Chitin-based materials in tissue engineering: Applications in soft tissue and epithelial organ

International Journal of Molecular Sciences

Volumn 12, Issue 3, 2011, Pages 1936-1963

  Yang, Tsung Lin ^a,b  

^a NATIONAL TAIWAN UNIVERSITY HOSPITAL   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

Chitin; Chitosan; Epithelium; Regeneration; Soft tissue; Tissue engineering

Indexed keywords

BIOMATERIAL; CHITIN; TISSUE SCAFFOLD; CHITOSAN;

ADIPOSE TISSUE; CELL INFILTRATION; CELL SURVIVAL; CELL VIABILITY; CONNECTIVE TISSUE; DRUG DELIVERY SYSTEM; EXTRACELLULAR MATRIX; GENE EXPRESSION REGULATION; HUMAN; MOLECULAR WEIGHT; NERVOUS TISSUE; NONHUMAN; PARTICLE SIZE; PHENOTYPE; REVIEW; SOFT TISSUE; TISSUE ADHESION; TISSUE ENGINEERING; TISSUE REGENERATION; TISSUE STRUCTURE; VASCULAR TISSUE; CHEMISTRY; DRUG EFFECTS; EPITHELIUM; METABOLISM; PHYSIOLOGY; REGENERATION; TISSUE SCAFFOLD; WOUND HEALING;

BIOCOMPATIBLE MATERIALS; CHITIN; CHITOSAN; EPITHELIUM; EXTRACELLULAR MATRIX; REGENERATION; TISSUE ENGINEERING; TISSUE SCAFFOLDS; WOUND HEALING;

EID: 79953321657     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms12031936     Document Type: Review

References (150)

1. Kumar, M.N.; Muzzarelli, R.A.; Muzzarelli, C.; Sashiwa, H.; Domb, A.J. Chitosan chemistry and pharmaceutical perspectives. Chem. Rev. 2004, 104, 6017-6084.
2. Felt, O.; Furrer, P.; Mayer, J.M.; Plazonnet, B.; Buri, P.; Gurny, R. Topical use of chitosan in ophtalmology: Tolerance assessment and evaluation of precorneal retention. Int. J. Pharm. 1999, 180, 185-193.
3. Muzzarelli, R.A.A. Human enzymatic activities related to the therapeutic administration of chitin derivatives. Cell. Mol. Life Sci. 1997, 53, 131-140.
4. Kim, I.Y.; Seo, S.J.; Moon, H.S.; Yoo, M.K.; Park, I.Y.; Kim, B.C.; Cho, C.S. Chitosan and its derivatives for tissue engineering applications. Biotechnol. Adv. 2008, 26, 1-21.
5. Chung, Y.C.; Chen, C.Y. Antibacterial characteristics and activity of acid-soluble chitosan. Bioresour. Technol. 2008, 99, 2806-2814.
6. He, P.; Davis, S.S.; Illum, L. In vitro evaluation of the mucoadhesive properties of chitosan microspheres. Int. J. Pharm. 1998, 166, 75-88.
7. Calvo, P.; Vila-Jato, J.L.; Alonso, M.J. Evaluation of cationic polymer-coated nanocapsules as ocular drug carriers. Int. J. Pharm. 1997, 153, 41-50.
8. Ishihara, M.; Fujita, M.; Obara, K.; Hattori, H.; Nakamura, S.; Nambu, M.; Kiyosawa, T.; Kanatani, Y.; Takase, B.; Kikuchi, M.; Maehara, T. Controlled releases of FGF-2 and paclitaxel from chitosan hydrogels and their subsequent effects on wound repair, angiogenesis, and tumor growth. Curr. Drug. Deliv. 2006, 3, 351-358.
9. Mori, T.; Okumura, M.; Matsuura, M.; Ueno, K.; Tokura, S.; Okamoto, Y.; Minami, S.; Fujinaga, T. Effects of chitin and its derivatives on the proliferation and cytokine production of fibroblasts in vitro. Biomaterials 1997, 18, 947-951.
10. Masuoka, K.; Ishihara, M.; Asazuma, T.; Hattori, H.; Matsui, T.; Takase, B.; Kanatani, Y.; Fujita, M.; Saito, Y.; Yura, H.; Fujikawa, K.; Nemoto, K. The interaction of chitosan with fibroblast growth factor-2 and its protection from inactivation. Biomaterials 2005, 26, 3277-3284.
11. Madihally, S.V.; Matthew, H.W. Porous chitosan scaffolds for tissue engineering. Biomaterials 1999, 20, 1133-1142.
12. Yang, T.L.; Young, T.H. Chitosan cooperates with mesenchyme-derived factors in regulating salivary gland epithelial morphogenesis. J. Cell. Mol. Med. 2009, 13, 2853-2863.
13. Mi, F.L.; Shyu, S.S.; Peng, C.K.; Wu, Y.B.; Sung, H.W.; Wang, P.S.; Huang, C.C. Fabrication of chondroitin sulfate-chitosan composite artificial extracellular matrix for stabilization of fibroblast growth factor. J. Biomed. Mater. Res. A 2006, 76, 1-15.
