Modulation of gene expression and collagen production of anterior cruciate ligament cells through cell shape changes on polycaprolactone/chitosan blends

Biomaterials

Volumn 31, Issue 17, 2010, Pages 4695-4705

  Shao, Hung Jen ^a   Lee, Yu Tsang ^a   Chen, Chiang Sang ^a,b   Wang, Jyh Horng ^c   Young, Tai Horng ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^b FAR EASTERN MEMORIAL HOSPITAL   (Taiwan)

^c NATIONAL TAIWAN UNIVERSITY HOSPITAL   (Taiwan)

Author keywords

Anterior cruciate ligament (ACL) cells; Blends; Cell shape; Chitosan; Gene expression; Polycaprolactone (PCL)

Indexed keywords

ANTERIOR CRUCIATE LIGAMENT CELLS; BLENDED MATERIALS; BLENDS; CELL SHAPE; CELL SHAPES; CELLULAR MORPHOLOGY; COLLAGEN SYNTHESIS; CYTOSKELETONS; LIGAMENT TISSUE ENGINEERING; MATERIAL SELECTION; MRNA EXPRESSION; PROTEIN PRODUCTION; PROTEIN SYNTHESIS; TRANSFORMING GROWTH FACTORS; WOUND HEALING;

BLENDING; CELL CULTURE; CELLS; CHITIN; CHITOSAN; COLLAGEN; LIGAMENTS; MORPHOLOGY; POLYCAPROLACTONE; TISSUE ENGINEERING;

GENE EXPRESSION;

CHITOSAN; COLLAGEN; COLLAGEN TYPE 1; COLLAGEN TYPE 3; CYTOCHALASIN C; MESSENGER RNA; POLYCAPROLACTONE; TRANSFORMING GROWTH FACTOR BETA1;

ANTERIOR CRUCIATE LIGAMENT; ARTICLE; CELL ADHESION; CELL PROLIFERATION; CELL SHAPE; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; CYTOSKELETON; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; INFRARED SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN SYNTHESIS; UPREGULATION;

ADULT; ANTERIOR CRUCIATE LIGAMENT; BIOCOMPATIBLE MATERIALS; CELL PROLIFERATION; CELL SHAPE; CELLS, CULTURED; CHITOSAN; COLLAGEN; GENE EXPRESSION; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, ATOMIC FORCE; POLYESTERS; POLYMERASE CHAIN REACTION; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; YOUNG ADULT;

EID: 77951092280     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biomaterials.2010.02.037     Document Type: Article

References (36)

