHAIN REACTION; PRECIPITATION (CHEMICAL); SILICA;

BLOOD; BONE MARROW; CLINICAL SAMPLES; COLUMN METHODS; COXIELLUM BURNETII; DNA EXTRACTION; EXTRACTION METHOD; QPCR; SILICA COLUMN; SMALL CELLS;

CHLORINE COMPOUNDS;

BACTERIAL DNA;

ASSAY; BACTERIUM; BLOOD; CELL ORGANELLE; CHLOROFORM; COMPARATIVE STUDY; DNA; GENETIC ANALYSIS; PHENOL; POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SILICA;

ARTICLE; BACTERIUM DETECTION; COXIELLA BURNETII; DNA DETERMINATION; DNA EXTRACTION; INTERMETHOD COMPARISON; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION;

COXIELLA BURNETII; STRAWBERRY CRINKLE VIRUS;

EID: 79953735804     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2011.03034.x     Document Type: Article

References (21)

1. Akane, A., Matsubara, K., Nakamura, H., Takahashi, S. and Kimura, K. (1994) Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification. J Forensic Sci 39, 326-327.
2. Al-Soud, W.A. and Radstrom, P. (2001) Purification and characterization of PCR-inhibitory components in blood cells. J Clin Microbiol 39, 485-493.
3. Al-Soud, W.A., Jonsson, L.J. and Radstrom, P. (2000) Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR. J Clin Microbiol 38, 345-350.
4. Baca, O.G., Akporiaye, E.T., Aragon, A.S., Martinez, I.L., Robles, M.V. and Warner, N.L. (1981) Fate of phase I and phase II Coxiella burnetii in several macrophage-like tumor cell lines. Infect Immun 33, 258-266.
5. Banazis, M.J., Bestall, A.S., Reid, S.A. and Fenwick, S.G. (2010) A survey of Western Australian sheep, cattle and kangaroos to determine the prevalence of Coxiella burnetii. Vet Microbiol 143, 337-345.
6. Clements, D.N., Wood, S., Cater, S.D. and Ollier, W.E. (2008) Assessment of the quality and quantity of genomic DNA recovered from canine blood samples by three different extraction methods. Res Vet Sci 85, 74-79.
7. Coleman, S.A., Fischer, E.R., Howe, D., Mead, D.J. and Heinzen, R.A. (2004) Temporal analysis of Coxiella burnetii morphological differentiation. J Bacteriol 186, 7344-7352.
8. Field, P.R., Mitchell, J.L., Santiago, A., Dickeson, D.J., Chan, S.W., Ho, D.W., Murphy, A.M., Cuzzubbo, A.J. et al. (2000) Comparison of a commercial enzyme-linked immunosorbent assay with immunofluorescence and complement fixation tests for detection of Coxiella burnetii (Q fever) immunoglobulin M. J Clin Microbiol 38, 1645-1647.
9. Fournier, P.E. and Raoult, D. (2003) Comparison of PCR and serology assays for early diagnosis of acute Q fever. J Clin Microbiol 41, 5094-5098.
10. Harris, R.J., Storm, P.A., Lloyd, A., Arens, M. and Marmion, B.P. (2000) Long-term persistence of Coxiella burnetii in the host after primary Q fever. Epidemiol Infect 124, 543-549.
11. Klee, S.R., Tyczka, J., Ellerbrok, H., Franz, T., Linke, S., Baljer, G. and Appel, B. (2006) Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii. BMC Microbiol 6, 2.
12. Kok, T., Wati, S., Bayly, B., Devonshire-Gill, D. and Higgins, G. (2000) Comparison of six nucleic acid extraction methods for detection of viral DNA or RNA sequences in four different specimen types. J Clin Virol 16, 59-63.
13. Marmion, B.P., Storm, P.A., Ayres, J.G., Semendric, L., Mathews, L., Winslow, W., Turra, M. and Harris, R.J. (2005) Long-term persistence of Coxiella burnetii after acute primary Q fever. QJM 98, 7-20.
14. McCaul, T.F. and Williams, J.C. (1981) Developmental cycle of Coxiella burnetii: structure and morphogenesis of vegetative and sporeogenic differentiations. J Bacteriol 147, 1063-1076.
15. Musso, D. and Raoult, D. (1995) Coxiella burnetii blood cultures from acute and chronic Q-fever patients. J Clin Microbiol 33, 3129-3132.
16. Peacock, M.G., Philip, R.N., Williams, J.C. and Faulkner, R.S. (1983) Serological evaluation of Q fever in humans: enhanced phase I titres of immunoglobulins G and A are diagnostic for Q fever endocarditis. Infect Immun 41, 1089-1098.
17. Roussel, Y., Wilks, M., Harris, A., Mein, C. and Tabaqchali, S. (2005) Evaluation of DNA extraction methods from mouse stomachs for the quantification of H. pylori by real-time PCR. J Microbiol Methods 62, 71-81.
18. Self, J.S., Lambourn, O.M., Baszler, T., Raverty, S., Adem, P., Metcalfe, M.G., Zaki, S.R., Kersh, G.J. et al. (2009) Abstract Number 36: placental infection of marine mammals by two novel strains of Coxiella burnetii. Poster presented at the 23rd American Society for Rickettsiology, South Carolina, USA.
19. Wang, J.T., Wang, T.H., Sheu, J.C., Lin, S.M. and Chen, D.S. (1992) Effects of anticoagulants and storage of blood samples on efficacy of the polymerase chain reaction assay for hepatitis C virus. J Clin Microbiol 30, 750-753.
20. Yokota, M., Tatsumi, N., Nathalang, O., Yamada, T. and Tsuda, I. (1999) Effects of heparin on polymerase chain reaction for white blood cells. J Clin Lab Anal 13, 133-140.
21. Zhang, G.Q., To, H., Yamaguchi, T., Fukushi, H. and Hirai, K. (1997) Differentiation of Coxiella burnetii by sequence analysis of the gene (com1) encoding a 27-kDa outer membrane protein. Microbiol Immunol 41, 871-877.