14. Prabaharan, M. Review paper: Chitosan derivatives as promising materials for controlled drug delivery. J. Biomater. Appl. 2008, 23, 5-36.
15. Jiang, T.; Kumbar, S.G.; Nair, L.S.; Laurencin, C.T. Biologically active chitosan systems for tissue engineering and regenerative medicine. Curr. Top. Med. Chem. 2008, 8, 354-364.
16. Langer, R.; Vacanti, J.P.; Vacanti, C.A.; Atala, A.; Freed, L.E.; Vunjak-Novakovic, G. Tissue engineering: biomedical applications. Tissue Eng. 1995, 1, 151-161.
17. Ladd, M.R.; Lee, S.J.; Atala, A.; Yoo, J.J. Bioreactor maintained living skin matrix. Tissue Eng. Part A 2009, 15, 861-868.
18. Atala, A. Tissue engineering of artificial organs. J. Endourol. 2000, 14, 49-57.
19. Atala, A.; Bauer, S.B.; Soker, S.; Yoo, J.J.; Retik, A.B. Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet 2006, 367, 1241-1246.
20. Macchiarini, P.; Jungebluth, P.; Go, T.; Asnaghi, M.A.; Rees, L.E.; Cogan, T.A.; Dodson, A.; Martorell, J.; Bellini, S.; Parnigotto, P.P.; Dickinson, S.C.; Hollander, A.P.; Mantero, S.; Conconi, M.T.; Birchall, M.A. Clinical transplantation of a tissue-engineered airway. Lancet 2008, 372, 2023-2030.
21. Langer, R.; Vacanti, J.P. Tissue engineering. Science 1993, 260, 920-926.
22. Peter, M.G. Applications and environmental aspects of chitin and chitosan. J. Macromol. Sci. 1995, Part A, 629-640.
23. Berger, J.; Reist, M.; Mayer, J.M.; Felt, O.; Peppas, N.A.; Gurny, R. Structure and interactions in covalently and ionically crosslinked chitosan hydrogels for biomedical applications. Eur. J. Pharm. Biopharm. 2004, 57, 19-34.
24. Hu, Q.; Li, B.; Wang, M.; Shen, J. Preparation and characterization of biodegradable chitosan/hydroxyapatite nanocomposite rods via in situ hybridization: A potential material as internal fixation of bone fracture. Biomaterials 2004, 25, 779-785.
25. Muzzarelli, R.; Baldassarre, V.; Conti, F.; Ferrara, P.; Biagini, G.; Gazzanelli, G.; Vasi, V. Biological activity of chitosan: Ultrastructural study. Biomaterials 1988, 9, 247-252.
26. Khor, E.; Lim, L.Y. Implantable applications of chitin and chitosan. Biomaterials 2003, 24, 2339-2349.
27. Felt, O.; Carrel, A.; Baehni, P.; Buri, P.; Gurny, R. Chitosan as tear substitute: A wetting agent endowed with antimicrobial efficacy. J. Ocul. Pharmacol. 2000, 16, 261-270.
28. Ayres, C.E.; Jha, B.S.; Sell, S.A.; Bowlin, G.L.; Simpson, D.G. Nanotechnology in the design of soft tissue scaffolds: Innovations in structure and function. Wiley Interdiscip. Rev. Nanomed. Nanobiotech. 2010, 2, 20-34.
29. Di Martino, A.; Sittinger, M.; Risbud, M.V. Chitosan: A versatile biopolymer for orthopaedic tissue-engineering. Biomaterials 2005, 26, 5983-5990.
30. Risbud, M.V.; Sittinger, M. Tissue engineering: Advances in in vitro cartilage generation. Trends Biotech. 2002, 20, 351-356.
31. Oliveira, J.M.; Rodrigues, M.T.; Silva, S.S.; Malafaya, P.B.; Gomes, M.E.; Viegas, C.A.; Dias, I.R.; Azevedo, J.T.; Mano, J.F.; Reis, R.L. Novel hydroxyapatite/chitosan bilayered scaffold for osteochondral tissue-engineering applications: Scaffold design and its performance when seeded with goat bone marrow stromal cells. Biomaterials 2006, 27, 6123-6137.
32. Hemmrich, K.; von Heimburg, D.; Rendchen, R.; Di Bartolo, C.; Milella, E.; Pallua, N. Implantation of preadipocyte-loaded hyaluronic acid-based scaffolds into nude mice to evaluate potential for soft tissue engineering. Biomaterials 2005, 26, 7025-7037.
33. Ueno, H.; Yamada, H.; Tanaka, I.; Kaba, N.; Matsuura, M.; Okumura, M.; Kadosawa, T.; Fujinaga, T. Accelerating effects of chitosan for healing at early phase of experimental open wound in dogs. Biomaterials 1999, 20, 1407-1414.
34. Mattioli-Belmonte, M.; Muzzarelli, B.; Muzzarelli, R.A.A. Chitin and chitosan in wound healing and other biomedical applications. Carbohyd. Eur. 1997, 19, 30-36.
35. Cho, Y.W.; Cho, Y.N.; Chung, S.H.; Yoo, G.; Ko, S.W. Water-soluble chitin as a wound healing accelerator. Biomaterials 1999, 20, 2139-2145.