1. Lo I.K.Y., Marchuk L.L., Hart D.A., Frank C.B. Comparison of mRNA levels for matrix molecules in normal and disrupted human anterior cruciate ligaments using reverse transcription polymerase chain reaction. J Orthop Res 1998, 16(4):421-428.
2. Riechert K., Labs K., Lindenhayn K., Sinha P. Semiquantitative analysis of types I and III collagen from tendons and ligaments in a rabbit model. J Orthop Sci 2001, 6(1):68-74.
3. Murray M.M., Martin S.D., Martin T.L., Spector M. Histological changes in the human anterior cruciate ligament after rupture. J Bone Joint Surg Am 2000, 82A(10):1387-1397.
4. Yoshida M., Fujii K. Differences in cellular properties and responses to growth factors between human ACL and MCL cells. J Orthop Sci 1999, 4(4):293-298.
5. Amiel D., Nagineni C.N., Choi S.H., Lee J. Interinsic-properties of ACL and MCL cells and their responses to growth-factors. Med Sci Sports Exerc 1995, 27(6):844-851.
6. Molloy T., Wang Y., Murrell G.A.C. The roles of growth factors in tendon and ligament healing. Sports Med 2003, 33(5):381-394.
7. Natsu-ume T., Nakamura N., Shino K., Toritsuka Y., Horibe S., Ochi T. Temporal and spatial expression of transforming growth factor-beta in the healing patellar ligament of the rat. J Orthop Res 1997, 837-843.
8. DesRosiers E.A., Yahia L., Rivard C.H. Proliferative and matrix synthesis response of canine anterior cruciate ligament fibroblasts submitted to combined growth factors. J Orthop Res 1996, 14(2):200-208.
9. Marui T., Niyibizi C., Georgescu H.I., Cao M., Kavalkovich K.W., Levine R.E., et al. Effect of growth factors on matrix synthesis by ligament fibroblasts. J Orthop Res 1997, 15(1):18-23.
10. Murray M.M., Rice K., Wright R.J., Spector M. The effect of selected growth factors on human anterior cruciate ligament cell interactions with a three-dimensional collagen-GAG scaffold. J Orthop Res 2003, 21(2):238-244.
11. Pascher A., Steinert A.F., Palmer G.D., Betz O., Gouze J.N., Gouze E., et al. Enhanced repair of the anterior cruciate ligament by in situ gene transfer: evaluation in an in vitro model. Mol Ther 2004, 10(2):327-336.
12. Zhang Y., Cheng X., Wang J., Wang Y., Shi B., Huang C., et al. Novel chitosan/collagen scaffold containing transforming growth factor-[beta]1 DNA for periodontal tissue engineering. Biochem Biophys Res Commun 2006, 344(1):362-369.
13. Ignotz R.A., Massague J. Transforming growth-factor-beta stimulates the expression of fibronectin and collagen and their incorporation into the extracellular-matrix. J Biol Chem 1986, 261(9):4337-4345.
14. Altman G.H., Horan R.L., Lu H.H., Moreau J., Martin I., Richmond J.C., et al. Silk matrix for tissue engineered anterior cruciate ligaments. Biomaterials 2002, 23(20):4131-4141.
15. Lin V.S., Lee M.C., O'Neal S., McKean J., Sung K.L.P. Ligament tissue engineering using synthetic biodegradable fiber scaffolds. Tissue Eng 1999, 5(5):443-451.
16. Lu H.H., Cooper J.A., Manuel S., Freeman J.W., Attawia M.A., Ko F.K., et al. Anterior cruciate ligament regeneration using braided biodegradable scaffolds: in vitro optimization studies. Biomaterials 2005, 26(23):4805-4816.
17. Okamoto Y., Shibazaki K., Minami S., Matsuhashi A., Tanioka S., Shigemasa Y. Evaluation of chitin and chitosan on open wound-healing in dogs. J Vet Med Sci 1995, 57(5):851-854.
18. Kojima K., Okamoto Y., Miyatake K., Fujise H., Shigemasa Y., Minami S. Effects of chitin and chitosan on collagen synthesis in wound healing. J Vet Med Sci 2004, 66(12):1595-1598.
19. Ueno H., Nakamura F., Murakami M., Okumura M., Kadosawa T., Fujinaga T. Evaluation effects of chitosan for the extracellular matrix production by fibroblasts and the growth factors production by macrophages. Biomaterials 2001, 22(15):2125-2130.
20. Fakhry A., Schneider G.B., Zaharias R., Senel S. Chitosan supports the initial attachment and spreading of osteoblasts preferentially over fibroblasts. Biomaterials 2004, 25(11):2075-2079.
21. Shao HJ, Chen CS, Lee YT, Wang JH, Young TH. The phenotypic responses of human anterior cruciate ligament cells culture on poly(e{open}-caprolactone) and chitosan. J Biomed Mater Res A, in press.
22. Chou L.S., Firth J.D., Uitto V.J., Brunette D.M. Substratum surface-topography altera cell-shape and regulates fibronectinmessenger-RNA level, messenger-RNA stability, secretion and assembly in human fibroblasts. J Cell Sci 1995, 108:1563-1573.
23. Thomas C.H., Collier J.H., Sfeir C.S., Healy K.E. Engineering gene expression and protein synthesis by modulation of nuclear shape. Proc Natl Acad Sci U S A 2002, 99(4):1972-1977.
24. Kidoaki S., Matsuda T. Shape-engineered fibroblasts: cell elasticity and actin cytoskeletal features characterized by fluorescence and atomic force microscopy. J Biomed Mater Res A 2007, 81A(4):803-810.
25. Li F., Li B., Wang Q.M., Wang J.H.C. Cell shape regulates collagen type I expression in human tendon fibroblasts. Cell Motil Cytoskeleton 2008, 65(4):332-341.
26. Wang J.H., Wei C.W., Liu H.C., Young T.H. Behavior of MG-63 cells on nylon/chitosan-blended membranes. J Biomed Mater Res A 2003, 64A(4):606-615.
27. Chuang W.Y., Young T.H., Yao C.H., Chiu W.Y. Properties of the poly(vinyl alcohol)/chitosan blend and its effect on the culture of fibroblast in vitro. Biomaterials 1999, 20(16):1479-1487.
28. Wang J.H., Hung C.H., Young T.H. Proliferation and differentiation of neural stem cells on lysine-alanine sequential polymer substrates. Biomaterials 2006, 27(18):3441-3450.
29. Folkman J., Moscona A. Role of cell shape in growth control. Nature 1978, 273(5661):345-349.
30. Varedi M., Tredget E.E., Scott P.G., Shen Y.J., Ghahary A. Alteration in cell morphology triggers transforming growth-factor-beta-1, collagenase, and tissue inhibitor of metalloproteinases-1 expression in nornal and hypertrophic scar fibroblasts. J Invest Dermatol 1995, 104(1):118-123.
31. Varedi M., Ghahary A., Scott P.G., Tredget E.E. Cytoskeleton regulates expression of genes for transforming growth factor-beta 1 and extracellular matrix proteins in dermal fibroblasts. J Cell Physiol 1997, 172(2):192-199.
32. Ouyang H.W., Goh J.C.H., Mo X.M., Teoh S.H., Lee E.H. Characterization of anterior cruciate ligament cells and bone marrow stromal cells on various biodegradable polymeric films. Mater Sci Eng C 2002, 63-69.
33. Verma I.M., Somia N. Gene therapy - promises, problems and prospects. Nature 1997, 389(6648):239-242.
34. Parkes R.J., Hart S.L. Adhesion molecules and gene transfer. Adv Drug Deliv Rev 2000, 44(2-3):135-152.
35. Cruz D.M.G., Ribelles J.L.G., Sanchez M.S. Blending polysaccharides with biodegradable polymers. I. Properties of chitosan/polycaprolactone blends. J Biomed Mater Res B Appl Biomater 2008, 85B(2):303-313.
36. Sarasam A., Madihally S.V. Characterization of chitosan-polycaprolactone blends for tissue engineering applications. Biomaterials 2005, 26(27):5500-5508.