Anti-diarrheal mechanism of the traditional remedy uzara via reduction of active chloride secretion

PLoS ONE

Volumn 6, Issue 3, 2011, Pages

  Schulzke, Jörg D ^a   Andres, Susanne ^a   Amasheh, Maren ^a   Fromm, Anja ^a   Günzel, Dorothee ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ANTIDIARRHEAL AGENT; CHLORIDE ION; CHOLERA TOXIN; CYCLIC AMP; FORSKOLIN; PLANT EXTRACT; UNCLASSIFIED DRUG; UZARA; UZARA EXTRACT; 4,4' DIISOTHIOCYANATOSTILBENE 2,2' DISULFONIC ACID; CARBACHOL; CHLORIDE; NYSTATIN; OUABAIN; SODIUM;

ADULT; AGED; ARTICLE; CELL LEVEL; CELL MEMBRANE RESISTANCE; CELL STRAIN HT29; CHLORIDE CURRENT; CHLORIDE TRANSPORT; COLON MUCOSA; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG MECHANISM; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; INHIBITION KINETICS; MEDICINAL PLANT; SHORT CIRCUIT CURRENT; SODIUM TRANSPORT; SPECTROSCOPY; TWO PATH IMPEDANCE SPECTROSCOPY; UZARA; ASCLEPIADACEAE; CHANNEL GATING; CHEMISTRY; COLON; DRUG EFFECT; FEMALE; IMPEDANCE; MALE; METABOLISM; MIDDLE AGED; PATHOLOGY; TRADITIONAL MEDICINE; TRANSPORT AT THE CELLULAR LEVEL;

4,4'-DIISOTHIOCYANOSTILBENE-2,2'-DISULFONIC ACID; ADULT; AGED; ANTIDIARRHEALS; ASCLEPIADACEAE; BIOLOGICAL TRANSPORT; CARBACHOL; CHLORIDES; CHOLERA TOXIN; COLON; CYCLIC AMP; ELECTRIC IMPEDANCE; FEMALE; FORSKOLIN; HT29 CELLS; HUMANS; ION CHANNEL GATING; MALE; MEDICINE, TRADITIONAL; MIDDLE AGED; NYSTATIN; OUABAIN; PLANT EXTRACTS; SODIUM; SODIUM-POTASSIUM-EXCHANGING ATPASE;

EID: 79953297105     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0018107     Document Type: Article

References (33)

