Generating 3D tissue constructs with mesenchymal stem cells and a cancellous bone graft for orthopaedic applications

Biomedical Materials

Volumn 6, Issue 2, 2011, Pages

  Arca, Turkan ^a   Proffitt, Joanne ^b   Genever, Paul ^a  

^a UNIVERSITY OF YORK   (United Kingdom)

^b TSL Centre of Biologics   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BONE; CONFOCAL MICROSCOPY; FLOWCHARTING; PHOSPHATASES; POPULATION STATISTICS; SCAFFOLDS (BIOLOGY); SCANNING ELECTRON MICROSCOPY; STEM CELLS; THREE DIMENSIONAL; TISSUE CULTURE;

ALKALINE PHOSPHATASE ACTIVITY; BONE MATRIX; BONE REGENERATION; CANCELLOUS BONE; COLLAGEN TYPE I; CONFOCAL IMAGING; CROSSLINKED; DERIVED MATERIALS; ENHANCED GREEN FLUORESCENT PROTEIN; GROWTH ANALYSIS; IN-VIVO; LASER SCANNING CONFOCAL MICROSCOPY; MESENCHYMAL STEM CELL; MRNA EXPRESSION LEVEL; ORTHOPAEDIC APPLICATIONS; ORTHOPAEDIC SURGERY; OSTEOCALCIN; OSTEOGENESIS; OSTEOGENIC; OSTEOPONTIN; OSTEOSARCOMA CELLS; TISSUE CONSTRUCTS; TISSUE CULTURE PLASTICS; UP-REGULATION;

CELL CULTURE;

ALKALINE PHOSPHATASE; COLLAGEN TYPE 1; ENHANCED GREEN FLUORESCENT PROTEIN; MESSENGER RNA; OSTEOCALCIN; OSTEONECTIN; OSTEOPONTIN; TRANSCRIPTION FACTOR RUNX2; CROSS LINKING REAGENT; GREEN FLUORESCENT PROTEIN;

ARTICLE; BONE DEVELOPMENT; BONE GRAFT; BONE MATRIX; BONE REGENERATION; CANCELLOUS BONE; CANCER CELL CULTURE; CELL DIFFERENTIATION; CELL GROWTH; CELL INTERACTION; CELL POPULATION; CELL VIABILITY; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; ENZYME ACTIVITY; GENE EXPRESSION; HISTOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMPLANT; MESENCHYMAL STEM CELL; MULTIPOTENT STEM CELL; ORTHOPEDIC SURGERY; OSTEOSARCOMA CELL; PROTEIN EXPRESSION; SCANNING ELECTRON MICROSCOPY; TISSUE ENGINEERING; UPREGULATION; ANIMAL; BONE; BONE MARROW CELL; BONE TRANSPLANTATION; CELL CULTURE; CONFOCAL MICROSCOPY; CYTOLOGY; METABOLISM; METHODOLOGY; ORTHOPEDICS; OSTEOSARCOMA; SWINE; TUMOR CELL LINE;

SUS;

ANIMALS; BONE AND BONES; BONE MARROW CELLS; BONE TRANSPLANTATION; CELL DIFFERENTIATION; CELL LINE, TUMOR; CELLS, CULTURED; CROSS-LINKING REAGENTS; GREEN FLUORESCENT PROTEINS; MESENCHYMAL STEM CELLS; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON, SCANNING; ORTHOPEDICS; OSTEOSARCOMA; SWINE;

EID: 79953726299     PISSN: 17486041     EISSN: 1748605X     Source Type: Journal    
DOI: 10.1088/1748-6041/6/2/025006     Document Type: Article

References (17)

1. Bjerre L, Bunger C E, Kassem M and Mygind T 2008 Flow perfusion culture of human mesenchymal stem cells on silicate-substituted tricalcium phosphate scaffolds Biomaterials 29 2616-27
2. Cornell C N 2004 Osteobiologics Bull. Hosp. Jt. Dis. 62 13-7
3. Dalby M J, McCloy D, Robertson M, Agheli H, Sutherland D, Affrossman S and Oreffo R O 2006a Osteoprogenitor response to semi-ordered and random nanotopographies Biomaterials 27 2980-7
4. Dalby M J, McCloy D, Robertson M, Wilkinson C D and Oreffo R O 2006b Osteoprogenitor response to defined topographies with nanoscale depths Biomaterials 27 1306-15
5. Dyson J A, Genever P G, Dalgarno K W and Wood D J 2007 Development of custom-built bone scaffolds using mesenchymal stem cells and apatite-wollastonite glass-ceramics Tissue Eng. 13 2891-901
6. Etheridge S L, Spencer G J, Heath D J and Genever P G 2004 Expression profiling and functional analysis of Wnt signaling mechanisms in mesenchymal stem cells Stem Cells 22 849-60
7. Giannoudis P V, Dinopoulos H and Tsiridis E 2005 Bone substitutes: an update Injury 36 (Suppl. 3) S20-7
8. Hofmann S, Hagenmuller H, Koch A M, Muller R, Vunjak-Novakovic G, Kaplan D L, Merkle H P and Meinel L 2007 Control of in vitro tissue-engineered bone-like structures using human mesenchymal stem cells and porous silk scaffolds Biomaterials 28 1152-62
9. Kraus K H and Kirker-Head C 2006 Mesenchymal stem cells and bone regeneration Vet. Surg. 35 232-42
10. Logeart-Avramoglou D, Anagnostou F, Bizios R and Petite H 2005 Engineering bone: challenges and obstacles J. Cell Mol. Med. 9 72-84
11. Mauney J R, Blumberg J, Pirun M, Volloch V, Vunjak-Novakovic G and Kaplan D L 2004 Osteogenic differentiation of human bone marrow stromal cells on partially demineralized bone scaffolds in vitro Tissue Eng. 10 81-92
12. Mayr J M, Pierer G R and Linhart W E 2000 Reconstruction of part of the distal tibial growth plate with an autologous graft from the iliac crest J. Bone Joint Surg. Br. 82 558-60
13. Meinel L, Hofmann S, Betz O, Fajardo R, Merkle H P, Langer R, Evans C H, Vunjak-Novakovic G and Kaplan D L 2006 Osteogenesis by human mesenchymal stem cells cultured on silk biomaterials: comparison of adenovirus mediated gene transfer and protein delivery of BMP-2 Biomaterials 27 4993-5002
14. Mizuno M, Fujisawa R and Kuboki Y 2000 Type I collagen-induced osteoblastic differentiation of bone-marrow cells mediated by collagen-alpha2beta1 integrin interaction J. Cell Physiol. 184 207-13 (Pubitemid 30432312)
15. Murphy C M, Haugh M G and O'Brien F J 2010 The effect of mean pore size on cell attachment, proliferation and migration in collagen-glycosaminoglycan scaffolds for bone tissue engineering Biomaterials 31 461-6
16. Mygind T, Stiehler M, Baatrup A, Li H, Zou X, Flyvbjerg A, Kassem M and Bunger C 2007 Mesenchymal stem cell ingrowth and differentiation on coralline hydroxyapatite scaffolds Biomaterials 28 1036-47 (Pubitemid 44809415)
17. Vaccaro A R, Chiba K, Heller J G, Patel T C, Thalgott J S, Truumees E, Fischgrund J S, Craig M R, Berta S C and Wang J C 2002 Bone grafting alternatives in spinal surgery Spine J. 2 206-15