36. Kojima, K.; Okamoto, Y.; Kojima, K.; Miyatake, K.; Fujise, H.; Shigemasa, Y.; Minami, S. Effects of chitin and chitosan on collagen synthesis in wound healing. J. Vet. Med. Sci. 2004, 66, 1595-1598.
37. VandeVord, P.J.; Matthew, H.W.T.; DeSilva, S.P.; Mayton, L.; Wu, B.; Wooley, P.H. Evaluation of the biocompatibility of a chitosan scaffold in mice. J. Biomed. Mater. Res. 2002, 59, 585-590.
38. Drewa, T.; Adamowicz, J.; Lysik, J.; Polaczek, J.; Pielichowski, J. Chitosan scaffold enhances nerve regeneration within the in vitro reconstructed bladder wall: An animal study. Urol. Int. 2008, 81, 330-334.
39. Berger, J.; Reist, M.; Mayer, J.M.; Felt, O.; Gurny, R. Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications. Eur. J. Pharm. Biopharm. 2004, 57, 35-52.
40. Chow, K.S.; Khor, E. Novel fabrication of open-pore chitin matrixes. Biomacromolecules 2000, 1, 61-67.
41. Shi, C.; Zhu, Y.; Ran, X.; Wang, M.; Su, Y, Cheng, T. Therapeutic potential of chitosan and its derivatives in regenerative medicine. J. Surg. Res. 2006, 133, 185-192.
42. Freier, T.; Montenegro, R.; Shan Koh, H.; Shoichet, M.S. Chitin-based tubes for tissue engineering in the nervous system. Biomaterials 2005, 26, 4624-4632.
43. Wang, W.; Itoh, S.; Matsuda, A.; Ichinose, S.; Shinomiya, K.; Hata, Y.; Tanaka, J. Influences of mechanical properties and permeability on chitosan nano/microfiber mesh tubes as a scaffold for nerve regeneration. J. Biomed. Mater. Res. A 2008, 84, 557-566.
44. Zhang, L.; Ao, Q.; Wang, A.; Lu, G.; Kong, L.; Gong, Y.; Zhao, N.; Zhang, X. A sandwich tubular scaffold derived from chitosan for blood vessel tissue engineering. J. Biomed. Mater. Res. A 2006, 77, 277-284.
45. Madihally, S.V.; Matthew, H.W. Porous chitosan scaffolds for tissue engineering. Biomaterials 1999, 20, 1133-1142.
46. Ko, Y.G.; Kawazoe, N.; Tateishi, T.; Chen, G. Preparation of chitosan scaffolds with a hierarchical porous structure. J. Biomed. Mater. Res. B 2010, 93, 341-350.
47. Elsabee, M.Z.; Morsi, R.E.; Al-Sabagh, A.M. Surface active properties of chitosan and its derivatives. Colloids Surf. B 2009, 74, 1-16.
48. Nishikawa, H.; Ueno, A.; Nishikawa, S.; Kido, J.; Ohishi, M.; Inoue, H.; Nagata, T. Sulfated glycosaminoglycan synthesis and its regulation by transforming growth factor-beta in rat clonal dental pulp cells. J. Endod. 2000, 26, 169-171.
49. Bagnaninchi, P.O.; Yang, Y.; Zghoul, N.; Maffulli, N.; Wang, R.K.; Haj, A.J. Chitosan microchannel scaffolds for tendon tissue engineering characterized using optical coherence tomography. Tissue Eng. 2007, 13, 323-331.
50. Ma, J.; Wang, H.;He, B.; Chen, J. A preliminary in vitro study on the fabrication and tissue engineering applications of a novel chitosan bilayer material as a scaffold of human fetal dermal fibroblasts. Biomaterials 2001, 22, 331-336.
51. Lin, S.J.; Jee, S.H.; Hsaio, W.C.; Lee, S.J.; Young, T.H. Formation of melanocyte spheroids on the chitosan-coated surface. Biomaterials 2005, 26, 1413-1422.
52. Norazril, S.A.; Aminuddin, B.S.; Norhayati, M.M.; Mazlyzam, A.L.; Fauziah, O.; Ruszymah, B. H. Comparison of chitosan scaffold and chitosan-collagen scaffold: A preliminary study. Med. J. Malaysia 2004, 59, 186-187.
53. Huang, T.W.; Young, Y.H.; Cheng, P.W.; Chan, Y.H.; Young, T.H. Culture of nasal epithelial cells using chitosan-based membranes. Laryngoscope 2009, 119, 2066-2070.
54. Shao, H.J.; Chen, C.S.; Lee, Y.T.; Wang, J.H.; Young, T.H. The phenotypic responses of human anterior cruciate ligament cells cultured on poly(epsilon-caprolactone) and chitosan. J. Biomed. Mater. Res. A 2010, 93, 1297-1305.
55. Lin, S.J.; Hsiao, W.C.; Jee, S.H.; Yu, H.S.; Tsai, T.F.; Lai, J.Y.; Young, T.H. Study on the effects of nylon-chitosan-blended membranes on the spheroid-forming activity of human melanocytes. Biomaterials 2006, 27, 5079-5088.
