Purification and identification of O-GlcNAc-modified peptides using phosphate-based alkyne CLICK chemistry in combination with titanium dioxide chromatography and mass spectrometry

Journal of Proteome Research

Volumn 10, Issue 4, 2011, Pages 1449-1458

  Parker, Benjamin L ^a,b   Gupta, Pankaj ^c   Cordwell, Stuart J ^b   Larsen, Martin R ^a   Palmisano, Giuseppe ^a  

^a UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^b UNIVERSITY OF SYDNEY   (Australia)

^c BINGHAMTON UNIVERSITY   (United States)

Author keywords

chemoenzymatic labeling; ETD MS MS; HCD MS MS; O GlcNAc; phosphorylation; post translational modifications; titanium dioxide chromatography

Indexed keywords

ALKYNE; ALPHA CRYSTALLIN; N ACETYLGLUCOSAMINE; NUCLEAR PROTEIN; O LINKED BETA N ACETYLGLUCOSAMINE; PEPTIDE; PHOSPHATE; TITANIUM DIOXIDE; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; BRAIN TISSUE; CHROMATOGRAPHY; ELECTRON TRANSPORT; HELA CELL; HUMAN; HUMAN CELL; MASS SPECTROMETRY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; PROTEIN PURIFICATION;

ACETYLGLUCOSAMINE; ALKYNES; ALPHA-CRYSTALLINS; AMINO ACID SEQUENCE; ANIMALS; CATTLE; CHROMATOGRAPHY; GLYCOSYLATION; HELA CELLS; HUMANS; MASS SPECTROMETRY; MICE; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; NUCLEAR PROTEINS; PEPTIDES; PROTEOMICS; TITANIUM;

EID: 79953679905     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr100565j     Document Type: Article

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