Cell free expression put on the spot: Advances in repeatable protein arraying from DNA (DAPA)

New Biotechnology

Volumn 28, Issue 3, 2011, Pages 282-290

  Stoevesandt, Oda ^a   Vetter, Maike ^b   Kastelic, Damjana ^a,c   Palmer, Elizabeth A ^a   He, Mingyue ^d   Taussig, Michael J ^a,e  

^a Babraham Bioscience Technologies Ltd   (United Kingdom)

^b UNIVERSITY OF KASSEL   (Germany)

^c UNIVERSITY OF LJUBLJANA   (Slovenia)

^d BABRAHAM INSTITUTE   (United Kingdom)

^e Cambridge Protein Arrays Ltd   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARRAY SURFACE; CELL-FREE; CELL-FREE EXPRESSION; CELL-FREE SYSTEM; DIVERSE PROTEINS; DNA ARRAY; IN-SITU; PROTEIN ARRAYS;

DNA; NUCLEIC ACIDS;

PROTEINS;

BETA ARRESTIN 1; CYCLIN DEPENDENT KINASE INHIBITOR 1B; DNA; ENDOGLIN; FORKHEAD TRANSCRIPTION FACTOR; GROWTH FACTOR RECEPTOR BOUND PROTEIN 10; GROWTH FACTOR RECEPTOR BOUND PROTEIN 2; GUANYLATE KINASE; HEAT SHOCK PROTEIN 20; HEME OXYGENASE; INOSITOL POLYPHOSPHATE; INTERLEUKIN 1BETA; K RAS PROTEIN; LECTIN; NOTCH1 RECEPTOR; NUCLEOSIDE PHOSPHORYLASE; PEROXIREDOXIN 1; PHOSPHOGLYCERATE KINASE; RAF PROTEIN; RAS ASSOCIATION DOMAIN FAMILY PROTEIN 1A; REPLICATION FACTOR A; RETINOIC ACID BINDING PROTEIN; SMAD2 PROTEIN; SMAD3 PROTEIN; SMAD7 PROTEIN; THYROID HORMONE RECEPTOR; TISSUE INHIBITOR OF METALLOPROTEINASE 1; TRANSCRIPTION FACTOR; TUMOR NECROSIS FACTOR; UNINDEXED DRUG;

ARTICLE; CELL FREE SYSTEM; CELL LYSATE; CONTROLLED STUDY; DNA MICROARRAY; DNA TEMPLATE; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN MICROARRAY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELL-FREE SYSTEM; DNA; HUMANS; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PROTEIN ARRAY ANALYSIS; PROTEINS;

EID: 79953701026     PISSN: 18716784     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.nbt.2010.09.004     Document Type: Article

References (18)

1. Stoevesandt O., et al. Protein microarrays: high-throughput tools for proteomics. Expert Rev. Proteomics 2009, 6:145-157.
2. Hall D.A., et al. Protein microarray technology. Mech. Ageing Dev. 2007, 128:161-167.
3. Bertone P., Snyder M. Advances in functional protein microarray technology. FEBS J. 2005, 272:5400-5411.
4. Merkel J.S., et al. Functional protein microarrays: just how functional are they?. Curr. Opin. Biotechnol. 2005, 16:447-452.
5. He M., et al. In situ synthesis of protein arrays. Curr. Opin. Biotechnol. 2008, 19:4-9.
6. He M., et al. Printing protein arrays from DNA arrays. Nat. Methods 2008, 5:175-177.
7. Stoevesandt, O. et al. Protein microarrays printed from DNA microarrays. In Biological Microarrays: Methods and Protocols, Methods Mol. Biol. (Vol. 671, Ch. 4) (Khademhosseine, A.; Suh, K.-Y.; Zourob, M. Eds.) Humana Press, 1st Edition., 2010, 304 p. 72 illus., ISBN: 978-1-934115-95-4.
8. Khan F., et al. A double-His tag with high affinity binding for protein immobilisation, purification, and detection on Ni-NTA surfaces. Anal. Chem. 2006, 78:3072-3079.
9. Steinhauer C., et al. Improved affinity coupling for antibody microarrays: engineering of double-(His)6-tagged single framework recombinant antibody fragments. Proteomics 2006, 6:4227-4234.
10. Hartmann M., et al. Increasing robustness and sensitivity of protein microarrays through microagitation and automation. Anal. Chim. Acta 2006, 564:66-73.
11. Goshima N., et al. Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nat. Methods 2008, 5:1011-1017.
12. Ramachandran N., et al. Next-generation high density self-assembling functional protein arrays. Nat. Methods 2008, 5:535-538.
13. Gerber D., et al. An in vitro microfluidic approach to generating protein-interaction networks. Nat. Methods 2009, 6:71-74.
14. He M., Taussig M.J. Single step generation of protein arrays from DNA by cell-free expression and in situ immobilisation (PISA method). Nucleic Acids Res. 2001, 29:E73-E83.
15. He M., Taussig M.J. DiscernArray technology: a cell-free method for the generation of protein arrays from PCR DNA. J. Immunol. Methods 2003, 274:265-270.
16. Rajesh, S. et al. (2010) Production of membrane proteins without cells or detergents. Nat. Biotechnol. [Epub ahead of print], this issue.
17. Junge, F. et al. (2010) Advances in cell-free protein synthesis for the functional and structural analysis of membrane proteins. Nat. Biotechnol. [Epub ahead of print], this issue.
18. Ptacek J., et al. Global analysis of protein phosphorylation in yeast. Nature 2005, 438:679.