56. Crompton, K.E.; Goud, J.D.; Bellamkonda, R.V.; Gengenbach, T.R.; Finkelstein, D.I.; Horne, M.K.; Forsythe, J.S. Polylysine-functionalised thermoresponsive chitosan hydrogel for neural tissue engineering. Biomaterials 2007, 28, 441-449.
57. Funakoshi, T.; Majima, T.; Iwasaki, N.; Yamane, S.; Masuko, T.; Minami, A.; Harada, K.; Tamura, H.; Tokura, S.; Nishimura, S. Novel chitosan-based hyaluronan hybrid polymer fibers as a scaffold in ligament tissue engineering. J. Biomed. Mater. Res. A 2005, 74, 338-346.
58. Zhu, C.; Fan, D.; Duan, Z.; Xue, W.; Shang, L.; Chen, F.; Luo, Y. Initial investigation of novel human-like collagen/chitosan scaffold for vascular tissue engineering. J. Biomed. Mater. Res. A 2009, 89, 829-840.
59. Shi, H.; Han, C.; Mao, Z.; Ma, L.; Gao, C. Enhanced angiogenesis in porous collagen-chitosan scaffolds loaded with angiogenin. Tissue Eng. 2008, 14, 1775-1785.
60. Wu, X.; Black, L.; Santacana-Laffitte, G.; Patrick, C.W., Jr. Preparation and assessment of glutaraldehyde-crosslinked collagen-chitosan hydrogels for adipose tissue engineering. J. Biomed. Mater. Res. A 2007, 81, 59-65.
61. Gobin, A.S.; Froude, V.E.; Mathur, A.B. Structural and mechanical characteristics of silk fibroin and chitosan blend scaffolds for tissue regeneration. J. Biomed. Mater. Res. A 2005, 74, 465-473.
62. Gobin, A.S.; Butler, C.E.; Mathur, A.B. Repair and regeneration of the abdominal wall musculofascial defect using silk fibroin-chitosan blend. Tissue Eng. 2006, 12, 3383-3394.
63. Ma, L.; Gao, C.Y.; Mao, Z.W.; Zhou, J.; Shen, J.C.; Hu, X.Q.; Han, C.M. Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering. Biomaterials 2003, 24, 4833-4841.
64. Han, C.M.; Zhang, L.P.; Sun, J.Z.; Shi, H.F.; Zhou, J.; Gao, C.Y. Application of collagen-chitosan/fibrin glue asymmetric scaffolds in skin tissue engineering. J. Zhejiang Univ. Sci. B 2010, 11, 524-530.
65. Kinikoglu, B.; Auxenfans, C.; Pierrillas, P.; Justin, V.; Breton, P.; Burillon, C.; Hasirci, V.; Damour, O. Reconstruction of a full-thickness collagen-based human oral mucosal equivalent. Biomaterials 2009, 30, 6418-6425.
66. Yang, J.; Woo, S.L.; Yang, G.; Wang, J.; Cui, L.; Liu, W.; Cao, Y. Construction and clinical application of a human tissue-engineered epidermal membrane. Plast Reconstr. Surg. 2010, 125, 901-909.
67. Gafni, Y.; Zilberman, Y.; Ophir, Z.; Abramovitch, R.; Jaffe, M.; Gazit, Z.; Domb, A.; Gazit, D. Design of a filamentous polymeric scaffold for in vivo guided angiogenesis. Tissue Eng. 2006, 12, 3021-3034.
68. Lee, J.J.; Lee, S.; Park, J.C.; Yang, Y.I.; Kim, J.K. Investigation on biodegradable PLGA scaffold with various pore size structure for skin tissue engineering. Curr. Appl. Phys. 2007, 7, e37-e40.
69. Pieper, J.S.; van Wachem, P.B.; van Luyn, M.J.A.; Brouwer, L.A.; Hafmans, T.; Veerkamp, J.H.; van Kuppevelt, T.H. Attachment of glycosaminoglycans to collagenous matrices modulates the tissue response in rats. Biomaterials 2000, 21, 1689-1699.
70. Ragetly, G.; Griffon, D.J.; Chung, Y.S. The effect of type II collagen coating of chitosan fibrous scaffolds on mesenchymal stem cell adhesion and chondrogenesis. Acta Biomater. 2010, 6, 3988-3997.
71. Ragetly, G.R.; Griffon, D.J.; Lee, H.B.; Chung, Y.S. Effect of collagen II coating on mesenchymal stem cell adhesion on chitosan and on reacetylated chitosan fibrous scaffolds. J. Mater. Sci. Mater. Med. 2010, 21, 2479-2490.
72. Leipzig, N.D.; Xu, C.; Zahir, T.; Shoichet, M.S. Functional immobilization of interferon-gamma induces neuronal differentiation of neural stem cells. J. Biomed. Mater. Res. A 2010, 93, 625-633.
73. Akman, A.C.; Tigli, R.S.; Gumusderelioglu, M.; Nohutcu, R.M. bFGF-loaded HA-chitosan: A promising scaffold for periodontal tissue engineering. J. Biomed. Mater. Res. A 2010, 92, 953-962.