1. Kunzelmann K, Mall M, (2002) Electrolyte transport in the mammalian colon: mechanisms and implications for disease. Physiol Rev 82: 245-289.
2. Desjeux JF, Briend A, Butzner JD, (1997) Oral rehydration solution in the year 2000: pathophysiology, efficacy and effectiveness. Baillieres Clin Gastroenterol 11: 509-527.
3. Mall M, Bleich M, Schurlein M, Kuhr J, Seydewitz HH, et al. (1998) Cholinergic ion secretion in human colon requires coactivation by cAMP. Am J Physiol- Gastrointest Liver Physiol 275: G1274-G1281.
4. Flores CA, Melvin JE, Figueroa CD, Sepúlveda FV, (2007) Abolition of Ca2+-mediated intestinal anion secretion and increased stool dehydration in mice lacking the intermediate conductance Ca2+-dependent K+ channel Kcnn4. J Physiol 583: 705-717.
5. Rosenthal R, Milatz S, Krug SM, Oelrich B, Schulzke JD, et al. (2010) Claudin-2, a component of the tight junction, forms a paracellular water channel. J Cell Sci 123: 1913-1921.
6. Epple HJ, Kreusel KM, Hanski C, Schulzke JD, Riecken EO, et al. (1997) Differential stimulation of intestinal mucin secretion by cholera toxin and carbachol. Pflügers Arch 433: 638-647.
7. Kreusel KM, Fromm M, Schulzke JD, Hegel U, (1991) Cl- secretion in epithelial monolayers of mucus-forming human colon cells (HT-29/B6). Am J Physiol 261: C574-C582.
8. van Wyk BE, (2008) A broad review of commercially important southern African medicinal plants. J Ethnopharmacol 119: 342-355.
9. Ghorbani M, Kaloga M, Frey HH, Mayer G, Eich E, (1997) Phytochemical reinvestigation of Xysmalobium undulatum roots (Uzara). Planta Med 63: 343-346.
10. Brown L, Erdmann E, Thomas R, (1983) Digitalis structure-activity relationship analyses. Conclusions from indirect binding studies with cardiac (Na+ + K+)-ATPase. Biochem Pharmacol 32: 2767-2774.
11. Raymond-Hamet, (1930) Analyse physiologique de L'action intestinale de l'Uzara. C R Hebd Seances Acad Sci 190: 821-823.
12. Geβner O, (1930) Über Uzara. II Mitteilung: Über den Wirkungsmechanismus von Uzara bei glattmuskeligen Organen, insbesondere am Darm. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol 148: 353-368.
13. Schulz V, Hänsel R, Tyler VE, (2001) Rational phytotherapy: a physician's guide to herbal medicine. Springer-Verlag.
14. Schmitz H, Rokos K, Florian P, Gitter AH, Fromm M, Scholz P, Ullrich R, Zeitz M, Pauli G, Schulzke JD, (2002) Supernatants of HIV-infected immune cells affect the barrier function of human HT-29/B6 intestinal epithelial cells. Aids 16: 983-991.
15. Zeissig S, Bojarski C, Bürgel N, Mankertz J, Zeitz M, et al. (2004) Downregulation of epithelial apoptosis and barrier repair in active Crohn's disease by TNFalpha antibody treatment. Gut 53: 1295-1302.
16. Stockmann M, Fromm M, Schmitz H, Schmidt W, Riecken EO, et al. (1998) Duodenal biopsies of HIV-infected patients with diarrhoea exhibit epithelial barrier defects but no active secretion. Aids 12: 43-51.
17. Sadrzadeh SM, Vincenzi FF, Hinds TR, (1993) Simultaneous measurement of multiple membrane ATPases in microtiter plates. J Pharmacol Toxicol Methods 30: 103-110.
18. Krug SM, Fromm M, Günzel D, (2009) Two-path impedance spectroscopy for measuring paracellular and transcellular epithelial resistance. Biophys J 97: 2202-2211.
19. Dharmsathaphorn K, Pandol SJ, (1986) Mechanism of chloride secretion induced by carbachol in a colonic epithelial cell line. J Clin Invest 77: 348-354.
20. Weymer A, Huott P, Liu W, McRoberts JA, Dharmsathaphorn K, (1985) Chloride secretory mechanism induced by prostaglandin E1 in a colonic epithelial cell line. J Clin Invest 76: 1828-1836.
21. Bajnath RB, Augeron C, Laboisse CL, Bijman J, de Jonge HR, et al. (1991) Electrophysiological studies of forskolin-induced changes in ion transport in the human colon carcinoma cell line HT-29 cl.19A: lack of evidence for a cAMP-activated basolateral K+ conductance. J Membr Biol 122: 239-250.
22. Bridges RJ, Rummel W, Simon B, (1983) Forskolin induced chloride secretion across the isolated mucosa of rat colon descendens. Naunyn Schmiedebergs Arch Pharmacol 323: 355-360.
23. Kimberg DV, Field M, Johnson J, Henderson A, Gershon E, (1971) Stimulation of intestinal mucosal adenyl cyclase by cholera enterotoxin and prostaglandins. J Clin Invest 50: 1218-1230.
24. Preston P, Wartosh L, Günzel D, Fromm M, Kongsuphol P, et al. (2010) Disruption of the K+-channel β-subunit KCNE3 reveals an important role in colonic and tracheal Cl- transport. J Biol Chem 285: 7165-7175.
25. Strabel D, Diener M, (1995) Evidence against direct activation of chloride secretion by carbachol in the rat distal colon. Eur J Pharmacol 274: 181-191.
26. Smith PL, Sullivan SK, McCabe RD, (1986) Concentration-dependent effects of disulfunic stilbenes on colonic chloride transport. Am J Physiol- Gastrointest Liver Physiol 250: G44-G49.
27. Brayden DJ, Krouse ME, Law T, Wine JJ, (1993) Stilbenes stimulate T84 Cl- secretion by elevating Ca2+. Am J Physiol- Gastrointest Liver Physiol 264: G325-G333.
28. Lencer WI, (2001) Microbes and microbial toxins: paradigms for microbial-mucosal toxins. V. Cholera: invasion of the intestinal epithelial barrier by a stably folded protein toxin. Am J Physiol- Gastrointest Liver Physiol 280: G781-G786.
29. Mandal A, Delamere NA, Shahidullah M, (2008) Ouabain-induced stimulation of sodium-hydrogen exchange in rat optic nerve astrocytes. Am J Physiol- Cell Physiol 295: C100-C110.
30. Abbott AJ, Holoubek CG, Martin RA, (1998) Inhibition of Na+,K+-ATPase by the cardenolide 6′-O-(E-4-hydroxycinnamoyl) desglucouzarin. Biochem Biophys Res Commun 251: 256-259.
31. Thürmann PA, Neff A, Fleisch J, (2004) Interference of Uzara glycosides in assays of digitalis glycosides. Int J Clin Pharmacol Ther 42: 281-284.
32. Schmiedl S, Szymanski J, Ritter A, Tenter U, Alken RG, et al. (2006) Pharmakokinetics of uzara glycosides and cross-reactivity in digitalis immunoassays. Int J Clin Pharmacol Ther 44: 526.
33. Ritter A, Schmiedl S, Szymanski J, Tenter U, Alken RG, et al. (2006) Cardiovascular effects of Uzara Lösung N in healthy volunteers in comparison to placebo and digoxin. Int J Clin Pharmacol Ther 44: 529.