74. Tabata, Y. Tissue regeneration based on growth factor release. Tissue Eng. 2003, 9 Suppl 1, s5-15.
75. Berger, J.; Reist, M.; Mayer, J.M.; Felt, O.; Gurny, R. Structure and interactions in chitosan hydrogels formed by complexation or aggregation for biomedical applications. Eur. J. Pharm. Biopharm. 2004, 57, 35-52.
76. Ho, M.H.; Wang, D.M.; Hsieh, H.J.; Liu, H.C.; Hsien, T.Y.; Lai, J.Y.; Hou, L.T. Preparation and characterization of RGD-immobilized chitosan scaffolds. Biomaterials 2005, 26, 3197-3206.
77. Yamada, Y.; Hozumi, K.; Katagiri, F.; Kikkawa, Y.; Nomizu, M. Biological activity of laminin peptide conjugated alginate and chitosan matrices. Biopolymers 2010, 94, 711-720.
78. Kean, T.; Roth, S.; Thanou, M. Trimethylated chitosans as non-viral gene delivery vectors: Cytotoxicity and transfection efficiency. J. Control. Release 2005, 103, 643-653.
79. Itoh, S.; Matsuda, A.; Kobayashi, H.; Ichinose, S.; Shinomiya, K.; Tanaka, J. Effects of a laminin peptide (YIGSR) immobilized on crab-tendon chitosan tubes on nerve regeneration. J. Biomed. Mater. Res. B 2005, 73, 375-382.
80. Mizuno, K.; Yamamura, K.; Yano, K.; Osada, T.; Saeki, S.; Takimoto, N.; Sakurai, T.; Nimura, Y. Effect of chitosan film containing basic fibroblast growth factor on wound healing in genetically diabetic mice. J. Biomed. Mater. Res. A 2003, 64, 177-181.
81. Choi, J.S.; Yoo, H.S. Pluronic/chitosan hydrogels containing epidermal growth factor with wound-adhesive and photo-crosslinkable properties. J. Biomed. Mater. Res. A 2010, 95A, 564-573.
82. Judith, R.; Nithya, M.; Rose, C.; Mandal, A.B. Application of a PDGF-containing novel gel for cutaneous wound healing. Life Sci. 2010, 87, 1-8.
83. Ishihara, M.; Obara, K.; Ishizuka, T.; Fujita, M.; Sato, M.; Masuoka, K.; Saito, Y.; Yura, H.; Matsui, T.; Hattori, H.; Kikuchi, M.; Kurita, A. Controlled release of fibroblast growth factors and heparin from photocrosslinked chitosan hydrogels and subsequent effect on in vivo vascularization. J. Biomed. Mater. Res. A 2003, 64, 551-559.
84. Guo, R.; Xu, S.; Ma, L.; Huang, A.; Gao, C. Enhanced angiogenesis of gene-activated dermal equivalent for treatment of full thickness incisional wounds in a porcine model. Biomaterials 2010, 31, 7308-7320.
85. Zhang, Y.; Cheng, X.; Wang, J.; Wang, Y.; Shi, B.; Huang, C.; Yang, X.; Liu, T. Novel chitosan/collagen scaffold containing transforming growth factor-beta1 DNA for periodontal tissue engineering. Biochem. Biophys. Res. Commun. 2006, 344, 362-369.
86. Lin, S.J.; Jee, S.H.; Hsiao, W.C.; Yu, H.S.; Tsai, T.F.; Chen, J.S.; Hsu, C.J.; Young, T.H. Enhanced cell survival of melanocyte spheroids in serum starvation condition. Biomaterials 2006, 27, 1462-1469.
87. Chen, Y.H.; Wang, I.J.; Young, T.H. Formation of keratocyte spheroids on chitosan-coated surface can maintain keratocyte phenotypes. Tissue Eng. Part A 2009, 15, 2001-2013.
88. Shao, H.J.; Chen, C.S.; Lee, Y.T.; Wang, J.H.; Young, T.H. The phenotypic responses of human anterior cruciate ligament cells cultured on poly(epsilon-caprolactone) and chitosan. J. Biomed. Mater. Res. A 2010, 93, 1297-1305.
89. Wang, G.; Ao, Q.; Gong, K.; Wang, A.; Zheng, L.; Gong, Y.; Zhang, X. The effect of topology of chitosan biomaterials on the differentiation and proliferation of neural stem cells. Acta Biomater. 2010, 6, 3630-3639.
90. Zhu, Y.; Liu, T.; Song, K.; Jiang, B.; Ma, X.; Cui, Z. Collagen-chitosan polymer as a scaffold for the proliferation of human adipose tissue-derived stem cells. J. Mater. Sci. Mater. Med. 2009, 20, 799-808.
91. Thein-Han, W.W.; Kitiyanant, Y. Chitosan scaffolds for in vitro buffalo embryonic stem-like cell culture: an approach to tissue engineering. J. Biomed. Mater. Res. B 2007, 80, 92-101.
92. Gupta, V.; Davis, G.; Gordon, A.; Altman, A.M.; Reece, G.P.; Gascoyne, P.R.; Mathur, A.B. Endothelial and stem cell interactions on dielectrophoretically aligned fibrous silk fibroin-chitosan scaffolds. J. Biomed. Mater. Res. A 2010, 94, 515-523.
93. Lahiji, A.; Sohrabi, A.; Hungerford, D.S.; Frondoza, C.G. Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes. J. Biomed. Mater. Res. 2000, 51, 586-595.
94. Shen, E.C.; Chou, T.C.; Gau, C.H.; Tu, H.P.; Chen, Y.T.; Fu, E. Releasing growth factors from activated human platelets after chitosan stimulation: a possible bio-material for platelet-rich plasma preparation. Clin. Oral Implants. Res. 2006, 17, 572-578.
95. Ueno, H.; Nakamura, F.; Murakami, M.; Okumura, M.; Kadosawa, T.; Fujinag, T. Evaluation effects of chitosan for the extracellular matrix production by fibroblasts and the growth factors production by macrophages. Biomaterials 2001, 22, 2125-2130.
96. Shao, H.J.; Lee, Y.T.; Chen, C.S.; Wang, J.H.; Young, T.H. Modulation of gene expression and collagen production of anterior cruciate ligament cells through cell shape changes on polycaprolactone/chitosan blends. Biomaterials 2010, 31, 4695-4705.
97. Lopes, J.B.; Dallan, L.A.; Moreira, L.F.; Campana Filho, S.P.; Gutierrez, P.S.; Lisboa, L.A.; de Oliveira, S.A.; Stolf, N.A. Synergism between keratinocyte growth factor and carboxymethyl chitosan reduces pericardial adhesions. Ann. Thorac. Surg. 2010, 90, 566-572.
98. Yang, T.L.; Young, T.H. The enhancement of submandibular gland branch formation on chitosan membranes. Biomaterials 2008, 29, 2501-2508.
99. Okamoto, Y.; Shibazaki, K.; Minami, S.; Matsuhashi, A.; Tanioka, S.; Shigemasa, Y. Evaluation of chitin and chitosan on open would healing in dogs. J. Vet. Med. Sci. 1995, 57, 851-854.
100. Kojima, K.; Okamoto, Y.; Miyatake, K.; Fujise, H.; Shigemasa, Y.; Minami, S. Effects of chitin and chitosan on collagen synthesis in wound healing. J. Vet. Med. Sci. 2004, 66, 1595-1598.
101. Kojima, K.; Okamoto, Y.; Miyatake, K.; Kitamura, Y.; Minami, S. Collagen typing of granulation tissue induced by chitin and chitosan. Carbohyd. Polym. 1998, 37, 109-113.
102. Hsiao, Y.C.; Lee, H.W.; Chen, Y.T.; Young, T.H.; Yang, T.L. The impact of compositional topography of amniotic membrane scaffold on tissue morphogenesis of salivary gland. Biomaterials 2011 (in press); doi: 10.1016/j.biomaterials.2011.02.057.
103. Funakoshi, T.; Majima, T.; Iwasaki, N.; Suenaga, N.; Sawaguchi, N.; Shimode, K.; Minami, A.; Harada, K.; Nishimura, S. Application of tissue engineering techniques for rotator cuff regeneration using a chitosan-based hyaluronan hybrid fiber scaffold. Am. J. Sports. Med. 2005, 33, 1193-1201.
104. Chen, W.L.; Huang, C.H.; Chiou, L.L.; Chen, T.H.; Huang, Y.Y.; Jiang, C.C.; Lee, H.S.; Dong, C.Y. Multiphoton imaging and quantitative analysis of collagen production by chondrogenic human mesenchymal stem cells cultured in chitosan scaffold. Tissue Eng. 2010, 16, 913-920.
105. Jayakumar, R.; Prabaharan, M.; Kumar, P.T.; Nair, S.V.; Tamura, H. Biomaterials based on chitin and chitosan in wound dressing applications. Biotechnol. Adv. 2011.
106. Tashiro, T. Antibacterial and bacterium adsorbing macromolecules. Macromol. Mater. Eng. 2001, 286, 63-87.
107. Azad, A.K.; Sermsintham, N.; Chandrkrachang, S.; Stevens, W.F. Chitosan membrane as a wound-healing dressing: Characterization and clinical application. J. Biomed. Mater. Res. B 2004, 69, 216-222.
108. Kozen, B.G.; Kircher, S.J.; Henao, J.; Godinez, F.S.; Johnson, A.S. An alternative hemostatic dressing: comparison of CELOX, HemCon, and QuikClot. Acad. Emerg. Med. 2008, 15, 74-81.
109. Yannas, I.V.; Burke, J.F.; Gordon, P.L.; Huang, C.; Rubenstein, R.H. Design of an artificial skin. II. Control of chemical composition. J. Biomed. Mater. Res. 1980, 14, 107-132.
110. Auxenfans, C.; Fradette, J.; Lequeux, C.; Germain, L.; Kinikoglu, B.; Bechetoille, N.; Braye, F.; Auger, F.A.; Damour, O. Evolution of three dimensional skin equivalent models reconstructed in vitro by tissue engineering. Eur. J. Dermatol. 2009, 19, 107-113.
111. Yannas, I.V.; Burke, J.F. Design of an artificial skin. I. Basic design principles. J. Biomed. Mater. Res. 1980, 14, 65-81.
112. Tsai, R.J.; Li, L.M.; Chen, J.K. Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells. N. Engl. J. Med. 2000, 343, 86-93.
113. Yeh, L.K.; Chen, Y.H.; Chiu, C.S.; Hu, F.R.; Young, T.H.; Wang, I.J. The phenotype of bovine corneal epithelial cells on chitosan membrane. J. Biomed. Mater. Res. A 2009, 90, 18-26.
114. Friedman, M.; Ibrahim, H.; Ramakrishnan, V. Inferior turbinate flap for repair of nasal septal perforation. Laryngoscope 2003, 113, 1425-1428.
115. Davenport, E.A.; Nettesheim, P. Regulation of mucociliary differentiation of rat tracheal epithelial cells by type I collagen gel substratum. Am. J. Respir. Cell Mol. Biol. 1996, 14, 19-26.
116. Nishijkawa, A.; Taira, T.; Yoshizato, K. In vitro maturation of collagen fibrils modulates spreading, DNA synthesis, and collagenolysis of epidermal cells and fibroblast. Exp. Cell Res. 1987, 171, 164-177.
117. Taravel, M.N.; Domard, A. Collagen and its interactions with chitosan, III some biological and mechanical properties. Biomaterials 1996, 17, 451-455.
118. Affolter, M.; Zeller, R.; Caussinus, E. Tissue remodelling through branching morphogenesis. Nat. Rev. Mol. Cell Biol. 2009, 10, 831-842.
119. Affolter, M.; Bellusci, S.; Itoh, N.; Shilo, B.; Thiery, J.P.; Werb, Z. Tube or not tube: Remodeling epithelial tissues by branching morphogenesis. Dev. Cell 2003, 4, 11-18.
120. Yang, T.L.; Hsiao, Y.C.; Young, T.H. Comparison of PLGA, PCL, and chitosan in salivary gland branching morphogenesis. Biomed. Eng.-App. Bas. C 2008, 20, 287-296.
121. Yang, T.L.; Hsiao, Y.C.; Lin, S.J.; Lee, H.W.; Lou, P.J.; Ko, J.Y.; Young, T.H. Biomaterial mediated epithelial-mesenchymal interaction of salivary tissue under serum free condition. Biomaterials 2010, 31, 288-295.
122. Yang, T.L.; Young, T.H. The specificity of chitosan in promoting branching morphogenesis of progenitor salivary tissue. Biochem. Biophys. Res. Commun. 2009, 381, 466-470.
123. Umeda, Y.; Miyazaki, Y.; Shiinoki, H.; Higashiyama, S.; Nakanishi, Y.; Hieda, Y. Involvement of heparin-binding EGF-like growth factor and its processing by metalloproteinases in early epithelial morphogenesis of the submandibular gland. Dev. Biol. 2001, 237, 202-211.
124. Grobstein, C. Inductive epitheliomesenchymal interaction in cultured organ rudiments of the mouse. Science 1953, 118, 52-55.
125. Noguchi, Y.; Okamoto, A.; Kasama, T.; Imajoh-Ohmi, S.; Karatsu, T.; Nogawa, H. Lysophosphatidic acid cooperates with EGF in inducing branching morphogenesis of embryonic mouse salivary epithelium. Dev. Dyn. 2006, 235, 403-410.
126. Ignotz, R.A.; Massague, J. Transforming growth-factor-beta stimulates the expression of fibronectin and collagen and their incorporation into the extracellular-matrix. J. Biol. Chem. 1986, 261, 4337-4345.
127. Chalfoun, C.T.; Wirth, G.A.; Evans, G.R. Tissue engineered nerve constructs: Where do we stand? J. Cell. Mol. Med. 2006, 10, 309-317.
128. Freier, T.; Montenegro, R.; Shan Koh, H.; Shoichet, M.S. Chitin-based tubes for tissue engineering in the nervous system. Biomaterials 2005, 26, 4624-4632.
129. Haipeng, G.; Yinghui, Z.; Jianchun, L.; Yandao, G.; Nanming, Z.; Xiufang, Z. Studies on nerve cell affinity of chitosan-derived materials. J. Biomed. Mater. Res. 2000, 52, 285-295.
130. Matsuda, A.; Kobayashi, H.; Itoh, S.; Kataoka, K.; Tanaka, J. Immobilization of laminin peptide in molecularly aligned chitosan by covalent bonding. Biomaterials 2005, 26, 2273-2279.
131. Mingyu, C.; Kai, G.; Jiamou, L.; Yandao, G.; Nanming, Z.; Xiufang, Z. Surface modification and characterization of chitosan film blended with poly-L-lysine. J. Biomater. Appl. 2004, 19, 59-75.
132. Chavez-Delgado, M.E.; Mora-Galindo, J.; Gomez-Pinedo, U.; Feria-Velasco, A.; Castro-Castaneda, S.; Lopez-Dellamary Toral, F.A.; Luquin-De Anda, S.; Garcia-Segura, L.M.; Garcia-Estrada, J. Facial nerve regeneration through progesterone-loaded chitosan prosthesis. A preliminary report. J. Biomed. Mater. Res. B 2003, 67, 702-711.
133. Cheng, M.; Deng, J.; Yang, F.; Gong, Y.; Zhao, N.; Zhang, X. Study on physical properties and nerve cell affinity of composite films from chitosan and gelatin solutions. Biomaterials 2003, 24, 2871-2880.
134. Wang, X.; Hu, W.; Cao, Y.; Yao, J.; Wu, J.; Gu, X. Dog sciatic nerve regeneration across a 30-mm defect bridged by a chitosan/PGA artificial nerve graft. Brain 2005, 128, 1897-1910.
135. Fan, W.; Gu, J.; Hu, W.; Deng, A.; Ma, Y.; Liu, J.; Ding, F.; Gu, X. Repairing a 35-mm-long median nerve defect with a chitosan/PGA artificial nerve graft in the human: A case study. Microsurgery 2008, 28, 238-242.
136. Ding, F.; Wu, J.; Yang, Y.; Hu, W.; Zhu, Q.; Tang, X.; Liu, J.; Gu, X. Use of tissue engineered nerve grafts consisting of a chitosan/poly(lactic-co-glycolic acid)-based scaffold included with bridging 50-mm dog sciatic nerve gaps. Tissue Eng. Part A 2010 (in press).
137. Shim, S.; Ming, G.L. Roles of channels and receptors in the growth cone during PNS axonal regeneration. Exp. Neurol. 2010, 223, 38-44.
138. Yuan, Y.; Zhang, P.; Yang, Y.; Wang, X.; Gu, X. The interaction of Schwann cells with chitosan membranes and fibers in vitro. Biomaterials 2004, 25, 4273-4278.
139. Wang, W.; Itoh, S.; Konno, K.; Kikkawa, T.; Ichinose, S.; Sakai, K.; Ohkuma, T.; Watabe, K. Effects of Schwann cell alignment along the oriented electrospun chitosan nanofibers on nerve regeneration. J. Biomed. Mater. Res. A 2009, 91, 994-1005.
140. Katz, A.J.; Llull, R.; Hedrick, M.H.; Futrell, J.W. Emerging approaches to the tissue engineering of fat. Clin. Plast. Surg. 1999, 26, 587-603.
141. Beahm, E.K.; Walton, R.L.; Patrick, C.W., Jr. Progress in adipose tissue construct development. Clin. Plast. Surg. 2003, 30, 547-558.
142. Wu, X.; Black, L.; Santacana-Laffitte, G.; Patrick, C.W., Jr. Preparation and assessment of glutaraldehyde-crosslinked collagen-chitosan hydrogels for adipose tissue engineering. J. Biomed. Mater. Res. A 2007, 81, 59-65.
143. Laroche, G.; Marois, Y.; Guidoin, R.; King, M.W.; Martin, L.; How, T.; Douville, Y. Polyvinylidene fluoride (PVDF) as a biomaterial: From polymeric raw material to monofilament vascular suture. J. Biomed. Mater. Res. 1995, 29, 1523-1536.
144. Mori, T.; Irie, Y.; Nishimura, S.I.; Tokura, S.; Matsuura, M.; Okumura, M.; Kadosawa, T.; Fujinaga, T. Endothelial cell responses to chitin and its derivatives. J. Biomed. Mater. Res. 1998, 43, 469-472.
145. Malafaya, P.B.; Santos, T.C.; van Griensven, M.; Reis, R.L. Morphology, mechanical characterization and in vivo neo-vascularization of chitosan particle aggregated scaffolds architectures. Biomaterials 2008, 29, 3914-3926.
146. Chupa, J.M.; Foster, A.M.; Sumner, S.R.; Madihally, S.V.; Matthew, H.W. Vascular cell responses to polysaccharide materials: In vitro and in vivo evaluations. Biomaterials 2000, 21, 2315-2322.
147. Kratz, G.; Arnander, C.; Swedenborg, J.; Back, M.; Falk, C.; Gouda, I.; Larm, O. Heparin-chitosan complexes stimulate wound healing in human skin. Scand. J. Plast. Reconstr. Surg. Hand Surg. 1997, 31, 119-123.
148. Chandy, T.; Rao, G.H.; Wilson, R.F.; Das, G.S. Delivery of LMW heparin via surface coated chitosan/peg-alginate microspheres prevents thrombosis. Drug Deliv. 2002, 9, 87-96.
149. Fujita, M.; Ishihara, M.; Shimizu, M.; Obara, K.; Nakamura, S.; Kanatani, Y.; Morimoto, Y.; Takase, B.; Matsui, T.; Kikuchi, M.; Maehara, T. Therapeutic angiogenesis induced by controlled release of fibroblast growth factor-2 from injectable chitosan/non-anticoagulant heparin hydrogel in a rat hindlimb ischemia model. Wound Repair Regen. 2007, 15, 58-65.
150. Ishihara, M.; Obara, K.; Nakamura, S.; Fujita, M.; Masuoka, K.; Kanatani, Y.; Takase, B.; Hattori, H.; Morimoto, Y.; Ishihara, M.; Maehara, T.; Kikuchi, M. Chitosan hydrogel as a drug delivery carrier to control angiogenesis. J. Artif. Organs 2006, 9, 8-16.